Values CatLog read from this paper
A mechanism of resistance to methotrexate. NADPH but not NADH stimulation of methotrexate binding to dihydrofolate reductase
PMID 6882460doi:10.1016/0006-2952(83)90047-3Open in Browse
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CatLog read 8 records (8 values) from this paper: dihydrofolate reductase from Mus musculus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrofolate reductase | Mus musculus | dihydrofolateno added salt; cosubstrate NADPH | 5 | — | — | pH 7, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Mus musculus | dihydrofolateplus 0.15 M KCl; cosubstrate NADPH | 12.5 | — | — | pH 7, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Mus musculus | dihydrofolateno added salt; cosubstrate NADH | 5 | — | — | pH 7, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Mus musculus | dihydrofolateplus 0.15 M KCl; cosubstrate NADH | 0.52 | — | — | pH 7, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Mus musculus | dihydrofolateno added salt; cosubstrate NADPH | 6.7 | — | — | pH 5, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Mus musculus | dihydrofolateplus 0.15 M KCl; cosubstrate NADPH | 17 | — | — | pH 5, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Mus musculus | dihydrofolateno added salt; cosubstrate NADH | 2.5 | — | — | pH 5, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |
| dihydrofolate reductase | Mus musculus | dihydrofolateplus 0.15 M KCl; cosubstrate NADH | 1.03 | — | — | pH 5, 37 °C | Open record: dihydrofolate reductase, dihydrofolate |