EC 4.1.1.112: oxaloacetate decarboxylase
LyasesOpen in Browse
CatLog read 58 records for EC 4.1.1.112 from 4 papers, for 3 organisms. Names in these papers: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), MhpE (putative; gene product of Rv3469c).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 35 records for EC 4.1.1.112 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.1.112 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Investigation into the Mode of Phosphate Activation in the 4-Hydroxy-4-Methyl-2-Oxoglutarate/4-Carboxy-4-Hydroxy-2-Oxoadipate Aldolase from Pseudomonas putida F1 | Pseudomonas putida | 22 |
| Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates | Escherichia coli | 19 |
| Improving upon nature: active site remodeling produces highly efficient aldolase activity toward hydrophobic electrophilic substrates | Escherichia coli | 16 |
| Characterization of an aldolase-dehydrogenase complex from the cholesterol degradation pathway of Mycobacterium tuberculosis | Mycobacterium tuberculosis | 1 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-carboxy-4-hydroxy-2-oxoadipatewithout Pi | 13.4 | 0.015 | 893 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-carboxy-4-hydroxy-2-oxoadipate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-carboxy-4-hydroxy-2-oxoadipate+ 2 mM Pi (sodium phosphate) | 145 | 0.104 | 1.4 × 10³ | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-carboxy-4-hydroxy-2-oxoadipate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 15.6 | 0.188 | 83 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 134 | 1.36 | 98.5 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | oxaloacetatewithout Pi | 1.86 | 0.298 | 6.24 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), oxaloacetate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | oxaloacetate+ 2 mM Pi (sodium phosphate) | 3.16 | 0.741 | 4.26 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), oxaloacetate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant G144V | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.215 | 1.39 | 0.155 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant G144V, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant G144V | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.394 | 6.83 | 0.0577 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant G144V, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant H75A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 22.3 | 0.389 | 57.3 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant H75A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant H75A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 53.5 | 1.21 | 44.5 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant H75A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant K147A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.014 | 2.01 | 0.00697 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant K147A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant K147A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.0224 | 3.72 | 0.00645 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant K147A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant N71A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.014 | 0.796 | 0.0176 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant N71A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant N71A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.0184 | 1.11 | 0.0165 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant N71A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R123K | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.086 | 3.21 | 0.0267 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R123K, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R123K | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.0701 | 3.97 | 0.0176 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R123K, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R195A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 6.72 | 1.37 | 4.91 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R195A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R195A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 11.5 | 2.89 | 3.98 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R195A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R40A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 6.7 | 0.304 | 22 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R40A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R40A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 36.6 | 3.26 | 11.2 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R40A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant T145A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.889 | 1.08 | 0.823 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant T145A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant T145A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 10.7 | 1.78 | 6.01 | pH 8, 25 °C | PMID 27741265 | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant T145A, 4-hydroxy-4-methyl-2-oxoglutarate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 1 × 10⁻⁵ | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 11 | 32 | 0.33 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 2 | 150 | 0.014 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxy-6-phosphogalactonate | 0.0063 | 0.1 | 0.061 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 83 | 0.1 | 830 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) | Escherichia coli | D-2-keto-3-deoxygluconate | 0.019 | 1.9 | 0.0097 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase), D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 27 | 3 | 9 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 2.6 | 56 | 0.047 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 97 | 0.16 | 610 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184A | Escherichia coli | D-2-keto-3-deoxygluconate | 0.04 | 2.3 | 0.017 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184A, D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 6 | 3 | 2 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 1.8 | 51 | 0.035 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | D-2-keto-3-deoxy-6-phosphogalactonate | 2.9 × 10⁻⁴ | 1.2 | 2.4 × 10⁻⁴ | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, D-2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 3.2 | 1.2 | 2.7 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184D | Escherichia coli | D-2-keto-3-deoxygluconate | 0.11 | 1.8 | 0.062 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184D, D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 19 | 5.7 | 3.3 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 40 | 1.7 | 24 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184F | Escherichia coli | 2-keto-4-hydroxyoctonoate | 1.5 | 25 | 0.06 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184F, 2-keto-4-hydroxyoctonoate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 40 | 3 | 13.3 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | (RS)-2-keto-4-hydroxyoctonate | 2.9 | 26 | 0.11 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, (RS)-2-keto-4-hydroxyoctonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | D-2-keto-3-deoxy-6-phosphogalactonate | 0.0026 | 0.95 | 0.0027 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, D-2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | D-2-keto-3-deoxy-6-phosphogluconate | 74 | 0.35 | 210 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, D-2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant S184L | Escherichia coli | D-2-keto-3-deoxygluconate | 0.08 | 1.5 | 0.053 | pH 7.5, 25 °C | PMID 17962400 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant S184L, D-2-keto-3-deoxygluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 175 | 43 | 4.1 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-3-deoxy-6-phosphogalactonate | 0.23 | 0.3 | 0.77 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 27 | 0.05 | 550 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S | Escherichia coli | 2-keto-4-hydroxyoctonoate | 1.7 | 15 | 0.113 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S, 2-keto-4-hydroxyoctonoate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 1.7 × 10⁻⁵ | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 212 | 3.5 | 61 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184F | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 31 | 0.69 | 45 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184F, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | — | — | 3 × 10⁻⁵ | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, (4R)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate | 240 | 1.6 | 150 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butyrate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-3-deoxy-6-phosphogalactonate | 0.006 | 1 | 0.0065 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-3-deoxy-6-phosphogalactonate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-3-deoxy-6-phosphogluconate | 41 | 0.24 | 170 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-3-deoxy-6-phosphogluconate |
| KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase)variant T161S/S184L | Escherichia coli | 2-keto-4-hydroxyoctonoate | 3.1 | 4.3 | 0.72 | pH 7.5, 25 °C | PMID 22316217 | Open record: KDPG aldolase (2-keto-3-deoxy-6-phosphogluconate aldolase) variant T161S/S184L, 2-keto-4-hydroxyoctonoate |
| MhpE (putative; gene product of Rv3469c) | Mycobacterium tuberculosis | oxaloacetate | 0.49 | 3.07 | 0.16 | pH 8, 25 °C | PMID 23614353 | Open record: MhpE (putative; gene product of Rv3469c), oxaloacetate |