EC 4.1.2.14: 2-dehydro-3-deoxy-phosphogluconate aldolase
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CatLog read 51 records for EC 4.1.2.14 from 3 papers, for 1 organism. Names in these papers: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 14 records for EC 4.1.2.14 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.2.14 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates | Escherichia coli | 19 |
| Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars | Escherichia coli | 16 |
| Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates | Escherichia coli | 16 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 1 × 10⁻⁵ | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 11 | 32 | 0.33 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 2 | 150 | 0.014 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxy-6-phosphogalactonate | 0.0063 | 0.1 | 0.061 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 83 | 0.1 | 830 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxygluconate | 0.019 | 1.9 | 0.0097 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 27 | 3 | 9 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 2.6 | 56 | 0.047 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 97 | 0.16 | 610 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | D-2-keto-3-deoxygluconate | 0.04 | 2.3 | 0.017 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 6 | 3 | 2 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 1.8 | 51 | 0.035 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | D-2-keto-3-deoxy-6-phosphogalactonate | 2.9 × 10⁻⁴ | 1.2 | 2.4 × 10⁻⁴ | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, D-2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 3.2 | 1.2 | 2.7 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | D-2-keto-3-deoxygluconate | 0.11 | 1.8 | 0.062 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 19 | 5.7 | 3.3 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 40 | 1.7 | 24 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | 2-keto-4-hydroxyoctonoate | 1.5 | 25 | 0.06 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, 2-keto-4-hydroxyoctonoate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 40 | 3 | 13.3 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 2.9 | 26 | 0.11 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | D-2-keto-3-deoxy-6-phosphogalactonate | 0.0026 | 0.95 | 0.0027 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, D-2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 74 | 0.35 | 210 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | D-2-keto-3-deoxygluconate | 0.08 | 1.5 | 0.053 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 175 | 43 | 4.1 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-3-deoxy-6-phosphogalactonate | 0.23 | 0.3 | 0.77 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 27 | 0.05 | 550 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-4-hydroxyoctonoate | 1.7 | 15 | 0.113 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-4-hydroxyoctonoate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 1.7 × 10⁻⁵ | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 212 | 3.5 | 61 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 31 | 0.69 | 45 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 3 × 10⁻⁵ | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 240 | 1.6 | 150 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-3-deoxy-6-phosphogalactonate | 0.006 | 1 | 0.0065 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 41 | 0.24 | 170 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-4-hydroxyoctonoate | 3.1 | 4.3 | 0.72 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-4-hydroxyoctonoate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 283.8 | 0.35 | 811 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxygluconate | 4.6 | 284.9 | 0.016 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant E9K/V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 6 | 54.7 | 0.11 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant E9K/V118A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant G40A/V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 6.6 | 64.8 | 0.1 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant G40A/V118A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 92.8 | 0.15 | 619 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F | Escherichia coli | D-2-keto-3-deoxygluconate | 4.1 | 25 | 0.16 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 51.5 | 0.03 | 1.71 × 10³ | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 8.3 | 14.3 | 0.58 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/E138V | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 2.5 | 0.03 | 93 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/E138V, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/E138V | Escherichia coli | D-2-keto-3-deoxygluconate | 13.9 | 12.8 | 1.09 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/E138V, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/G141S/T105I | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 11.2 | 0.46 | 24 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/G141S/T105I, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/G141S/T105I | Escherichia coli | D-2-keto-3-deoxygluconate | 5.2 | 12.1 | 0.43 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/G141S/T105I, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/T161A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 7.8 | 0.006 | 1.39 × 10³ | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/T161A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant T84A/I92F/V118A/T161A | Escherichia coli | D-2-keto-3-deoxygluconate | 0.05 | 2.4 | 0.021 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant T84A/I92F/V118A/T161A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant V118A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 108.3 | 0.21 | 516 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant V118A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase)variant V118A | Escherichia coli | D-2-keto-3-deoxygluconate | 6.1 | 48.7 | 0.13 | pH 7.5, 25 °C | PMID 11094340 | Open record: KDPG aldolase (D-2-keto-3-deoxy-6-phosphogluconate aldolase) variant V118A, D-2-keto-3-deoxygluconate |