Values CatLog read from this paper
Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates
PMID 22316217doi:10.1021/bi201899bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (42 values) from this paper: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 4.1.1.112, EC 4.1.2.14, EC 4.1.3.42
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 1 × 10⁻⁵ | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 19 | 5.7 | 3.3 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 40 | 1.7 | 24 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | 2-keto-4-hydroxyoctonoate | 1.5 | 25 | 0.06 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, 2-keto-4-hydroxyoctonoate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 175 | 43 | 4.1 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-3-deoxy-6-phosphogalactonate | 0.23 | 0.3 | 0.77 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 27 | 0.05 | 550 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-4-hydroxyoctonoate | 1.7 | 15 | 0.113 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-4-hydroxyoctonoate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 1.7 × 10⁻⁵ | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 212 | 3.5 | 61 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 31 | 0.69 | 45 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 3 × 10⁻⁵ | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 240 | 1.6 | 150 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-3-deoxy-6-phosphogalactonate | 0.006 | 1 | 0.0065 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 41 | 0.24 | 170 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-4-hydroxyoctonoate | 3.1 | 4.3 | 0.72 | pH 7.5, 25 °C | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-4-hydroxyoctonoate |