Values CatLog read from this paper
Breaking symmetry: mutations engineered into R67 dihydrofolate reductase, a D2 symmetric homotetramer possessing a single active site pore
PMID 12501195doi:10.1021/bi026676jOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: R67 dihydrofolate reductase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| R67 dihydrofolate reductasevariant S59A/H362L (Quad3) | — | dihydrofolate | 0.81 | 0.0067 | 120 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/H362L (Quad3), dihydrofolate |
| R67 dihydrofolate reductasevariant S59A/H362L (Quad3) | — | NADPH | — | 0.0044 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/H362L (Quad3), NADPH |
| R67 dihydrofolate reductasevariant S59A/Q67H/H362L (Q67H:1) | — | dihydrofolate | 0.23 | 0.0036 | 64 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/H362L (Q67H:1), dihydrofolate |
| R67 dihydrofolate reductasevariant S59A/Q67H/H362L (Q67H:1) | — | NADPH | — | 0.0013 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/H362L (Q67H:1), NADPH |
| R67 dihydrofolate reductasevariant S59A/Q67H/H362L/Q367H (Q67H:1+4) | — | dihydrofolate | 0.15 | 0.0026 | 58 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/H362L/Q367H (Q67H:1+4), dihydrofolate |
| R67 dihydrofolate reductasevariant S59A/Q67H/H362L/Q367H (Q67H:1+4) | — | NADPH | — | 9.5 × 10⁻⁴ | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/H362L/Q367H (Q67H:1+4), NADPH |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/H362L (Q67H:1+2) | — | dihydrofolate | 0.21 | 0.0036 | 58 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q167H/H362L (Q67H:1+2), dihydrofolate |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/H362L (Q67H:1+2) | — | NADPH | — | 2.8 × 10⁻⁴ | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q167H/H362L (Q67H:1+2), NADPH |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L (Q67H:1+2+3) | — | dihydrofolate | 0.09 | 0.0075 | 12 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q167H/Q267H/H362L (Q67H:1+2+3), dihydrofolate |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L (Q67H:1+2+3) | — | NADPH | — | 4.5 × 10⁻⁴ | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q167H/Q267H/H362L (Q67H:1+2+3), NADPH |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L/Q367H (Q67H:1+2+3+4) | — | dihydrofolate | 0.1 | 1.3 × 10⁻⁴ | 770 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q167H/Q267H/H362L/Q367H (Q67H:1+2+3+4), dihydrofolate |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q167H/Q267H/H362L/Q367H (Q67H:1+2+3+4) | — | NADPH | — | 2.6 × 10⁻⁵ | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q167H/Q267H/H362L/Q367H (Q67H:1+2+3+4), NADPH |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q267H/H362L (Q67H:1+3) | — | dihydrofolate | 0.23 | 0.011 | 21 calc. | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q267H/H362L (Q67H:1+3), dihydrofolate |
| R67 dihydrofolate reductasevariant S59A/Q67H/Q267H/H362L (Q67H:1+3) | — | NADPH | — | 0.0018 | — | pH 7, 30 °C | Open record: R67 dihydrofolate reductase variant S59A/Q67H/Q267H/H362L (Q67H:1+3), NADPH |