Values CatLog read from this paper
Characterization of heterodimeric alkaline phosphatases from Escherichia coli: an investigation of intragenic complementation
PMID 11099386doi:10.1006/jmbi.2000.4230Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 65 records (145 values) from this paper: alkaline phosphatase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 50 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; Table 2 | 44.4 | 0.0094 | 4.7 × 10³ | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphateTable 2 | 80.5 | 0.0211 | 3.8 × 10³ | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; Table 4 | 44 | 0.009 | 4.9 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphateTable 4 | 81 | 0.021 | 3.9 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 38 | 0.009 | 4.2 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 4 | 71 | 0.021 | 3.4 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 44 | 0.01 | 4.4 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatase | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 6 | 80 | 0.022 | 3.6 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D369N | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 0.39 | 0.0176 | 22 | pH 8, 25 °C | Open record: alkaline phosphatase variant D369N, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D369N | Escherichia coli | 4-nitrophenyl phosphate | 2.4 | 0.0597 | 40 | pH 8, 25 °C | Open record: alkaline phosphatase variant D369N, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D369N~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 0.12 | 0.0199 | 6.0 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D369N~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate | 0.71 | 0.063 | 11 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D369N~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 0.35 | 0.0183 | 19 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D369N~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate | 2.3 | 0.062 | 37 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D434E | Escherichia coli | 4-nitrophenyl phosphate | 65.6 | 0.0691 | 950 | pH 8, 25 °C | Open record: alkaline phosphatase variant D434E, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant E322K | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 0.016 | 0.0221 | 0.72 | pH 8, 25 °C | Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant E322K | Escherichia coli | 4-nitrophenyl phosphate | 0.027 | 0.011 | 2.5 | pH 8, 25 °C | Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant E322K | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; 10 mM Mg2+ added | 0.019 | 0.025 | 0.76 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant E322K | Escherichia coli | 4-nitrophenyl phosphate10 mM Mg2+ added | 0.05 | 0.0483 | 1.0 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant E341K | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 0.042 | 0.0095 | 4.4 | pH 8, 25 °C | Open record: alkaline phosphatase variant E341K, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant E341K | Escherichia coli | 4-nitrophenyl phosphate | 0.1 | 0.0374 | 2.7 | pH 8, 25 °C | Open record: alkaline phosphatase variant E341K, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; Table 2 | 0.067 | 0.0073 | 9.2 | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphateTable 2 | 0.059 | 0.1983 | 0.3 | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; 0.1 mM Zn2+ added; Table 3 | 0.071 | 0.0095 | 7.5 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphate0.1 mM Zn2+ added; Table 3 | 0.068 | 0.0272 | 2.5 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 0.005 | 0.0083 | 0.60 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5 | 0.023 | 0.217 | 0.11 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 0.072 | 0.0075 | 9.6 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5 | 0.061 | 0.204 | 0.30 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 2.1 | 0.0066 | 320 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit) | Escherichia coli | 4-nitrophenyl phosphate | 4.3 | 0.0219 | 200 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; vivo (double phoA plasmid in SM547) | 2.1 | 0.0085 | 250 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit) | Escherichia coli | 4-nitrophenyl phosphatevivo (double phoA plasmid in SM547) | 4.4 | 0.0214 | 210 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; Table 2 | 3.8 | 0.0035 | 1.1 × 10³ | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphateTable 2 | 5.8 | 0.0097 | 600 | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; 0.1 mM Zn2+ added; Table 3 | 3.8 | 0.0058 | 660 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphate0.1 mM Zn2+ added; Table 3 | 5.9 | 0.0113 | 520 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 2.4 | 0.0084 | 290 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5 | 5.4 | 0.0206 | 260 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 2.6 | 0.0088 | 300 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5 | 5.7 | 0.0208 | 270 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 1.3 | 0.0111 | 120 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit) | Escherichia coli | 4-nitrophenyl phosphate | 3.3 | 0.0241 | 140 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; vivo (double phoA plasmid in SM547) | 1.4 | 0.0098 | 140 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit) | Escherichia coli | 4-nitrophenyl phosphatevivo (double phoA plasmid in SM547) | 3.4 | 0.0227 | 150 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 2.3 | 0.0086 | 270 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate | 5.3 | 0.0191 | 280 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 2.6 | 0.0087 | 300 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S105L~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate | 5.7 | 0.0206 | 280 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant T155M | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer | 0.052 | 0.005 | 10 | pH 8, 25 °C | Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant T155M | Escherichia coli | 4-nitrophenyl phosphate | 0.32 | 0.057 | 5.6 | pH 8, 25 °C | Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant T155M | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; 0.1 mM Zn2+ added | 0.047 | 0.0067 | 7.0 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant T155M | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; 10 mM Mg2+ added | 0.051 | 0.0059 | 8.6 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant T155M | Escherichia coli | 4-nitrophenyl phosphate0.1 mM Zn2+ added | 0.29 | 0.0673 | 4.3 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant T155M | Escherichia coli | 4-nitrophenyl phosphate10 mM Mg2+ added | 0.34 | 0.0617 | 5.5 calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; Table 4 | 37 | 0.01 | 3.7 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit) | Escherichia coli | 4-nitrophenyl phosphateTable 4 | 69 | 0.021 | 3.3 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; Table 6 | 44 | 0.01 | 4.4 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit) | Escherichia coli | 4-nitrophenyl phosphatevivo (double phoA plasmid pEK352 in SM547); Table 6 | 80 | 0.022 | 3.6 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; Table 4 | 43 | 0.011 | 3.9 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphateTable 4 | 80 | 0.022 | 3.6 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 39 | 0.011 | 3.5 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 4 | 73 | 0.023 | 3.2 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography | 43 | 0.01 | 4.3 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate |
| alkaline phosphatasevariant WT~AT (charged tail) | Escherichia coli | 4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 6 | 79 | 0.023 | 3.4 × 10³ calc. | pH 8, 25 °C | Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate |