CatLogenzyme kinetics catalog

Values CatLog read from this paper

Asparagine 229 in thymidylate synthase contributes to, but is not essential for, catalysis

PMID 8378336doi:10.1073/pnas.90.18.8604Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 44 records (89 values) from this paper: thymidylate synthase from Lactobacillus casei.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.1.1.45

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
thymidylate synthaseLactobacillus casei2'-deoxyuridine 5'-monophosphate3.60.005720pH 7.4Open
thymidylate synthaseLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.01360pH 7.4Open
thymidylate synthasevariant N229ALactobacillus casei2'-deoxyuridine 5'-monophosphate0.0015——pH 7.4Open
thymidylate synthasevariant N229CLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.220.01713pH 7.4Open
thymidylate synthasevariant N229CLactobacillus casei2'-deoxyuridine 5'-monophosphate0.23——pH 7.4Open
thymidylate synthasevariant N229CLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0922.4pH 7.4Open
thymidylate synthasevariant N229DLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.00370.050.074pH 7.4Open
thymidylate synthasevariant N229DLactobacillus casei2'-deoxyuridine 5'-monophosphate0.0041——pH 7.4Open
thymidylate synthasevariant N229DLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0350.11pH 7.4Open
thymidylate synthasevariant N229ELactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.00430.0660.065pH 7.4Open
thymidylate synthasevariant N229ELactobacillus casei2'-deoxyuridine 5'-monophosphate0.0045——pH 7.4Open
thymidylate synthasevariant N229ELactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.080.054pH 7.4Open
thymidylate synthasevariant N229GLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.0280.0151.9pH 7.4Open
thymidylate synthasevariant N229GLactobacillus casei2'-deoxyuridine 5'-monophosphate0.03——pH 7.4Open
thymidylate synthasevariant N229GLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0980.29pH 7.4Open
thymidylate synthasevariant N229ILactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit1.10.1497.4pH 7.4Open
thymidylate synthasevariant N229ILactobacillus casei2'-deoxyuridine 5'-monophosphate1.3——pH 7.4Open
thymidylate synthasevariant N229ILactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.08912pH 7.4Open
thymidylate synthasevariant N229KLactobacillus casei2'-deoxyuridine 5'-monophosphate0.00780.00681.1pH 7.4Open
thymidylate synthasevariant N229KLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0190.41pH 7.4Open
thymidylate synthasevariant N229LLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit1.10.2234.9pH 7.4Open
thymidylate synthasevariant N229LLactobacillus casei2'-deoxyuridine 5'-monophosphate1.4——pH 7.4Open
thymidylate synthasevariant N229LLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0912pH 7.4Open
thymidylate synthasevariant N229MLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.620.1544pH 7.4Open
thymidylate synthasevariant N229MLactobacillus casei2'-deoxyuridine 5'-monophosphate0.71——pH 7.4Open
thymidylate synthasevariant N229MLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0847.4pH 7.4Open
thymidylate synthasevariant N229QLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.0170.0111.5pH 7.4Open
thymidylate synthasevariant N229QLactobacillus casei2'-deoxyuridine 5'-monophosphate0.018——pH 7.4Open
thymidylate synthasevariant N229QLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0380.45pH 7.4Open
thymidylate synthasevariant N229SLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.00930.0390.24pH 7.4Open
thymidylate synthasevariant N229SLactobacillus casei2'-deoxyuridine 5'-monophosphate0.011——pH 7.4Open
thymidylate synthasevariant N229SLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0150.62pH 7.4Open
thymidylate synthasevariant N229TLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.0770.1040.74pH 7.4Open
thymidylate synthasevariant N229TLactobacillus casei2'-deoxyuridine 5'-monophosphate0.084——pH 7.4Open
thymidylate synthasevariant N229TLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0661.2pH 7.4Open
thymidylate synthasevariant N229VLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.860.0919.5pH 7.4Open
thymidylate synthasevariant N229VLactobacillus casei2'-deoxyuridine 5'-monophosphate0.95——pH 7.4Open
thymidylate synthasevariant N229VLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.05715pH 7.4Open
thymidylate synthasevariant N229WLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit1.20.5762.1pH 7.4Open
thymidylate synthasevariant N229WLactobacillus casei2'-deoxyuridine 5'-monophosphate1.8——pH 7.4Open
thymidylate synthasevariant N229WLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.1736.9pH 7.4Open
thymidylate synthasevariant N229YLactobacillus casei2'-deoxyuridine 5'-monophosphatenonlinear least-squares fit0.00870.0140.62pH 7.4Open
thymidylate synthasevariant N229YLactobacillus casei2'-deoxyuridine 5'-monophosphate0.0093——pH 7.4Open
thymidylate synthasevariant N229YLactobacillus casei5,10-methylene-5,6,7,8-tetrahydrofolate—0.0090.97pH 7.4Open