Values CatLog read from this paper
A mutant Escherichia coli tyrosyl-tRNA synthetase utilizes the unnatural amino acid azatyrosine more efficiently than tyrosine
PMID 11006270doi:10.1074/jbc.m003696200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (8 values) from this paper: tyrosyl-tRNA synthetase (TyrRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tyrosyl-tRNA synthetase (TyrRS) | Escherichia coli | L-beta-(5-hydroxy-2-pyridyl)-alanine | 0.11 | 0.0177 | 6.2 calc. | pH 7.5, 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS), L-beta-(5-hydroxy-2-pyridyl)-alanine |
| tyrosyl-tRNA synthetase (TyrRS) | Escherichia coli | L-tyrosine | 0.74 | 0.0033 | 220 calc. | pH 7.5, 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS), L-tyrosine |
| tyrosyl-tRNA synthetase (TyrRS)variant F130S | Escherichia coli | L-beta-(5-hydroxy-2-pyridyl)-alanine | 0.042 | 0.0376 | 1.1 calc. | pH 7.5, 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant F130S, L-beta-(5-hydroxy-2-pyridyl)-alanine |
| tyrosyl-tRNA synthetase (TyrRS)variant F130S | Escherichia coli | L-tyrosine | 1.4 | 0.0661 | 21 calc. | pH 7.5, 30 °C | Open record: tyrosyl-tRNA synthetase (TyrRS) variant F130S, L-tyrosine |