Values CatLog read from this paper
Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars
PMID 11094340doi:10.1016/s1074-5521(00)00035-1Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 283.8 | 0.35 | 811 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxygluconate | 4.6 | 284.9 | 0.016 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant E9K/V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 6 | 54.7 | 0.11 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant E9K/V118A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant G40A/V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 6.6 | 64.8 | 0.1 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant G40A/V118A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 92.8 | 0.15 | 619 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F | Escherichia coli | D-2-keto-3-deoxygluconate | 4.1 | 25 | 0.16 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 51.5 | 0.03 | 1.71 × 10³ | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 8.3 | 14.3 | 0.58 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/E138V | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 2.5 | 0.03 | 93 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/E138V, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/E138V | Escherichia coli | D-2-keto-3-deoxygluconate | 13.9 | 12.8 | 1.09 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/E138V, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/G141S/T105I | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 11.2 | 0.46 | 24 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/G141S/T105I, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/G141S/T105I | Escherichia coli | D-2-keto-3-deoxygluconate | 5.2 | 12.1 | 0.43 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/G141S/T105I, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/T161A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 7.8 | 0.006 | 1.39 × 10³ | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/T161A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/T161A | Escherichia coli | D-2-keto-3-deoxygluconate | 0.05 | 2.4 | 0.021 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/T161A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant V118A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 108.3 | 0.21 | 516 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant V118A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 6.1 | 48.7 | 0.13 | pH 7.5, 25 °C | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant V118A, D-2-keto-3-deoxygluconate |