CatLogenzyme kinetics catalog

EC 2.7.11.1: non-specific serine/threonine protein kinase

TransferasesOpen in Browse

CatLog read 111 records for EC 2.7.11.1 from 7 papers, for 3 organisms. Names in these papers: protein kinase D (PKD/PKCmu), protein kinase CK1α (casein kinase 1 alpha), phosphoglycerate kinase, phosphoglycerate kinase 1 and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 130 records for EC 2.7.11.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.11.1 in Browse.

Papers

PaperOrganismsRecords
Regulation of protein kinase D by multisite phosphorylation. Identification of phosphorylation sites by mass spectrometry and characterization by site-directed mutagenesis—41
Generation of protein kinase Ck1alpha mutants which discriminate between canonical and non-canonical substratesDanio rerio37
Kinetic mechanism of AKT/PKB enzyme family—12
The fast-reacting sulfhydryl group of rat muscle phosphoglycerate kinase is necessary for activity and maintenance of tertiary structureRattus norvegicus9
The phosphoglycerate kinase 1 variants found in carcinoma cells display different catalytic activity and conformational stability compared to the native enzymeHomo sapiens8
Molecular basis for the substrate specificity of protein kinase B; comparison with MAPKAP kinase-1 and p70 S6 kinaseRattus norvegicus3
A selective IKK-2 inhibitor blocks NF-kappa B-dependent gene expression in interleukin-1 beta-stimulated synovial fibroblastsHomo sapiens1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
AKT1 (PKBalpha)—adenosine 5'-triphosphatesequential mechanism fit; purified enzyme activated in vitro0.370.05286.9 calc.pH 7.4PMID 16540465Open
AKT1 (PKBalpha)—adenosine 5'-triphosphate—0.1433—pH 7.4PMID 16540465Open
AKT1 (PKBalpha)—ARKRERTYSFGHHAsequential mechanism fit; purified enzyme activated in vitro—5 × 10⁻⁴—pH 7.4PMID 16540465Open
AKT1 (PKBalpha)—ARKRERTYSFGHHA—0.0029—pH 7.4PMID 16540465Open
AKT2 (PKBbeta)—adenosine 5'-triphosphatesequential mechanism fit; purified enzyme activated in vitro0.420.35841.2 calc.pH 7.4PMID 16540465Open
AKT2 (PKBbeta)—adenosine 5'-triphosphate—0.564—pH 7.4PMID 16540465Open
AKT2 (PKBbeta)—ARKRERTYSFGHHAsequential mechanism fit; purified enzyme activated in vitro—0.0034—pH 7.4PMID 16540465Open
AKT2 (PKBbeta)—ARKRERTYSFGHHA—0.0023—pH 7.4PMID 16540465Open
AKT3 (PKBgamma)—adenosine 5'-triphosphatesequential mechanism fit; purified enzyme activated in vitro1.980.087922.6 calc.pH 7.4PMID 16540465Open
AKT3 (PKBgamma)—adenosine 5'-triphosphate—0.1187—pH 7.4PMID 16540465Open
AKT3 (PKBgamma)—ARKRERTYSFGHHAsequential mechanism fit; purified enzyme activated in vitro—0.0124—pH 7.4PMID 16540465Open
AKT3 (PKBgamma)—ARKRERTYSFGHHA—0.0023—pH 7.4PMID 16540465Open
p70 S6 kinaseRattus norvegicusArg-Pro-Arg-Thr-Ser-Ser-Phe—0.125—PMID 8985174Open
p70 S6 kinaseRattus norvegicusGRPRTSSFAEG (Crosstide)—0.003—PMID 8985174Open
p70 S6 kinaseRattus norvegicusRPRTSTF—0.211—PMID 8985174Open
phosphoglycerate kinaseRattus norvegicus1,3-diphosphoglycerate25.420.042610 calc.pH 6.9, 30 °CPMID 7159552Open
phosphoglycerate kinaseRattus norvegicusD-glycerate 3-phosphate3.982.221.79 calc.pH 7.3, 30 °CPMID 7159552Open
phosphoglycerate kinaseRattus norvegicusMgADP33.130.9535 calc.pH 6.9, 30 °CPMID 7159552Open
phosphoglycerate kinaseRattus norvegicusMgADP—2.75—PMID 7159552Open
phosphoglycerate kinaseRattus norvegicusMgATP8.650.8310 calc.pH 7.3, 30 °CPMID 7159552Open
phosphoglycerate kinasevariant iodoacetate-modified (partially inactivated, carboxymethylated fast-reacting Cys)Rattus norvegicus1,3-diphosphoglycerate25.68——pH 6.9, 30 °CPMID 7159552Open
phosphoglycerate kinasevariant iodoacetate-modified (partially inactivated, carboxymethylated fast-reacting Cys)Rattus norvegicusD-glycerate 3-phosphate3.932.721.45 calc.pH 7.3, 30 °CPMID 7159552Open
phosphoglycerate kinasevariant iodoacetate-modified (partially inactivated, carboxymethylated fast-reacting Cys)Rattus norvegicusMgADP32.87133 calc.pH 6.9, 30 °CPMID 7159552Open
phosphoglycerate kinasevariant iodoacetate-modified (partially inactivated, carboxymethylated fast-reacting Cys)Rattus norvegicusMgATP9.130.9110 calc.pH 7.3, 30 °CPMID 7159552Open
phosphoglycerate kinase 1Homo sapiens3-phosphoglycerate89.8——pH 8, 20 °CPMID 29995887Open
phosphoglycerate kinase 1variant A199VHomo sapiens3-phosphoglycerate76.61.9539.3pH 8, 20 °CPMID 29995887Open
phosphoglycerate kinase 1variant F241SHomo sapiens3-phosphoglycerate213——pH 8, 20 °CPMID 29995887Open
phosphoglycerate kinase 1variant G166DHomo sapiens3-phosphoglycerate5.91.543.8pH 8, 20 °CPMID 29995887Open
phosphoglycerate kinase 1variant M189IHomo sapiens3-phosphoglycerate30.5——pH 8, 20 °CPMID 29995887Open
phosphoglycerate kinase 1variant R38MHomo sapiens3-phosphoglycerate7.2 × 10⁻⁶3.152.28 × 10⁻⁶pH 8, 20 °CPMID 29995887Open
phosphoglycerate kinase 1variant R65WHomo sapiens3-phosphoglycerate23.71.6114.7pH 8, 20 °CPMID 29995887Open
phosphoglycerate kinase 1variant V216FHomo sapiens3-phosphoglycerate1.90.782.4pH 8, 20 °CPMID 29995887Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioadenosine 5'-triphosphate—0.01—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioRRKHAAIGADDDAYSITA—0.168—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioRRKHAAIGDADDAYSITA—0.19—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioRRKHAAIGDDADAYSITA—0.146—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioRRKHAAIGDDDAAYSITA—0.33—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioRRKHAAIGDDDDAYSITA—0.318—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioRRKHAAIGpSAYSITA (pS = phosphoserine)—0.03—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)Danio rerioRRKHAAIpSAAYSITA (pS = phosphoserine)—0.032—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229ADanio rerioRRKHAAIGADDDAYSITA—1.622—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229ADanio rerioRRKHAAIGDADDAYSITA—2.307—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229ADanio rerioRRKHAAIGDDADAYSITA—0.386—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229ADanio rerioRRKHAAIGDDDAAYSITA—1.245—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229ADanio rerioRRKHAAIGDDDDAYSITA—1.127—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229ADanio rerioRRKHAAIGpSAYSITA (pS = phosphoserine)—0.057—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229ADanio rerioRRKHAAIpSAAYSITA (pS = phosphoserine)—0.104—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioadenosine 5'-triphosphate—0.01—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioRRKHAAIGADDDAYSITA—3.033—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioRRKHAAIGDADDAYSITA—3.782—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioRRKHAAIGDDADAYSITA—0.281—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioRRKHAAIGDDDAAYSITA—0.753—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioRRKHAAIGDDDDAYSITA—2.697—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioRRKHAAIGpSAYSITA (pS = phosphoserine)—0.15—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K229A/K230A/K232ADanio rerioRRKHAAIpSAAYSITA (pS = phosphoserine)—1.35—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K230ADanio rerioRRKHAAIGADDDAYSITA—1.382—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K230ADanio rerioRRKHAAIGDADDAYSITA—3.552—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K230ADanio rerioRRKHAAIGDDADAYSITA—0.169—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K230ADanio rerioRRKHAAIGDDDAAYSITA—4.24—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K230ADanio rerioRRKHAAIGDDDDAYSITA—0.967—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K230ADanio rerioRRKHAAIGpSAYSITA (pS = phosphoserine)—0.074—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K230ADanio rerioRRKHAAIpSAAYSITA (pS = phosphoserine)—0.14—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K232ADanio rerioRRKHAAIGADDDAYSITA—3.005—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K232ADanio rerioRRKHAAIGDADDAYSITA—5.235—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K232ADanio rerioRRKHAAIGDDADAYSITA—0.656—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K232ADanio rerioRRKHAAIGDDDAAYSITA—3.629—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K232ADanio rerioRRKHAAIGDDDDAYSITA—4.5—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K232ADanio rerioRRKHAAIGpSAYSITA (pS = phosphoserine)—0.18—pH 7.5, 37 °CPMID 15975091Open
protein kinase CK1α (casein kinase 1 alpha)variant K232ADanio rerioRRKHAAIpSAAYSITA (pS = phosphoserine)—1.218—pH 7.5, 37 °CPMID 15975091Open
protein kinase D (PKD/PKCmu)—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.074—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)—Mg2+-ATP complexfull-length PKD with N-terminal GST tag (GST-PKD)—0.058—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.065—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)—Mg2+-ATP complex—0.083—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)—syntide-2unstimulated HEK-293T cells (basal)0.450.03612 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)—syntide-2full-length PKD with N-terminal GST tag (GST-PKD)1.20.02646 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)—syntide-2unstimulated HEK-293T cells (basal)5.30.048110 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)—syntide-26.10.06890 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255A—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.088—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255A—Mg2+-ATP complex—0.057—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255A—syntide-2unstimulated HEK-293T cells (basal)0.120.0215.7 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255A—syntide-20.80.01942 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255E—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.095—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255E—Mg2+-ATP complex—0.074—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255E—syntide-2unstimulated HEK-293T cells (basal)0.230.02210 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S255E—syntide-22.60.03770 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744A—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.058—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744A—Mg2+-ATP complex—0.14—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744A—syntide-2unstimulated HEK-293T cells (basal)0.020.190.11 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744A—syntide-20.090.0471.9 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744A—syntide-20.25——PMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744E—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.149—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744E—Mg2+-ATP complex—0.083—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744E—syntide-2unstimulated HEK-293T cells (basal)0.110.0472.3 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S744E—syntide-22.70.04264 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748A—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.052—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748A—Mg2+-ATP complex—0.098—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748A—syntide-2unstimulated HEK-293T cells (basal)0.060.0810.74 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748A—syntide-20.130.062.2 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748E—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.063—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748E—Mg2+-ATP complex—0.083—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748E—syntide-2unstimulated HEK-293T cells (basal)0.450.0479.6 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S748E—syntide-23.40.07148 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916A—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.062—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916A—Mg2+-ATP complex—0.055—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916A—syntide-2unstimulated HEK-293T cells (basal)0.330.02812 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916A—syntide-210.02540 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916E—Mg2+-ATP complexunstimulated HEK-293T cells (basal)—0.087—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916E—Mg2+-ATP complex—0.059—pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916E—syntide-2unstimulated HEK-293T cells (basal)0.660.02526 calc.pH 8, 30 °CPMID 10867018Open
protein kinase D (PKD/PKCmu)variant S916E—syntide-21.90.06330 calc.pH 8, 30 °CPMID 10867018Open
TANK-binding kinase 1 (TBK-1)Homo sapiensp65 peptide 6 (522-540, S529A/S535A): GLPNGLLAGDEDFASIADM1.8520.040545.825 °CPMID 12813046Open