Values CatLog read from this paper
Effect of site-directed mutagenesis of the conserved aspartate and glutamate on E. coli undecaprenyl pyrophosphate synthase catalysis
PMID 11076526doi:10.1021/bi001226hOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (15 values) from this paper: undecaprenyl pyrophosphate synthase (UPPs) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| undecaprenyl pyrophosphate synthase (UPPs)variant D150A | Escherichia coli | isopentenyl pyrophosphate | 1.1 | 0.207 | 5.3 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant D150A, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant D150A | Escherichia coli | trans,trans-farnesyl pyrophosphate | — | 4 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant D150A, trans,trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant D218A | Escherichia coli | isopentenyl pyrophosphate | 0.22 | 0.0044 | 50 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant D218A, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant D218A | Escherichia coli | trans,trans-farnesyl pyrophosphate | — | 4 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant D218A, trans,trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant D26A | Escherichia coli | isopentenyl pyrophosphate | 0.0033 | 0.0141 | 0.23 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant D26A, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant D26A | Escherichia coli | trans,trans-farnesyl pyrophosphate | — | 5 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant D26A, trans,trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant E213A | Escherichia coli | isopentenyl pyrophosphate | 0.026 | 0.28 | 0.093 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant E213A, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant E213A | Escherichia coli | trans,trans-farnesyl pyrophosphate | — | 7 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant E213A, trans,trans-farnesyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant E73A | Escherichia coli | isopentenyl pyrophosphate | 0.3 | 0.0162 | 19 calc. | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant E73A, isopentenyl pyrophosphate |
| undecaprenyl pyrophosphate synthase (UPPs)variant E73A | Escherichia coli | trans,trans-farnesyl pyrophosphate | — | 4 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: undecaprenyl pyrophosphate synthase (UPPs) variant E73A, trans,trans-farnesyl pyrophosphate |