Values CatLog read from this paper
Complete replacement set of amino acids at the C-terminus of thymidylate synthase: quantitative structure-activity relationship of mutants of an enzyme
PMID 1627545doi:10.1021/bi00141a011Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 46 records (111 values) from this paper: thymidylate synthase from Lactobacillus casei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymidylate synthase | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0029 | 1.9 × 10³ | 25 °C | Open record: thymidylate synthase, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthase | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 5.5 | 0.014 | 400 | 25 °C | Open record: thymidylate synthase, 5,10-methylenetetrahydrofolate |
| thymidylate synthase | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.013 | 0.012 | 1.1 calc. | Open record: thymidylate synthase, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316A | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0012 | 690 | 25 °C | Open record: thymidylate synthase variant V316A, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316A | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.81 | 0.37 | 2.2 | 25 °C | Open record: thymidylate synthase variant V316A, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316A | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.015 | 0.024 | 0.62 calc. | Open record: thymidylate synthase variant V316A, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316C | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0016 | 720 | 25 °C | Open record: thymidylate synthase variant V316C, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316C | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 1.1 | 0.077 | 15 | 25 °C | Open record: thymidylate synthase variant V316C, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316E | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0025 | 60 | 25 °C | Open record: thymidylate synthase variant V316E, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316E | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.15 | 0.83 | 0.18 | 25 °C | Open record: thymidylate synthase variant V316E, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316E | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.01 | 0.022 | 0.45 calc. | Open record: thymidylate synthase variant V316E, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316F | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0022 | 590 | 25 °C | Open record: thymidylate synthase variant V316F, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316F | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 1.3 | 0.065 | 20 | 25 °C | Open record: thymidylate synthase variant V316F, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316G | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0056 | 5.4 | 25 °C | Open record: thymidylate synthase variant V316G, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316G | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.03 | 0.38 | 0.078 | 25 °C | Open record: thymidylate synthase variant V316G, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316G | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.008 | 0.01 | 0.80 calc. | Open record: thymidylate synthase variant V316G, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316H | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0016 | 340 | 25 °C | Open record: thymidylate synthase variant V316H, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316H | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.55 | 0.05 | 11 | 25 °C | Open record: thymidylate synthase variant V316H, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316I | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0022 | 1.7 × 10³ | 25 °C | Open record: thymidylate synthase variant V316I, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316I | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 3.8 | 0.035 | 110 | 25 °C | Open record: thymidylate synthase variant V316I, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316K | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0012 | 99 | 25 °C | Open record: thymidylate synthase variant V316K, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316K | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.12 | 0.085 | 1.4 | 25 °C | Open record: thymidylate synthase variant V316K, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316K | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.013 | 0.016 | 0.81 calc. | Open record: thymidylate synthase variant V316K, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316L | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0017 | 810 | 25 °C | Open record: thymidylate synthase variant V316L, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316L | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 1.3 | 0.084 | 16 | 25 °C | Open record: thymidylate synthase variant V316L, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316M | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0025 | 260 | 25 °C | Open record: thymidylate synthase variant V316M, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316M | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.65 | 0.12 | 5.4 | 25 °C | Open record: thymidylate synthase variant V316M, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316N | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0014 | 270 | 25 °C | Open record: thymidylate synthase variant V316N, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316N | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.39 | 0.17 | 2.3 | 25 °C | Open record: thymidylate synthase variant V316N, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316P | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0042 | 140 | 25 °C | Open record: thymidylate synthase variant V316P, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316P | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.6 | 0.57 | 1.1 | 25 °C | Open record: thymidylate synthase variant V316P, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316Q | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0031 | 100 | 25 °C | Open record: thymidylate synthase variant V316Q, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316Q | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.32 | 0.28 | 1.1 | 25 °C | Open record: thymidylate synthase variant V316Q, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316Q | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.013 | 0.015 | 0.87 calc. | Open record: thymidylate synthase variant V316Q, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316R | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0015 | 14 | 25 °C | Open record: thymidylate synthase variant V316R, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316R | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.02 | 0.13 | 0.15 | 25 °C | Open record: thymidylate synthase variant V316R, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316S | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0017 | 320 | 25 °C | Open record: thymidylate synthase variant V316S, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316S | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.54 | 0.18 | 3 | 25 °C | Open record: thymidylate synthase variant V316S, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316S | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.008 | 0.012 | 0.67 calc. | Open record: thymidylate synthase variant V316S, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316T | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0035 | 340 | 25 °C | Open record: thymidylate synthase variant V316T, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316T | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 1.2 | 0.14 | 8.1 | 25 °C | Open record: thymidylate synthase variant V316T, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316T | Lactobacillus casei | 5-bromo-2'-deoxyuridine 5'-monophosphate | 0.01 | 0.015 | 0.67 calc. | Open record: thymidylate synthase variant V316T, 5-bromo-2'-deoxyuridine 5'-monophosphate | |
| thymidylate synthasevariant V316W | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0015 | 34 | 25 °C | Open record: thymidylate synthase variant V316W, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316W | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.05 | 0.3 | 0.17 | 25 °C | Open record: thymidylate synthase variant V316W, 5,10-methylenetetrahydrofolate |
| thymidylate synthasevariant V316Y | Lactobacillus casei | 2'-deoxyuridine 5'-monophosphate | — | 0.0024 | 120 | 25 °C | Open record: thymidylate synthase variant V316Y, 2'-deoxyuridine 5'-monophosphate |
| thymidylate synthasevariant V316Y | Lactobacillus casei | 5,10-methylenetetrahydrofolate | 0.29 | 0.17 | 1.7 | 25 °C | Open record: thymidylate synthase variant V316Y, 5,10-methylenetetrahydrofolate |