Values CatLog read from this paper
Properties of bacteriophage T4 thymidylate synthase following mutagenic changes in the active site and folate binding region
PMID 2271601doi:10.1021/bi00493a010Open in Browse
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CatLog read 14 records (35 values) from this paper: thymidylate synthase from bacteriophage T4.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidylate synthase | bacteriophage T4 | 2'-deoxyuridine 5'-monophosphate | 11.8 | 0.00273 | 4.32 × 10³ | pH 7, 30 °C | Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase | bacteriophage T4 | 5,10-methylenetetrahydrofolate | — | 0.0499 | 237 | pH 7, 30 °C | Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant C156S | bacteriophage T4 | 2'-deoxyuridine 5'-monophosphate | 0.008 | 0.00492 | 2 | pH 7, 30 °C | Open record: thymidylate synthase variant C156S, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant C156S | bacteriophage T4 | 5,10-methylenetetrahydrofolate | — | 0.0819 | 0.1 | pH 7, 30 °C | Open record: thymidylate synthase variant C156S, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant H157V | bacteriophage T4 | 2'-deoxyuridine 5'-monophosphate | 2.7 | 0.00412 | 655 | pH 7, 30 °C | Open record: thymidylate synthase variant H157V, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant H157V | bacteriophage T4 | 5,10-methylenetetrahydrofolate | — | 0.0752 | 36 | pH 7, 30 °C | Open record: thymidylate synthase variant H157V, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant K48R | bacteriophage T4 | 2'-deoxyuridine 5'-monophosphate | 0.76 | 0.0059 | 128 | pH 7, 30 °C | Open record: thymidylate synthase variant K48R, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant K48R | bacteriophage T4 | 5,10-methylenetetrahydrofolate | — | 0.187 | 4 | pH 7, 30 °C | Open record: thymidylate synthase variant K48R, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant K49R | bacteriophage T4 | 2'-deoxyuridine 5'-monophosphate | 6.65 | 0.00649 | 1.04 × 10³ | pH 7, 30 °C | Open record: thymidylate synthase variant K49R, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant K49R | bacteriophage T4 | 5,10-methylenetetrahydrofolate | — | 0.0779 | 85 | pH 7, 30 °C | Open record: thymidylate synthase variant K49R, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant P155A | bacteriophage T4 | 2'-deoxyuridine 5'-monophosphate | 10.3 | 0.0118 | 873 | pH 7, 30 °C | Open record: thymidylate synthase variant P155A, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant P155A | bacteriophage T4 | 5,10-methylenetetrahydrofolate | — | 0.0991 | 104 | pH 7, 30 °C | Open record: thymidylate synthase variant P155A, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant R137K | bacteriophage T4 | 2'-deoxyuridine 5'-monophosphate | 3.8 | 0.00702 | 541 | pH 7, 30 °C | Open record: thymidylate synthase variant R137K, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R137K | bacteriophage T4 | 5,10-methylenetetrahydrofolate | — | 0.108 | 35 | pH 7, 30 °C | Open record: thymidylate synthase variant R137K, 5,10-methylenetetrahydrofolate |