Values CatLog read from this paper
Regulation of protein kinase D by multisite phosphorylation. Identification of phosphorylation sites by mass spectrometry and characterization by site-directed mutagenesis
PMID 10867018doi:10.1074/jbc.m001357200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 41 records (61 values) from this paper: protein kinase D (PKD/PKCmu).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 20 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| protein kinase D (PKD/PKCmu) | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.074 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu) | — | Mg2+-ATP complexfull-length PKD with N-terminal GST tag (GST-PKD) | — | 0.058 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu) | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.065 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu) | — | Mg2+-ATP complex | — | 0.083 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu) | — | syntide-2unstimulated HEK-293T cells (basal) | 0.45 | 0.036 | 12 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), syntide-2 |
| protein kinase D (PKD/PKCmu) | — | syntide-2full-length PKD with N-terminal GST tag (GST-PKD) | 1.2 | 0.026 | 46 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), syntide-2 |
| protein kinase D (PKD/PKCmu) | — | syntide-2unstimulated HEK-293T cells (basal) | 5.3 | 0.048 | 110 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), syntide-2 |
| protein kinase D (PKD/PKCmu) | — | syntide-2 | 6.1 | 0.068 | 90 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu), syntide-2 |
| protein kinase D (PKD/PKCmu)variant S255A | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.088 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S255A | — | Mg2+-ATP complex | — | 0.057 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S255A | — | syntide-2unstimulated HEK-293T cells (basal) | 0.12 | 0.021 | 5.7 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S255A | — | syntide-2 | 0.8 | 0.019 | 42 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S255E | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.095 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S255E | — | Mg2+-ATP complex | — | 0.074 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S255E | — | syntide-2unstimulated HEK-293T cells (basal) | 0.23 | 0.022 | 10 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255E, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S255E | — | syntide-2 | 2.6 | 0.037 | 70 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S255E, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S744A | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.058 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S744A | — | Mg2+-ATP complex | — | 0.14 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S744A | — | syntide-2unstimulated HEK-293T cells (basal) | 0.02 | 0.19 | 0.11 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S744A | — | syntide-2 | 0.09 | 0.047 | 1.9 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S744A | — | syntide-2 | 0.25 | — | — | Open record: protein kinase D (PKD/PKCmu) variant S744A, syntide-2 | |
| protein kinase D (PKD/PKCmu)variant S744E | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.149 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S744E | — | Mg2+-ATP complex | — | 0.083 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S744E | — | syntide-2unstimulated HEK-293T cells (basal) | 0.11 | 0.047 | 2.3 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744E, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S744E | — | syntide-2 | 2.7 | 0.042 | 64 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S744E, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S748A | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.052 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S748A | — | Mg2+-ATP complex | — | 0.098 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S748A | — | syntide-2unstimulated HEK-293T cells (basal) | 0.06 | 0.081 | 0.74 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S748A | — | syntide-2 | 0.13 | 0.06 | 2.2 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S748E | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.063 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S748E | — | Mg2+-ATP complex | — | 0.083 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S748E | — | syntide-2unstimulated HEK-293T cells (basal) | 0.45 | 0.047 | 9.6 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748E, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S748E | — | syntide-2 | 3.4 | 0.071 | 48 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S748E, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S916A | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.062 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S916A | — | Mg2+-ATP complex | — | 0.055 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916A, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S916A | — | syntide-2unstimulated HEK-293T cells (basal) | 0.33 | 0.028 | 12 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S916A | — | syntide-2 | 1 | 0.025 | 40 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916A, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S916E | — | Mg2+-ATP complexunstimulated HEK-293T cells (basal) | — | 0.087 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S916E | — | Mg2+-ATP complex | — | 0.059 | — | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916E, Mg2+-ATP complex |
| protein kinase D (PKD/PKCmu)variant S916E | — | syntide-2unstimulated HEK-293T cells (basal) | 0.66 | 0.025 | 26 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916E, syntide-2 |
| protein kinase D (PKD/PKCmu)variant S916E | — | syntide-2 | 1.9 | 0.063 | 30 calc. | pH 8, 30 °C | Open record: protein kinase D (PKD/PKCmu) variant S916E, syntide-2 |