Values CatLog read from this paper
Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants
PMID 20045086doi:10.1016/j.bbapap.2009.12.011Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (33 values) from this paper: dihydrofolate reductase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrofolate reductase | Escherichia coli | 7,8-dihydrofolate | 24.8 | 0.0013 | 1.92 × 10⁴ | pH 7, 25 °C | Open record: dihydrofolate reductase, 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔA145 (Δ145) | Escherichia coli | 7,8-dihydrofolate | 24 | 8 × 10⁻⁴ | 2.94 × 10⁴ | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔA145 (Δ145), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔG121 (Δ121) | Escherichia coli | 7,8-dihydrofolate | 1.7 | 0.0108 | 160 | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔG121 (Δ121), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔG121/ΔA145 (Δ121/Δ145) | Escherichia coli | 7,8-dihydrofolate | 0.22 | 0.0073 | 30 | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔG121/ΔA145 (Δ121/Δ145), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔG67 (Δ67) | Escherichia coli | 7,8-dihydrofolate | 16.5 | 0.0051 | 3.3 × 10³ | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔG67 (Δ67), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔG67/ΔA145 (Δ67/Δ145) | Escherichia coli | 7,8-dihydrofolate | 41.1 | 0.0048 | 8.6 × 10³ | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔG67/ΔA145 (Δ67/Δ145), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔG67/ΔG121 (Δ67/Δ121) | Escherichia coli | 7,8-dihydrofolate | 0.38 | 0.0056 | 70 | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔG67/ΔG121 (Δ67/Δ121), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔR52 (Δ52) | Escherichia coli | 7,8-dihydrofolate | 96.3 | 0.1161 | 830 | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔR52 (Δ52), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔR52/ΔA145 (Δ52/Δ145) | Escherichia coli | 7,8-dihydrofolate | 31.9 | 0.0777 | 410 | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔR52/ΔA145 (Δ52/Δ145), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔR52/ΔG121 (Δ52/Δ121) | Escherichia coli | 7,8-dihydrofolate | 0.37 | 0.0512 | 10 | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔR52/ΔG121 (Δ52/Δ121), 7,8-dihydrofolate |
| dihydrofolate reductasevariant ΔR52/ΔG67 (Δ52/Δ67) | Escherichia coli | 7,8-dihydrofolate | 23.1 | 0.0502 | 460 | pH 7, 25 °C | Open record: dihydrofolate reductase variant ΔR52/ΔG67 (Δ52/Δ67), 7,8-dihydrofolate |