Values CatLog read from this paper
A novel dCMP methylase by engineering thymidylate synthase
PMID 9398324doi:10.1021/bi971873hOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (31 values) from this paper: thymidylate synthase (TS) from Lactobacillus casei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidylate synthase (TS) | Lactobacillus casei | 2'-deoxyuridine-5'-monophosphate | 8 | 0.007 | 1.1 × 10³ | pH 7.4 | Open record: thymidylate synthase (TS), 2'-deoxyuridine-5'-monophosphate |
| thymidylate synthase (TS) | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.018 | — | pH 7.4 | Open record: thymidylate synthase (TS), 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase (TS)variant H199A/N229D | Lactobacillus casei | 2'-deoxycytidine 5'-monophosphate | 1.76 | 0.006 | 290 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199A/N229D, 2'-deoxycytidine 5'-monophosphate |
| thymidylate synthase (TS)variant H199A/N229D | Lactobacillus casei | 2'-deoxyuridine-5'-monophosphate | 0.0019 | 0.28 | 0.0068 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199A/N229D, 2'-deoxyuridine-5'-monophosphate |
| thymidylate synthase (TS)variant H199A/N229D | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.029 | 60 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199A/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase (TS)variant H199G/N229D | Lactobacillus casei | 2'-deoxycytidine 5'-monophosphate | 2.54 | 0.012 | 220 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199G/N229D, 2'-deoxycytidine 5'-monophosphate |
| thymidylate synthase (TS)variant H199G/N229D | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.031 | 80 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199G/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase (TS)variant H199N/N229D | Lactobacillus casei | 2'-deoxycytidine 5'-monophosphate | 0.33 | 0.008 | 40 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199N/N229D, 2'-deoxycytidine 5'-monophosphate |
| thymidylate synthase (TS)variant H199N/N229D | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.097 | 3 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199N/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase (TS)variant H199P/N229D | Lactobacillus casei | 2'-deoxyuridine-5'-monophosphate | 0.015 | — | — | pH 7.4 | Open record: thymidylate synthase (TS) variant H199P/N229D, 2'-deoxyuridine-5'-monophosphate |
| thymidylate synthase (TS)variant H199V/N229D | Lactobacillus casei | 2'-deoxycytidine 5'-monophosphate | 1.93 | 0.013 | 150 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199V/N229D, 2'-deoxycytidine 5'-monophosphate |
| thymidylate synthase (TS)variant H199V/N229D | Lactobacillus casei | 2'-deoxyuridine-5'-monophosphate | 7 × 10⁻⁴ | 0.47 | 0.0015 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199V/N229D, 2'-deoxyuridine-5'-monophosphate |
| thymidylate synthase (TS)variant H199V/N229D | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.013 | 150 | pH 7.4 | Open record: thymidylate synthase (TS) variant H199V/N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate |