Values CatLog read from this paper

Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions

PMID 15944418doi:10.1093/jb/mvi079Open in Browse

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CatLog read 14 records (42 values) from this paper: dihydrofolate reductase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
dihydrofolate reductasevariant M42A	Escherichia coli	dihydrofolate	14	0.0013	1.08 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42A, dihydrofolate
dihydrofolate reductasevariant M42C	Escherichia coli	dihydrofolate	30.4	0.00194	1.57 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42C, dihydrofolate
dihydrofolate reductasevariant M42E	Escherichia coli	dihydrofolate	15.9	0.00597	2.7 × 10³	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42E, dihydrofolate
dihydrofolate reductasevariant M42G	Escherichia coli	dihydrofolate	13	0.00118	1.1 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42G, dihydrofolate
dihydrofolate reductasevariant M42H	Escherichia coli	dihydrofolate	26.6	0.00193	1.38 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42H, dihydrofolate
dihydrofolate reductasevariant M42I	Escherichia coli	dihydrofolate	12.4	6.1 × 10⁻⁴	2.03 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42I, dihydrofolate
dihydrofolate reductasevariant M42L	Escherichia coli	dihydrofolate	18.9	0.00112	1.69 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42L, dihydrofolate
dihydrofolate reductasevariant M42P	Escherichia coli	dihydrofolate	24	8.3 × 10⁻⁴	2.89 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42P, dihydrofolate
dihydrofolate reductasevariant M42Q	Escherichia coli	dihydrofolate	24.3	0.00227	1.07 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42Q, dihydrofolate
dihydrofolate reductasevariant M42S	Escherichia coli	dihydrofolate	22.6	0.00227	1 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42S, dihydrofolate
dihydrofolate reductasevariant M42T	Escherichia coli	dihydrofolate	18.7	8.2 × 10⁻⁴	2.28 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42T, dihydrofolate
dihydrofolate reductasevariant M42V	Escherichia coli	dihydrofolate	15.7	9.1 × 10⁻⁴	1.73 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42V, dihydrofolate
dihydrofolate reductasevariant M42W	Escherichia coli	dihydrofolate	104.6	0.045	2.3 × 10³	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42W, dihydrofolate
dihydrofolate reductasevariant M42Y	Escherichia coli	dihydrofolate	45.5	0.00178	2.56 × 10⁴	pH 7, 25 °C	Open record: dihydrofolate reductase variant M42Y, dihydrofolate