Values CatLog read from this paper

Role of lysine-54 in determining cofactor specificity and binding in human dihydrofolate reductase

PMID 2124504doi:10.1021/bi00487a011Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 6 records (14 values) from this paper: dihydrofolate reductase (hDHFR) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
dihydrofolate reductase (hDHFR)	Homo sapiens	7,8-dihydrofolic acid	—	3.2 × 10⁻⁵	—	pH 7.5	Open record: dihydrofolate reductase (hDHFR), 7,8-dihydrofolic acid
dihydrofolate reductase (hDHFR)	Homo sapiens	nicotinamide adenine dinucleotide (reduced)	1.2	0.018	67	pH 7.5	Open record: dihydrofolate reductase (hDHFR), nicotinamide adenine dinucleotide (reduced)
dihydrofolate reductase (hDHFR)	Homo sapiens	nicotinamide adenine dinucleotide phosphate (reduced)	7.4	2.6 × 10⁻⁴	2.85 × 10⁴	pH 7.5	Open record: dihydrofolate reductase (hDHFR), nicotinamide adenine dinucleotide phosphate (reduced)
dihydrofolate reductase (hDHFR)variant K54Q	Homo sapiens	7,8-dihydrofolic acid	—	1.8 × 10⁻⁴	—	pH 7.5	Open record: dihydrofolate reductase (hDHFR) variant K54Q, 7,8-dihydrofolic acid
dihydrofolate reductase (hDHFR)variant K54Q	Homo sapiens	nicotinamide adenine dinucleotide (reduced)	2	0.071	28	pH 7.5	Open record: dihydrofolate reductase (hDHFR) variant K54Q, nicotinamide adenine dinucleotide (reduced)
dihydrofolate reductase (hDHFR)variant K54Q	Homo sapiens	nicotinamide adenine dinucleotide phosphate (reduced)	18.5	0.015	1.2 × 10³	pH 7.5	Open record: dihydrofolate reductase (hDHFR) variant K54Q, nicotinamide adenine dinucleotide phosphate (reduced)