Values CatLog read from this paper
Chimeric dihydrofolate reductases display properties of modularity and biophysical diversity
PMID 31095809doi:10.1002/pro.3646Open in Browse
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CatLog read 7 records (21 values) from this paper: dihydrofolate reductase (DHFR) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrofolate reductase (DHFR) | Escherichia coli | 7,8-dihydrofolate | 13 | 8 × 10⁻⁴ | 1.6 × 10⁴ | pH 7, 25 °C | Open record: dihydrofolate reductase (DHFR), 7,8-dihydrofolate |
| dihydrofolate reductase (DHFR)chiHi (chimera: helix residues 25-34 replaced by the Haemophilus influenza segment PADLAWFRQK) | Escherichia coli | 7,8-dihydrofolate | 10 | 4 × 10⁻⁴ | 2.5 × 10⁴ | pH 7, 25 °C | Open record: dihydrofolate reductase (DHFR) chiHi (chimera: helix residues 25-34 replaced by the Haemophilus influenza segment PADLAWFRQK), 7,8-dihydrofolate |
| dihydrofolate reductase (DHFR)chiHs (chimera: helix residues 25-34 replaced by the Homo sapiens segment RNEFRYFQRM) | Escherichia coli | 7,8-dihydrofolate | 9 | 0.067 | 130 | pH 7, 25 °C | Open record: dihydrofolate reductase (DHFR) chiHs (chimera: helix residues 25-34 replaced by the Homo sapiens segment RNEFRYFQRM), 7,8-dihydrofolate |
| dihydrofolate reductase (DHFR)chiLg (chimera: helix residues 25-34 replaced by the Listeria grayi segment PADLAYFKKQ) | Escherichia coli | 7,8-dihydrofolate | 14 | 7 × 10⁻⁴ | 2.2 × 10⁴ | pH 7, 25 °C | Open record: dihydrofolate reductase (DHFR) chiLg (chimera: helix residues 25-34 replaced by the Listeria grayi segment PADLAYFKKQ), 7,8-dihydrofolate |
| dihydrofolate reductase (DHFR)chiPf (chimera: helix residues 25-34 replaced by the Plasmodium falciparum segment SLDMKYFCAV) | Escherichia coli | 7,8-dihydrofolate | 33 | 0.058 | 500 | pH 7, 25 °C | Open record: dihydrofolate reductase (DHFR) chiPf (chimera: helix residues 25-34 replaced by the Plasmodium falciparum segment SLDMKYFCAV), 7,8-dihydrofolate |
| dihydrofolate reductase (DHFR)chiSc (chimera: helix residues 25-34 replaced by the Saccharomyces cerevisiae segment PSEMKYFRQV) | Escherichia coli | 7,8-dihydrofolate | 29 | 0.0015 | 2.4 × 10⁴ | pH 7, 25 °C | Open record: dihydrofolate reductase (DHFR) chiSc (chimera: helix residues 25-34 replaced by the Saccharomyces cerevisiae segment PSEMKYFRQV), 7,8-dihydrofolate |
| dihydrofolate reductase (DHFR)chiSd (chimera: helix residues 25-34 replaced by the Streptococcus dysgalactiae segment PRELQHFKET) | Escherichia coli | 7,8-dihydrofolate | 37 | 0.0063 | 6 × 10³ | pH 7, 25 °C | Open record: dihydrofolate reductase (DHFR) chiSd (chimera: helix residues 25-34 replaced by the Streptococcus dysgalactiae segment PRELQHFKET), 7,8-dihydrofolate |