CatLogenzyme kinetics catalog

Values CatLog read from this paper

Probing the interactions between the folding elements early in the folding of Escherichia coli dihydrofolate reductase by systematic sequence perturbation analysis

PMID 15740745doi:10.1016/j.jmb.2005.01.033Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 52 records (156 values) from this paper: dihydrofolate reductase (DHFR) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dihydrofolate reductase (DHFR)Escherichia colidihydrofolate6.188.9 × 10⁻⁴6.92 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155AEscherichia colidihydrofolate5.20.001443.62 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155DEscherichia colidihydrofolate7.550.00223.43 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155EEscherichia colidihydrofolate5.80.001763.29 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155KEscherichia colidihydrofolate4.677.5 × 10⁻⁴6.22 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155LEscherichia colidihydrofolate4.980.001692.95 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155QEscherichia colidihydrofolate7.380.001564.73 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155REscherichia colidihydrofolate8.070.002393.37 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155SEscherichia colidihydrofolate5.979.6 × 10⁻⁴6.24 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155TEscherichia colidihydrofolate4.920.001273.86 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155VEscherichia colidihydrofolate4.80.00153.19 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155WEscherichia colidihydrofolate6.750.002642.55 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant I155YEscherichia colidihydrofolate6.030.001514 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59CEscherichia colidihydrofolate4.970.001084.59 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59EEscherichia colidihydrofolate5.570.003451.62 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59GEscherichia colidihydrofolate3.920.001852.12 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59HEscherichia colidihydrofolate1.450.00111.31 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59IEscherichia colidihydrofolate4.180.003091.36 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59KEscherichia colidihydrofolate4.30.0136320pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59LEscherichia colidihydrofolate3.450.001821.9 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59MEscherichia colidihydrofolate3.650.002241.63 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59PEscherichia colidihydrofolate5.680.002432.34 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59QEscherichia colidihydrofolate4.930.002022.45 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59SEscherichia colidihydrofolate4.770.002621.82 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59TEscherichia colidihydrofolate10.30.004912.1 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59VEscherichia colidihydrofolate6.730.001823.69 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant N59WEscherichia colidihydrofolate5.020.00942530pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148AEscherichia colidihydrofolate4.85 × 10⁻⁴9.61 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148EEscherichia colidihydrofolate6.670.001096.12 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148KEscherichia colidihydrofolate6.884.1 × 10⁻⁴1.669 × 10⁴pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148NEscherichia colidihydrofolate7.828.3 × 10⁻⁴9.46 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148PEscherichia colidihydrofolate5.727.1 × 10⁻⁴8.04 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148REscherichia colidihydrofolate4.550.00162.84 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148TEscherichia colidihydrofolate8.220.003232.54 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant S148VEscherichia colidihydrofolate5.856.6 × 10⁻⁴8.92 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40AEscherichia colidihydrofolate9.570.001775.42 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40FEscherichia colidihydrofolate13.530.004463.04 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40HEscherichia colidihydrofolate9.530.007551.26 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40IEscherichia colidihydrofolate3.80.00182.11 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40LEscherichia colidihydrofolate4.830.001752.77 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40MEscherichia colidihydrofolate3.70.002231.66 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40NEscherichia colidihydrofolate3.420.002081.64 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40REscherichia colidihydrofolate7.820.001634.78 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant V40SEscherichia colidihydrofolate5.50.002212.49 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30AEscherichia colidihydrofolate14.50.002146.79 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30EEscherichia colidihydrofolate11.580.001358.59 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30HEscherichia colidihydrofolate13.120.001946.77 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30MEscherichia colidihydrofolate11.90.002115.65 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30NEscherichia colidihydrofolate13.980.002485.63 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30REscherichia colidihydrofolate14.080.00275.21 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30SEscherichia colidihydrofolate150.002097.17 × 10³pH 7, 15 °COpen
dihydrofolate reductase (DHFR)variant W30YEscherichia colidihydrofolate13.470.002555.28 × 10³pH 7, 15 °COpen