Values CatLog read from this paper
Role of the active-site carboxylate in dihydrofolate reductase: kinetic and spectroscopic studies of the aspartate 26-->asparagine mutant of the Lactobacillus casei enzyme
PMID 7893701doi:10.1021/bi00009a018Open in Browse
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CatLog read 8 records (20 values) from this paper: dihydrofolate reductase from Lactobacillus casei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrofolate reductase | Lactobacillus casei | 7,8-dihydrofolate | 25 | 0.001 | 2.5 × 10⁴ | pH 5.5, 25 °C | Open record: dihydrofolate reductase, 7,8-dihydrofolate |
| dihydrofolate reductase | Lactobacillus casei | 7,8-dihydrofolate | 10 | 0.001 | 1 × 10⁴ | pH 7.5, 25 °C | Open record: dihydrofolate reductase, 7,8-dihydrofolate |
| dihydrofolate reductase | Lactobacillus casei | folic acid | — | 6 × 10⁻⁴ | — | pH 5.5, 25 °C | Open record: dihydrofolate reductase, folic acid |
| dihydrofolate reductase | Lactobacillus casei | folic acid | 0.06 | 0.029 | 2 | pH 7.5, 25 °C | Open record: dihydrofolate reductase, folic acid |
| dihydrofolate reductasevariant D26N | Lactobacillus casei | 7,8-dihydrofolate | 5.5 | 0.0016 | 3.4 × 10³ | pH 5.5, 25 °C | Open record: dihydrofolate reductase variant D26N, 7,8-dihydrofolate |
| dihydrofolate reductasevariant D26N | Lactobacillus casei | 7,8-dihydrofolate | 1.1 | 0.0014 | 790 | pH 7.5, 25 °C | Open record: dihydrofolate reductase variant D26N, 7,8-dihydrofolate |
| dihydrofolate reductasevariant D26N | Lactobacillus casei | folic acid | — | 6 × 10⁻⁴ | — | pH 5.5, 25 °C | Open record: dihydrofolate reductase variant D26N, folic acid |
| dihydrofolate reductasevariant D26N | Lactobacillus casei | folic acid | 0.0024 | 0.016 | 0.15 | pH 7.5, 25 °C | Open record: dihydrofolate reductase variant D26N, folic acid |