Values CatLog read from this paper
Mutation of asparagine 229 to aspartate in thymidylate synthase converts the enzyme to a deoxycytidylate methylase
PMID 1606134doi:10.1021/bi00137a002Open in Browse
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CatLog read 6 records (15 values) from this paper: thymidylate synthase from Lactobacillus casei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidylate synthase | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 3.6 | 0.005 | 710 | pH 7.4, 20 °C | Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolate | — | 0.01 | 360 | pH 7.4, 20 °C | Open record: thymidylate synthase, 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant N229D | Lactobacillus casei | 2'-deoxycytidine 5'-monophosphate | 0.45 | 0.15 | 3 | pH 7.4, 20 °C | Open record: thymidylate synthase variant N229D, 2'-deoxycytidine 5'-monophosphate |
| thymidylate synthasevariant N229D | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | 0.0037 | 0.0504 | 0.073 | pH 7.4, 20 °C | Open record: thymidylate synthase variant N229D, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant N229D | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolateTS reaction (dTMP formation); cosubstrate dUMP | — | 0.0354 | 0.01 | pH 7.4, 20 °C | Open record: thymidylate synthase variant N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate |
| thymidylate synthasevariant N229D | Lactobacillus casei | 5,10-methylene-5,6,7,8-tetrahydrofolatedCMP methylase reaction (m5dCMP formation); cosubstrate dCMP | — | 0.17 | 2.6 | pH 7.4, 20 °C | Open record: thymidylate synthase variant N229D, 5,10-methylene-5,6,7,8-tetrahydrofolate |