Values CatLog read from this paper

Role of Lys-32 residues in R67 dihydrofolate reductase probed by asymmetric mutations

PMID 15333636doi:10.1074/jbc.m404484200Open in Browse

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CatLog read 10 records (22 values) from this paper: R67 dihydrofolate reductase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
R67 dihydrofolate reductase	—	7,8-dihydrofolate	0.85	0.0076	110	pH 7, 30 °C	Open record: R67 dihydrofolate reductase, 7,8-dihydrofolate
R67 dihydrofolate reductase	—	nicotinamide adenine dinucleotide phosphate (reduced)	—	0.0039	220	pH 7, 30 °C	Open record: R67 dihydrofolate reductase, nicotinamide adenine dinucleotide phosphate (reduced)
R67 dihydrofolate reductasevariant K32M:1	—	7,8-dihydrofolate	0.56	0.006	93	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1, 7,8-dihydrofolate
R67 dihydrofolate reductasevariant K32M:1	—	nicotinamide adenine dinucleotide phosphate (reduced)	—	0.0036	160	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1, nicotinamide adenine dinucleotide phosphate (reduced)
R67 dihydrofolate reductasevariant K32M:1+2	—	7,8-dihydrofolate	0.2	0.0144	14	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1+2, 7,8-dihydrofolate
R67 dihydrofolate reductasevariant K32M:1+2	—	nicotinamide adenine dinucleotide phosphate (reduced)	—	0.0171	12	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1+2, nicotinamide adenine dinucleotide phosphate (reduced)
R67 dihydrofolate reductasevariant K32M:1+3	—	7,8-dihydrofolate	—	—	9	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1+3, 7,8-dihydrofolate
R67 dihydrofolate reductasevariant K32M:1+3	—	nicotinamide adenine dinucleotide phosphate (reduced)	—	—	26	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1+3, nicotinamide adenine dinucleotide phosphate (reduced)
R67 dihydrofolate reductasevariant K32M:1+4	—	7,8-dihydrofolate	0.1	0.0105	10	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1+4, 7,8-dihydrofolate
R67 dihydrofolate reductasevariant K32M:1+4	—	nicotinamide adenine dinucleotide phosphate (reduced)	—	0.0054	19	pH 7, 30 °C	Open record: R67 dihydrofolate reductase variant K32M:1+4, nicotinamide adenine dinucleotide phosphate (reduced)