Values CatLog read from this paper
Investigation into the Mode of Phosphate Activation in the 4-Hydroxy-4-Methyl-2-Oxoglutarate/4-Carboxy-4-Hydroxy-2-Oxoadipate Aldolase from Pseudomonas putida F1
PMID 27741265doi:10.1371/journal.pone.0164556Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (66 values) from this paper: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-carboxy-4-hydroxy-2-oxoadipatewithout Pi | 13.4 | 0.015 | 893 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-carboxy-4-hydroxy-2-oxoadipate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-carboxy-4-hydroxy-2-oxoadipate+ 2 mM Pi (sodium phosphate) | 145 | 0.104 | 1.4 × 10³ | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-carboxy-4-hydroxy-2-oxoadipate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 15.6 | 0.188 | 83 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 134 | 1.36 | 98.5 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | oxaloacetatewithout Pi | 1.86 | 0.298 | 6.24 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), oxaloacetate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | oxaloacetate+ 2 mM Pi (sodium phosphate) | 3.16 | 0.741 | 4.26 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), oxaloacetate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant G144V | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.215 | 1.39 | 0.155 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant G144V, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant G144V | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.394 | 6.83 | 0.0577 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant G144V, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant H75A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 22.3 | 0.389 | 57.3 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant H75A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant H75A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 53.5 | 1.21 | 44.5 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant H75A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant K147A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.014 | 2.01 | 0.00697 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant K147A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant K147A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.0224 | 3.72 | 0.00645 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant K147A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant N71A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.014 | 0.796 | 0.0176 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant N71A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant N71A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.0184 | 1.11 | 0.0165 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant N71A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R123K | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.086 | 3.21 | 0.0267 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R123K, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R123K | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 0.0701 | 3.97 | 0.0176 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R123K, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R195A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 6.72 | 1.37 | 4.91 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R195A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R195A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 11.5 | 2.89 | 3.98 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R195A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R40A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 6.7 | 0.304 | 22 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R40A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R40A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 36.6 | 3.26 | 11.2 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R40A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant T145A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutaratewithout Pi | 0.889 | 1.08 | 0.823 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant T145A, 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant T145A | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate+ 2 mM Pi (sodium phosphate) | 10.7 | 1.78 | 6.01 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant T145A, 4-hydroxy-4-methyl-2-oxoglutarate |