Values CatLog read from this paper
Utilisation of 10-formyldihydrofolate as substrate by dihydrofolate reductase (DHFR) and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) tranformylase/IMP cyclohydrolase (PurH) in…
PMID 29799386doi:10.1099/mic.0.000671Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (21 values) from this paper: dihydrofolate reductase (DHFR, EcoDHFR), AICAR transformylase/IMP cyclohydrolase (PurH, EcoPurH) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AICAR transformylase/IMP cyclohydrolase (PurH, EcoPurH) | Escherichia coli | 10-formyl-5,6,7,8-tetrahydrofolate | 4.97 | 0.111 | 43 | pH 7.5 | Open record: AICAR transformylase/IMP cyclohydrolase (PurH, EcoPurH), 10-formyl-5,6,7,8-tetrahydrofolate |
| AICAR transformylase/IMP cyclohydrolase (PurH, EcoPurH) | Escherichia coli | 10-formyl-7,8-dihydrofolate | 7.18 | 0.245 | 28 | pH 7.5 | Open record: AICAR transformylase/IMP cyclohydrolase (PurH, EcoPurH), 10-formyl-7,8-dihydrofolate |
| AICAR transformylase/IMP cyclohydrolase (PurH, EcoPurH) | Escherichia coli | 5-aminoimidazole-4-carboxamide ribonucleotide | 3.63 | 0.0346 | 105 | pH 7.5 | Open record: AICAR transformylase/IMP cyclohydrolase (PurH, EcoPurH), 5-aminoimidazole-4-carboxamide ribonucleotide |
| dihydrofolate reductase (DHFR, EcoDHFR) | Escherichia coli | 10-formyl-7,8-dihydrofolate | 4.58 | 0.003 | 1.53 × 10³ | pH 7.5 | Open record: dihydrofolate reductase (DHFR, EcoDHFR), 10-formyl-7,8-dihydrofolate |
| dihydrofolate reductase (DHFR, EcoDHFR) | Escherichia coli | 7,8-dihydrofolate | 22.83 | 0.0055 | 4.15 × 10³ | pH 7.5 | Open record: dihydrofolate reductase (DHFR, EcoDHFR), 7,8-dihydrofolate |
| dihydrofolate reductase (DHFR, EcoDHFR) | Escherichia coli | NADPHDHF 50 µM fixed; cosubstrate DHF | 28.07 | 0.008 | 3.5 × 10³ | pH 7.5 | Open record: dihydrofolate reductase (DHFR, EcoDHFR), NADPH |
| dihydrofolate reductase (DHFR, EcoDHFR) | Escherichia coli | NADPH10-CHO-DHF 50 µM fixed; cosubstrate 10-CHO-DHF | 4.05 | 0.0074 | 547 | pH 7.5 | Open record: dihydrofolate reductase (DHFR, EcoDHFR), NADPH |