Values CatLog read from this paper
Replacement set mutagenesis of the four phosphate-binding arginine residues of thymidylate synthase
PMID 10964985doi:10.1093/protein/13.8.557Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 105 records (238 values) from this paper: thymidylate synthase from Lactobacillus casei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 25 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidylate synthase | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.02 | 280 | 25 °C | Open record: thymidylate synthase, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.02 | 360 | 25 °C | Open record: thymidylate synthase, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.02 | 260 | 25 °C | Open record: thymidylate synthase, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthase | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 5.1 | 0.0026 | 2 × 10³ | 25 °C | Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 7.2 | 0.003 | 2.4 × 10³ | 25 °C | Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 5.2 | 0.0028 | 1.9 × 10³ | 25 °C | Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.011 | 0.009 | 1.2 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R178A | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.41 | 5.9 | 25 °C | Open record: thymidylate synthase variant R178A, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R178A | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.4 | 5.5 | 0.44 | 25 °C | Open record: thymidylate synthase variant R178A, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R178C | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.22 | 14.1 | 25 °C | Open record: thymidylate synthase variant R178C, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R178C | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 3.1 | 3.9 | 0.79 | 25 °C | Open record: thymidylate synthase variant R178C, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R178K | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.06 | 133 | 25 °C | Open record: thymidylate synthase variant R178K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R178K | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 8 | 0.13 | 61.54 | 25 °C | Open record: thymidylate synthase variant R178K, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R178S | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.31 | 7.1 | 25 °C | Open record: thymidylate synthase variant R178S, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R178S | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.2 | 4.5 | 0.49 | 25 °C | Open record: thymidylate synthase variant R178S, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179A | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.01 | 480 | 25 °C | Open record: thymidylate synthase variant R179A, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179A | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 4.8 | 0.0068 | 710 | 25 °C | Open record: thymidylate synthase variant R179A, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179A | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0035 | 0.09 | 0.039 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179A, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179C | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.026 | 73 | 25 °C | Open record: thymidylate synthase variant R179C, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179C | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.6 | 0.0055 | 470 | 25 °C | Open record: thymidylate synthase variant R179C, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179C | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.003 | 0.053 | 0.057 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179C, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179D | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.037 | 57 | 25 °C | Open record: thymidylate synthase variant R179D, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179D | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 1.9 | 0.0028 | 680 | 25 °C | Open record: thymidylate synthase variant R179D, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179E | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.013 | 190 | 25 °C | Open record: thymidylate synthase variant R179E, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179E | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.5 | 0.02 | 130 | 25 °C | Open record: thymidylate synthase variant R179E, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179F | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.031 | 32 | 25 °C | Open record: thymidylate synthase variant R179F, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179F | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 1.4 | 0.0027 | 520 | 25 °C | Open record: thymidylate synthase variant R179F, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179F | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 6 × 10⁻⁴ | 0.036 | 0.017 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179F, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179G | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.028 | 140 | 25 °C | Open record: thymidylate synthase variant R179G, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179G | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 3.2 | 0.0042 | 760 | 25 °C | Open record: thymidylate synthase variant R179G, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179G | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 9 × 10⁻⁴ | 0.074 | 0.012 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179G, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179H | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.048 | 40 | 25 °C | Open record: thymidylate synthase variant R179H, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179H | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.6 | 0.0091 | 290 | 25 °C | Open record: thymidylate synthase variant R179H, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179H | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0029 | 0.085 | 0.034 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179H, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179K | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.021 | 150 | 25 °C | Open record: thymidylate synthase variant R179K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179K | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 3.2 | 0.008 | 400 | 25 °C | Open record: thymidylate synthase variant R179K, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179K | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.003 | 0.061 | 0.049 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179K, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179L | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.051 | 33 | 25 °C | Open record: thymidylate synthase variant R179L, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179L | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.3 | 0.0058 | 400 | 25 °C | Open record: thymidylate synthase variant R179L, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179L | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.001 | 0.022 | 0.045 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179L, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179M | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.035 | 51 | 25 °C | Open record: thymidylate synthase variant R179M, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179M | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.5 | 0.0066 | 380 | 25 °C | Open record: thymidylate synthase variant R179M, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179M | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0022 | 0.049 | 0.045 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179M, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179P | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.324 | 0.005 | 25 °C | Open record: thymidylate synthase variant R179P, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179P | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.0016 | 0.098 | 0.02 | 25 °C | Open record: thymidylate synthase variant R179P, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179Q | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.027 | 70 | 25 °C | Open record: thymidylate synthase variant R179Q, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179Q | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.3 | 0.0048 | 480 | 25 °C | Open record: thymidylate synthase variant R179Q, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179Q | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0047 | 0.034 | 0.14 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179Q, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179S | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.019 | 89 | 25 °C | Open record: thymidylate synthase variant R179S, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179S | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.3 | 0.0074 | 310 | 25 °C | Open record: thymidylate synthase variant R179S, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179S | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0015 | 0.028 | 0.054 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179S, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179T | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0016 | 0.021 | 0.076 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179T, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179V | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.042 | 33 | 25 °C | Open record: thymidylate synthase variant R179V, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179V | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 1.9 | 0.0063 | 300 | 25 °C | Open record: thymidylate synthase variant R179V, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179V | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0013 | 0.035 | 0.037 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179V, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179W | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.058 | 48 | 25 °C | Open record: thymidylate synthase variant R179W, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R179W | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 2.6 | 0.0055 | 470 | 25 °C | Open record: thymidylate synthase variant R179W, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R179W | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.0019 | 0.042 | 0.045 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R179W, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218A | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.005 | — | — | 25 °C | Open record: thymidylate synthase variant R218A, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218E | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 1.4 × 10⁻⁴ | — | — | 25 °C | Open record: thymidylate synthase variant R218E, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218H | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 4.5 × 10⁻⁴ | — | — | 25 °C | Open record: thymidylate synthase variant R218H, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218K | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.096 | 1.9 | 25 °C | Open record: thymidylate synthase variant R218K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R218K | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.18 | 0.3 | 6 | 25 °C | Open record: thymidylate synthase variant R218K, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218L | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.001 | — | — | 25 °C | Open record: thymidylate synthase variant R218L, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218Q | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.0021 | — | — | 25 °C | Open record: thymidylate synthase variant R218Q, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218S | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 7.1 × 10⁻⁴ | — | — | 25 °C | Open record: thymidylate synthase variant R218S, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218V | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 8.5 × 10⁻⁴ | — | — | 25 °C | Open record: thymidylate synthase variant R218V, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R218Y | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 7.4 × 10⁻⁴ | — | — | 25 °C | Open record: thymidylate synthase variant R218Y, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23A | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.558 | 0.4 | 25 °C | Open record: thymidylate synthase variant R23A, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23A | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.21 | 0.052 | 4 | 25 °C | Open record: thymidylate synthase variant R23A, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23A | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.007 | 0.0053 | 1.3 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23A, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23D | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.31 | 0.4 | 25 °C | Open record: thymidylate synthase variant R23D, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23D | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.12 | 0.009 | 13 | 25 °C | Open record: thymidylate synthase variant R23D, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23D | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.007 | 0.012 | 0.58 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23D, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23E | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.152 | 1.6 | 25 °C | Open record: thymidylate synthase variant R23E, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23E | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.25 | 0.051 | 4.9 | 25 °C | Open record: thymidylate synthase variant R23E, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23E | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.007 | 0.016 | 0.44 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23E, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23F | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.444 | 0.3 | 25 °C | Open record: thymidylate synthase variant R23F, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23F | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.12 | 0.029 | 4.1 | 25 °C | Open record: thymidylate synthase variant R23F, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23G | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.473 | 0.3 | 25 °C | Open record: thymidylate synthase variant R23G, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23G | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.12 | 0.027 | 4.4 | 25 °C | Open record: thymidylate synthase variant R23G, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23G | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.009 | 0.018 | 0.50 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23G, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23I | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.007 | 0.038 | 0.18 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23I, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23K | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.358 | 0.4 | 25 °C | Open record: thymidylate synthase variant R23K, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23K | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.16 | 0.02 | 8 | 25 °C | Open record: thymidylate synthase variant R23K, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23K | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.011 | 0.011 | 1.0 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23K, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23L | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.786 | 0.1 | 25 °C | Open record: thymidylate synthase variant R23L, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23L | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.11 | 0.027 | 4.1 | 25 °C | Open record: thymidylate synthase variant R23L, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23L | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.006 | 0.0047 | 1.3 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23L, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23N | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.387 | 0.2 | 25 °C | Open record: thymidylate synthase variant R23N, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23N | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.06 | 0.02 | 3 | 25 °C | Open record: thymidylate synthase variant R23N, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23N | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.01 | 0.011 | 0.91 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23N, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23P | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.79 | 0.1 | 25 °C | Open record: thymidylate synthase variant R23P, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23P | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.04 | 0.035 | 1.1 | 25 °C | Open record: thymidylate synthase variant R23P, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23Q | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.221 | 2.4 | 25 °C | Open record: thymidylate synthase variant R23Q, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23Q | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.53 | 0.022 | 24 | 25 °C | Open record: thymidylate synthase variant R23Q, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23S | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.284 | 0.7 | 25 °C | Open record: thymidylate synthase variant R23S, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23S | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.2 | 0.019 | 11 | 25 °C | Open record: thymidylate synthase variant R23S, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23S | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.01 | 0.016 | 0.62 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23S, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23V | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.422 | 0.6 | 25 °C | Open record: thymidylate synthase variant R23V, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23V | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.27 | 0.032 | 8.4 | 25 °C | Open record: thymidylate synthase variant R23V, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23V | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.007 | 0.024 | 0.29 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23V, 5-bromo-2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23Y | Lactobacillus casei | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.423 | 0.4 | 25 °C | Open record: thymidylate synthase variant R23Y, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant R23Y | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.15 | 0.005 | 30 | 25 °C | Open record: thymidylate synthase variant R23Y, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant R23Y | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.01 | 0.019 | 0.53 calc. | pH 7.4, 25 °C | Open record: thymidylate synthase variant R23Y, 5-bromo-2'-deoxyuridine 5'-monophosphate |