CatLogenzyme kinetics catalog

Values CatLog read from this paper

Substrate and inhibitor specificity of Mycobacterium avium dihydrofolate reductase

PMID 17542991doi:10.1111/j.1742-4658.2007.05855.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (60 values) from this paper: dihydrofolate reductase (DHFR) from Mycobacterium avium.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dihydrofolate reductase (DHFR)Mycobacterium aviumdihydrofolate20.57 × 10⁻⁴2.9 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)Mycobacterium aviumNADPH26.80.001551.73 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31AMycobacterium aviumdihydrofolate0.860.03723pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31AMycobacterium aviumNADPH0.596.8 × 10⁻⁴380pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31EMycobacterium aviumdihydrofolate3.580.001921.87 × 10³pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31EMycobacterium aviumNADPH2.050.002011.02 × 10³pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31LMycobacterium aviumdihydrofolate0.0250.1980.13pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31LMycobacterium aviumNADPH0.020.0014413.9pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31NMycobacterium aviumdihydrofolate5.030.002082.4 × 10³pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31NMycobacterium aviumNADPH8.57.7 × 10⁻⁴1.1 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31QMycobacterium aviumdihydrofolate1.50.00232650pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant D31QMycobacterium aviumNADPH2.186.5 × 10⁻⁴3.35 × 10³pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant L32AMycobacterium aviumdihydrofolate16.29.6 × 10⁻⁴1.69 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant L32AMycobacterium aviumNADPH17.80.001391.28 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant L32DMycobacterium aviumdihydrofolate7.20.005121.41 × 10³pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant L32DMycobacterium aviumNADPH108.4 × 10⁻⁴1.19 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant L32FMycobacterium aviumdihydrofolate216.8 × 10⁻⁴3.09 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant L32FMycobacterium aviumNADPH23.20.001321.76 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant V76AMycobacterium aviumdihydrofolate18.77.8 × 10⁻⁴2.4 × 10⁴pH 7, 30 °COpen
dihydrofolate reductase (DHFR)variant V76AMycobacterium aviumNADPH23.70.001561.52 × 10⁴pH 7, 30 °COpen