Values CatLog read from this paper
Evolutional design of a hyperactive cysteine- and methionine-free mutant of Escherichia coli dihydrofolate reductase
PMID 16510443doi:10.1074/jbc.m508823200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (32 values) from this paper: dihydrofolate reductase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrofolate reductase | Escherichia coli | dihydrofolateTable 1 | 5.3 | 0.0012 | 4.4 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Escherichia coli | dihydrofolateenzyme incubated 24 h at 4 °C in 0.1 M H2O2 before the assay | 3.1 | 0.0014 | 2.2 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Escherichia coli | dihydrofolatein the text | — | — | 4.4 × 10³ | pH 7, 15 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductasevariant C85A/C152S (AS-DHFR) | Escherichia coli | dihydrofolate | 4.9 | 0.0012 | 4.1 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant C85A/C152S (AS-DHFR), dihydrofolate |
| dihydrofolate reductasevariant M16I | Escherichia coli | dihydrofolate | 17 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M16I, dihydrofolate |
| dihydrofolate reductasevariant M16K | Escherichia coli | dihydrofolate | 18 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M16K, dihydrofolate |
| dihydrofolate reductasevariant M16L | Escherichia coli | dihydrofolate | 7 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M16L, dihydrofolate |
| dihydrofolate reductasevariant M16N | Escherichia coli | dihydrofolate | 16.1 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M16N, dihydrofolate |
| dihydrofolate reductasevariant M1A/C85A/C152S (M1MA) | Escherichia coli | dihydrofolate | 4.6 | 0.0013 | 3.5 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1A/C85A/C152S (M1MA), dihydrofolate |
| dihydrofolate reductasevariant M1A/M16F/M20L/M42Y/M92F/C85A/C152S (AFLYF) | Escherichia coli | dihydrofolateTable 1 | 25.8 | 0.003 | 8.6 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1A/M16F/M20L/M42Y/M92F/C85A/C152S (AFLYF), dihydrofolate |
| dihydrofolate reductasevariant M1A/M16F/M20L/M42Y/M92F/C85A/C152S (AFLYF) | Escherichia coli | dihydrofolatein the text | — | — | 8.6 × 10³ | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1A/M16F/M20L/M42Y/M92F/C85A/C152S (AFLYF), dihydrofolate |
| dihydrofolate reductasevariant M1A/M16N/M20L/M42Y/M92F/C85A/C152S (ANLYF) | Escherichia coli | dihydrofolate | 40.5 | 0.0083 | 4.9 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1A/M16N/M20L/M42Y/M92F/C85A/C152S (ANLYF), dihydrofolate |
| dihydrofolate reductasevariant M1A/M16N/M20L/M42Y/M92F/C85A/C152S (ANLYF) | Escherichia coli | dihydrofolateenzyme incubated 24 h at 4 °C in 0.1 M H2O2 before the assay | 37.9 | 0.0089 | 4.3 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1A/M16N/M20L/M42Y/M92F/C85A/C152S (ANLYF), dihydrofolate |
| dihydrofolate reductasevariant M1A/M16S/M20L/M42Y/M92F/C85A/C152S (ASLYF) | Escherichia coli | dihydrofolate | 16.2 | 0.0038 | 4.3 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1A/M16S/M20L/M42Y/M92F/C85A/C152S (ASLYF), dihydrofolate |
| dihydrofolate reductasevariant M1P/C85A/C152S (M1MP) | Escherichia coli | dihydrofolate | 4.8 | 0.0012 | 4.0 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1P/C85A/C152S (M1MP), dihydrofolate |
| dihydrofolate reductasevariant M1S/C85A/C152S (M1MS) | Escherichia coli | dihydrofolate | 5.3 | 0.0025 | 2.1 × 10³ calc. | pH 7, 15 °C | Open record: dihydrofolate reductase variant M1S/C85A/C152S (M1MS), dihydrofolate |
| dihydrofolate reductasevariant M42I | Escherichia coli | dihydrofolate | 6.2 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M42I, dihydrofolate |
| dihydrofolate reductasevariant M42L | Escherichia coli | dihydrofolate | 11.8 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M42L, dihydrofolate |
| dihydrofolate reductasevariant M42Y | Escherichia coli | dihydrofolate | 20.2 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M42Y, dihydrofolate |
| dihydrofolate reductasevariant M92F | Escherichia coli | dihydrofolate | 6.8 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M92F, dihydrofolate |
| dihydrofolate reductasevariant M92I | Escherichia coli | dihydrofolate | 7.9 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M92I, dihydrofolate |
| dihydrofolate reductasevariant M92L | Escherichia coli | dihydrofolate | 6.3 | — | — | pH 7, 15 °C | Open record: dihydrofolate reductase variant M92L, dihydrofolate |