CatLogenzyme kinetics catalog

Values CatLog read from this paper

Correlations of inhibitor kinetics for Pneumocystis jirovecii and human dihydrofolate reductase with structural data for human active site mutant enzyme complexes

PMID 19196009doi:10.1021/bi801960hOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (40 values) from this paper: dihydrofolate reductase (hDHFR), dihydrofolate reductase (pcDHFR), dihydrofolate reductase (pjDHFR) from Homo sapiens, Pneumocystis carinii, Pneumocystis jirovecii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.5.1.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
dihydrofolate reductase (hDHFR)Homo sapiensdihydrofolic acid400.00271.5 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)Homo sapiensNADPH—0.004—pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant N64FHomo sapiensdihydrofolic acid408.1 × 10⁻⁴4.9 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant N64FHomo sapiensNADPH—0.0052—pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant N64SHomo sapiensdihydrofolic acid471.8 × 10⁻⁴2.61 × 10⁵pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant N64SHomo sapiensNADPH—0.0063—pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensdihydrofolic acid487.6 × 10⁻⁴6.3 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35KHomo sapiensNADPH—0.013—pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensdihydrofolic acid434.9 × 10⁻⁴8.8 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35K/N64FHomo sapiensNADPH—0.0055—pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensdihydrofolic acid350.00113.3 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35SHomo sapiensNADPH—0.0044—pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensdihydrofolic acid270.00161.7 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (hDHFR)variant Q35S/N64SHomo sapiensNADPH—0.0046—pH 7.4, 37 °COpen
dihydrofolate reductase (pcDHFR)Pneumocystis cariniidihydrofolic acid2840.00495.8 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (pcDHFR)Pneumocystis cariniiNADPH—0.0145—pH 7.4, 37 °COpen
dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniidihydrofolic acid360.00854 × 10³pH 7.4, 37 °COpen
dihydrofolate reductase (pcDHFR)variant K37QPneumocystis cariniiNADPH—0.0092—pH 7.4, 37 °COpen
dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciidihydrofolic acid530.00281.9 × 10⁴pH 7.4, 37 °COpen
dihydrofolate reductase (pjDHFR)Pneumocystis jiroveciiNADPH—0.0229—pH 7.4, 37 °COpen