CatLogenzyme kinetics catalog

Values CatLog read from this paper

Increasing methotrexate resistance by combination of active-site mutations in human dihydrofolate reductase

PMID 17868689doi:10.1016/j.jmb.2007.07.076Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 11 records (31 values) from this paper: dihydrofolate reductase (hDHFR) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dihydrofolate reductase (hDHFR)Homo sapiens5,6-dihydrofolate10——pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31A/F34V/Q35HHomo sapiens5,6-dihydrofolate1.10.0043300pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31G/Q35NHomo sapiens5,6-dihydrofolate4.30.00172.6 × 10³pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31PHomo sapiens5,6-dihydrofolate6.40.00154.3 × 10³pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31P/Q35EHomo sapiens5,6-dihydrofolate1.70.0027600pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31P/Q35HHomo sapiens5,6-dihydrofolate1.70.0021800pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31R/F34A/Q35NHomo sapiens5,6-dihydrofolate0.50.0022200pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31R/F34T/Q35RHomo sapiens5,6-dihydrofolate10.002500pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31R/F34T/Q35SHomo sapiens5,6-dihydrofolate10.0017600pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31R/Q35EHomo sapiens5,6-dihydrofolate1.36.9 × 10⁻⁴1.9 × 10³pH 7.6, 23 °COpen
dihydrofolate reductase (hDHFR)variant F31S/Q35EHomo sapiens5,6-dihydrofolate9.40.00332.8 × 10³pH 7.6, 23 °COpen