EC 3.1.3.1: alkaline phosphatase

HydrolasesOpen in Browse

CatLog read 253 records for EC 3.1.3.1 from 11 papers, for 5 organisms. Names in these papers: alkaline phosphatase, bovine intestine alkaline phosphatase (BIALP), alkaline phosphatase (VAP), liver alkaline phosphatase and 3 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 111 records for EC 3.1.3.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.3.1 in Browse.

Papers

Papers with records for EC 3.1.3.1
Paper	Organisms	Records
Characterization of heterodimeric alkaline phosphatases from Escherichia coli: an investigation of intragenic complementation	Escherichia coli	65
Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity	Bos taurus	47
Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site	Escherichia coli	34
Partial purification and some properties of human liver alkaline phosphatase	Homo sapiens	22
Properties of membrane-bound and solubilized forms of alkaline phosphatase from human liver	Homo sapiens	19
Partial purification of human intestinal alkaline phosphatase with affinity chromotography. Some properties and interaction of concanavalin A with alkaline phosphatase	Homo sapiens	16
Structural Characterization of Functionally Important Chloride Binding Sites in the Marine Vibrio Alkaline Phosphatase	Vibrio splendidus	14
Hofmeister effects on activity and stability of alkaline phosphatase	calf (Bos taurus) intestine	12
Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase	—	11
Cold-active alkaline phosphatase is irreversibly transformed into an inactive dimer by low urea concentrations	Vibrio splendidus	9
Effects of TiO₂ nanoparticles on the kinetics and conformation of bovine alkaline phosphatase	Bos taurus	4

Records

Records for EC 3.1.3.1 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
alkaline phosphatase	—	4-nitrophenyl phosphate	236	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatasevariant G149D	—	4-nitrophenyl phosphate	117	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant G149D, 4-nitrophenyl phosphate
alkaline phosphatasevariant G87A	—	4-nitrophenyl phosphate	100	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant G87A, 4-nitrophenyl phosphate
alkaline phosphatasevariant H135E	—	4-nitrophenyl phosphate	303	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant H135E, 4-nitrophenyl phosphate
alkaline phosphatasevariant H135E/G149D	—	4-nitrophenyl phosphate	361	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant H135E/G149D, 4-nitrophenyl phosphate
alkaline phosphatasevariant S338T	—	4-nitrophenyl phosphate	114	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant S338T, 4-nitrophenyl phosphate
alkaline phosphatasevariant S42G	—	4-nitrophenyl phosphate	133	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant S42G, 4-nitrophenyl phosphate
alkaline phosphatasevariant S42G/H135E	—	4-nitrophenyl phosphate	70	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant S42G/H135E, 4-nitrophenyl phosphate
alkaline phosphatasevariant S42G/S338T	—	4-nitrophenyl phosphate	71	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant S42G/S338T, 4-nitrophenyl phosphate
alkaline phosphatasevariant S86A	—	4-nitrophenyl phosphate	203	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant S86A, 4-nitrophenyl phosphate
alkaline phosphatasevariant S86A/G87A	—	4-nitrophenyl phosphate	30	—	—	pH 10, 15 °C	PMID 18411226	Open record: alkaline phosphatase variant S86A/G87A, 4-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateno added salt	—	0.69	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M KPF6 (pH readjusted to 9.8)	—	2.73	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M NaBF4 (pH readjusted to 9.8)	—	4.4	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M NaNO3 (pH readjusted to 9.8)	—	3.43	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M NaN3 (pH readjusted to 9.8)	—	1.37	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M NaCl (pH readjusted to 9.8)	—	4.42	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M KNO3 (pH readjusted to 9.8)	—	1.91	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M KCl (pH readjusted to 9.8)	—	2.52	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M Na2SO4 (pH readjusted to 9.8)	—	6.87	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M sodium acetate (pH readjusted to 9.8)	—	2.54	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 0.8 M sodium citrate (pH readjusted to 9.8)	—	7.32	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	calf (Bos taurus) intestine	p-nitrophenyl phosphateplus 1.0 M Na2SO4 (pH readjusted to 9.8)	—	25.1	—	pH 9.8, 37 °C	PMID 20025997	Open record: alkaline phosphatase, p-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; Table 2	44.4	0.0094	4.7 × 10³	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphateTable 2	80.5	0.0211	3.8 × 10³	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; Table 4	44	0.009	4.9 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphateTable 4	81	0.021	3.9 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	38	0.009	4.2 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 4	71	0.021	3.4 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	44	0.01	4.4 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 6	80	0.022	3.6 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase, 4-nitrophenyl phosphate
alkaline phosphatase	Escherichia coli	methyl phosphate	—	—	350	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase, methyl phosphate
alkaline phosphatasevariant D101A	Escherichia coli	4-nitrophenyl phosphate	36	0.0036	9.9 × 10³	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A	Escherichia coli	methyl phosphate	—	—	2.7	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A, methyl phosphate
alkaline phosphatasevariant D101A/D153A	Escherichia coli	4-nitrophenyl phosphate	2.2	0.0062	330	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/D153A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/D153A	Escherichia coli	methyl phosphate	—	—	0.061	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/D153A, methyl phosphate
alkaline phosphatasevariant D101A/D153A/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	2.4 × 10⁻⁴	0.14	0.002	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/D153A/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/D153A/K328A	Escherichia coli	4-nitrophenyl phosphate	1.6	0.011	120	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/D153A/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/E322Y	Escherichia coli	4-nitrophenyl phosphate	4.6 × 10⁻⁴	0.15	0.0031	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/E322Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	8.9 × 10⁻⁵	0.078	0.0011	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/R166S	Escherichia coli	4-nitrophenyl phosphate	0.42	0.0084	58	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/R166S, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/R166S/D153A	Escherichia coli	4-nitrophenyl phosphate	0.083	0.0048	20	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/R166S/D153A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/R166S/D153A/E322Y	Escherichia coli	4-nitrophenyl phosphate	3.2 × 10⁻⁴	0.35	9.4 × 10⁻⁴	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/R166S/D153A/E322Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/R166S/D153A/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	2.9 × 10⁻⁵	0.16	1.7 × 10⁻⁴	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101A/R166S/D153A/K328A	Escherichia coli	4-nitrophenyl phosphate	0.061	0.012	5.5	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101A/R166S/D153A/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101G	Escherichia coli	4-nitrophenyl phosphate	—	—	7.6 × 10³	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101G, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101G/R166S	Escherichia coli	4-nitrophenyl phosphate	—	—	27	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101G/R166S, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101G/R166S/D153A/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	—	—	1.3 × 10⁻⁴	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101G/R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101N/R166S/D153A/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	—	—	1.9 × 10⁻⁴	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101N/R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D101S	Escherichia coli	4-nitrophenyl phosphate	—	—	9.1 × 10³	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D101S, 4-nitrophenyl phosphate
alkaline phosphatasevariant D153A	Escherichia coli	4-nitrophenyl phosphate	7.6	0.0026	2.8 × 10³	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D153A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D153A	Escherichia coli	methyl phosphate	—	—	1.1	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D153A, methyl phosphate
alkaline phosphatasevariant D153A/E322Y	Escherichia coli	4-nitrophenyl phosphate	0.0014	5.2 × 10⁻⁴	2.3	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D153A/E322Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant D153A/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	7.3 × 10⁻⁴	0.0021	0.32	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D153A/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D153A/K328A	Escherichia coli	4-nitrophenyl phosphate	1.1	0.0024	440	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant D153A/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant D369N	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	0.39	0.0176	22	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant D369N, 4-nitrophenyl phosphate
alkaline phosphatasevariant D369N	Escherichia coli	4-nitrophenyl phosphate	2.4	0.0597	40	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant D369N, 4-nitrophenyl phosphate
alkaline phosphatasevariant D369N~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	0.12	0.0199	6.0 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant D369N~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate	0.71	0.063	11 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant D369N~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	0.35	0.0183	19 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant D369N~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate	2.3	0.062	37 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant D369N~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant D434E	Escherichia coli	4-nitrophenyl phosphate	65.6	0.0691	950	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant D434E, 4-nitrophenyl phosphate
alkaline phosphatasevariant E322K	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	0.016	0.0221	0.72	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate
alkaline phosphatasevariant E322K	Escherichia coli	4-nitrophenyl phosphate	0.027	0.011	2.5	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate
alkaline phosphatasevariant E322K	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; 10 mM Mg2+ added	0.019	0.025	0.76 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate
alkaline phosphatasevariant E322K	Escherichia coli	4-nitrophenyl phosphate10 mM Mg2+ added	0.05	0.0483	1.0 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant E322K, 4-nitrophenyl phosphate
alkaline phosphatasevariant E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	0.0034	0.0022	1.5	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant E341K	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	0.042	0.0095	4.4	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant E341K, 4-nitrophenyl phosphate
alkaline phosphatasevariant E341K	Escherichia coli	4-nitrophenyl phosphate	0.1	0.0374	2.7	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant E341K, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; Table 2	0.067	0.0073	9.2	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphateTable 2	0.059	0.1983	0.3	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; 0.1 mM Zn2+ added; Table 3	0.071	0.0095	7.5 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphate0.1 mM Zn2+ added; Table 3	0.068	0.0272	2.5 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	0.005	0.0083	0.60 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5	0.023	0.217	0.11 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	0.072	0.0075	9.6 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5	0.061	0.204	0.30 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	2.1	0.0066	320 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit)	Escherichia coli	4-nitrophenyl phosphate	4.3	0.0219	200 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; vivo (double phoA plasmid in SM547)	2.1	0.0085	250 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit)	Escherichia coli	4-nitrophenyl phosphatevivo (double phoA plasmid in SM547)	4.4	0.0214	210 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant H412Y + S105L~AT heterodimer (one H412Y subunit, one charged-tail S105L subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant K328A	Escherichia coli	4-nitrophenyl phosphate	3.4	0.0054	750	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant R166S	Escherichia coli	methyl phosphate	—	—	0.056	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S, methyl phosphate
alkaline phosphatasevariant R166S/D153A	Escherichia coli	4-nitrophenyl phosphate	0.07	0.0053	13	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S/D153A, 4-nitrophenyl phosphate
alkaline phosphatasevariant R166S/D153A/E322A/K328A	Escherichia coli	4-nitrophenyl phosphate	—	—	0.0027	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S/D153A/E322A/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant R166S/D153A/E322Y	Escherichia coli	4-nitrophenyl phosphate	0.0083	0.44	0.019	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S/D153A/E322Y, 4-nitrophenyl phosphate
alkaline phosphatasevariant R166S/D153A/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	9.6 × 10⁻⁴	0.22	0.0044	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant R166S/D153A/K328A	Escherichia coli	4-nitrophenyl phosphate	0.026	0.0072	3.6	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S/D153A/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant R166S/E322Y/K328A	Escherichia coli	4-nitrophenyl phosphate	5.7 × 10⁻⁵	0.14	3.9 × 10⁻⁴	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S/E322Y/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant R166S/K328A	Escherichia coli	4-nitrophenyl phosphate	0.016	0.063	0.24	pH 8, 25 °C	PMID 25902402	Open record: alkaline phosphatase variant R166S/K328A, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; Table 2	3.8	0.0035	1.1 × 10³	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphateTable 2	5.8	0.0097	600	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; 0.1 mM Zn2+ added; Table 3	3.8	0.0058	660 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphate0.1 mM Zn2+ added; Table 3	5.9	0.0113	520 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	2.4	0.0084	290 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5	5.4	0.0206	260 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	2.6	0.0088	300 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 5	5.7	0.0208	270 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L, 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	1.3	0.0111	120 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit)	Escherichia coli	4-nitrophenyl phosphate	3.3	0.0241	140 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; vivo (double phoA plasmid in SM547)	1.4	0.0098	140 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit)	Escherichia coli	4-nitrophenyl phosphatevivo (double phoA plasmid in SM547)	3.4	0.0227	150 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L + D369N~AT heterodimer (one S105L subunit, one charged-tail D369N subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	2.3	0.0086	270 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate	5.3	0.0191	280 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	2.6	0.0087	300 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant S105L~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate	5.7	0.0206	280 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant S105L~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant T155M	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer	0.052	0.005	10	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate
alkaline phosphatasevariant T155M	Escherichia coli	4-nitrophenyl phosphate	0.32	0.057	5.6	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate
alkaline phosphatasevariant T155M	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; 0.1 mM Zn2+ added	0.047	0.0067	7.0 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate
alkaline phosphatasevariant T155M	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; 10 mM Mg2+ added	0.051	0.0059	8.6 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate
alkaline phosphatasevariant T155M	Escherichia coli	4-nitrophenyl phosphate0.1 mM Zn2+ added	0.29	0.0673	4.3 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate
alkaline phosphatasevariant T155M	Escherichia coli	4-nitrophenyl phosphate10 mM Mg2+ added	0.34	0.0617	5.5 calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant T155M, 4-nitrophenyl phosphate
alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; Table 4	37	0.01	3.7 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit)	Escherichia coli	4-nitrophenyl phosphateTable 4	69	0.021	3.3 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; Table 6	44	0.01	4.4 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit)	Escherichia coli	4-nitrophenyl phosphatevivo (double phoA plasmid pEK352 in SM547); Table 6	80	0.022	3.6 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT + WT~AT heterodimer (one wild-type subunit, one charged-tail subunit), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; Table 4	43	0.011	3.9 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphateTable 4	80	0.022	3.6 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	39	0.011	3.5 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 4	73	0.023	3.2 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphate0.1 M Mops buffer; purified by ion-exchange chromatography	43	0.01	4.3 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatasevariant WT~AT (charged tail)	Escherichia coli	4-nitrophenyl phosphatepurified by ion-exchange chromatography; Table 6	79	0.023	3.4 × 10³ calc.	pH 8, 25 °C	PMID 11099386	Open record: alkaline phosphatase variant WT~AT (charged tail), 4-nitrophenyl phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	2-naphthyl phosphatepurified to homogeneity from human liver (soluble form)	—	0.026	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), 2-naphthyl phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	2-naphthyl phosphate	—	0.059	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), 2-naphthyl phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	2-naphthyl phosphatemembrane-bound	—	0.078	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), 2-naphthyl phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	4-nitrophenyl phosphatepurified to homogeneity from human liver (soluble form)	—	0.088	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), 4-nitrophenyl phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	4-nitrophenyl phosphate	—	0.15	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), 4-nitrophenyl phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	4-nitrophenyl phosphatemembrane-bound	—	0.39	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), 4-nitrophenyl phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	diphosphatepurified to homogeneity from human liver (soluble form)	—	0.12	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), diphosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	diphosphatemembrane-bound	—	0.21	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), diphosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	glycerol 2-phosphatepurified to homogeneity from human liver (soluble form)	—	1.5	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), glycerol 2-phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	glycerol 2-phosphate	—	2.2	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), glycerol 2-phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	glycerol 2-phosphatemembrane-bound	—	3	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), glycerol 2-phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	O-phospho-DL-threoninepurified to homogeneity from human liver (soluble form)	—	0.39	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), O-phospho-DL-threonine
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	O-phospho-DL-threoninemembrane-bound	—	0.9	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), O-phospho-DL-threonine
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	O-phospho-DL-tyrosinepurified to homogeneity from human liver (soluble form)	—	0.4	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), O-phospho-DL-tyrosine
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	O-phospho-DL-tyrosinemembrane-bound	—	0.72	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), O-phospho-DL-tyrosine
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	O-phospho-L-serinepurified to homogeneity from human liver (soluble form)	—	0.38	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), O-phospho-L-serine
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	O-phospho-L-serinemembrane-bound	—	0.68	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), O-phospho-L-serine
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	pyridoxal 5'-phosphatepurified to homogeneity from human liver (soluble form)	—	0.2	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), pyridoxal 5'-phosphate
alkaline phosphatase (liver/bone/kidney isoenzyme)	Homo sapiens	pyridoxal 5'-phosphatemembrane-bound	—	0.37	—	pH 9, 30 °C	PMID 3921059	Open record: alkaline phosphatase (liver/bone/kidney isoenzyme), pyridoxal 5'-phosphate
alkaline phosphatase (VAP)	Vibrio splendidus	4-nitrophenyl phosphate	302	0.194	1.56 × 10³	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP), 4-nitrophenyl phosphate
alkaline phosphatase (VAP)	Vibrio splendidus	p-nitrophenyl phosphateno added NaCl	231	0.0076	3 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP), p-nitrophenyl phosphate
alkaline phosphatase (VAP)	Vibrio splendidus	p-nitrophenyl phosphateplus 0.5 M NaCl	356	0.0079	4.5 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP), p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant A221D	Vibrio splendidus	p-nitrophenyl phosphateno added NaCl	258	0.0128	2 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant A221D, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant A221D	Vibrio splendidus	p-nitrophenyl phosphateplus 0.5 M NaCl	304	0.0128	2 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant A221D, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant F355W	Vibrio splendidus	4-nitrophenyl phosphate	255	0.218	1.17 × 10³	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant F355W, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant K422E	Vibrio splendidus	p-nitrophenyl phosphateno added NaCl	49	0.007	7 × 10³	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant K422E, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant K422E	Vibrio splendidus	p-nitrophenyl phosphateplus 0.5 M NaCl	88	0.0073	1.2 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant K422E, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant K422L	Vibrio splendidus	p-nitrophenyl phosphateno added NaCl	165	0.0066	2.5 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant K422L, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant K422L	Vibrio splendidus	p-nitrophenyl phosphateplus 0.5 M NaCl	223	0.0073	3.1 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant K422L, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant K422L/Y423F	Vibrio splendidus	p-nitrophenyl phosphateno added NaCl	100	0.0088	1.1 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant K422L/Y423F, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant K422L/Y423F	Vibrio splendidus	p-nitrophenyl phosphateplus 0.5 M NaCl	140	0.011	1.3 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant K422L/Y423F, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant W155F	Vibrio splendidus	4-nitrophenyl phosphate	125	0.218	573	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant W155F, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant W274F	Vibrio splendidus	4-nitrophenyl phosphate	300	0.462	650	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant W274F, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant W301F	Vibrio splendidus	4-nitrophenyl phosphate	137	0.239	573	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant W301F, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant W460F	Vibrio splendidus	4-nitrophenyl phosphate	65	0.354	184	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant W460F, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant W460Y	Vibrio splendidus	4-nitrophenyl phosphate	275	0.252	869	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant W460Y, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant W475F	Vibrio splendidus	4-nitrophenyl phosphate	44	0.194	230	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant W475F, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant W512F	Vibrio splendidus	4-nitrophenyl phosphate	313	0.212	1.48 × 10³	pH 9.8, 10 °C	PMID 27043172	Open record: alkaline phosphatase (VAP) variant W512F, 4-nitrophenyl phosphate
alkaline phosphatase (VAP)variant Y222P	Vibrio splendidus	p-nitrophenyl phosphateno added NaCl	194	0.0049	4 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant Y222P, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant Y222P	Vibrio splendidus	p-nitrophenyl phosphateplus 0.5 M NaCl	328	0.0051	6.4 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant Y222P, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant Y423F	Vibrio splendidus	p-nitrophenyl phosphateno added NaCl	138	0.0076	1.8 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant Y423F, p-nitrophenyl phosphate
alkaline phosphatase (VAP)variant Y423F	Vibrio splendidus	p-nitrophenyl phosphateplus 0.5 M NaCl	224	0.0054	4.1 × 10⁴	pH 8, 10 °C	PMID 36194497	Open record: alkaline phosphatase (VAP) variant Y423F, p-nitrophenyl phosphate
bovine intestinal alkaline phosphatase (BALP)	Bos taurus	4-nitrophenyl phosphateno TiO2 nanoparticles	0.0437	0.61	0.072 calc.	pH 9.5	PMID 42502275	Open record: bovine intestinal alkaline phosphatase (BALP), 4-nitrophenyl phosphate
bovine intestinal alkaline phosphatase (BALP)	Bos taurus	4-nitrophenyl phosphatewith 1 μM TiO2 nanoparticles (rutile)	0.0358	0.51	0.070 calc.	pH 9.5	PMID 42502275	Open record: bovine intestinal alkaline phosphatase (BALP), 4-nitrophenyl phosphate
bovine intestinal alkaline phosphatase (BALP)	Bos taurus	4-nitrophenyl phosphatewith 5 μM TiO2 nanoparticles (rutile)	0.0323	0.46	0.070 calc.	pH 9.5	PMID 42502275	Open record: bovine intestinal alkaline phosphatase (BALP), 4-nitrophenyl phosphate
bovine intestinal alkaline phosphatase (BALP)	Bos taurus	4-nitrophenyl phosphatewith 9 μM TiO2 nanoparticles (rutile)	0.0243	0.35	0.070 calc.	pH 9.5	PMID 42502275	Open record: bovine intestinal alkaline phosphatase (BALP), 4-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate50 mM diethanolamine–HCl	190	0.07	2.56 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate250 mM diethanolamine–HCl	550	0.12	4.58 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate500 mM diethanolamine–HCl	830	0.21	3.95 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1000 mM diethanolamine–HCl	1.24 × 10³	0.83	1.44 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1500 mM diethanolamine–HCl	1.78 × 10³	0.8	2.25 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate2000 mM diethanolamine–HCl	2.23 × 10³	1.03	2.14 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate3000 mM diethanolamine–HCl	2.33 × 10³	1.75	1.31 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate200 mM ethylamine–HCl	450	0.35	1.29 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate400 mM ethylamine–HCl	520	0.44	1.18 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate600 mM ethylamine–HCl	510	0.43	1.19 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate800 mM ethylamine–HCl	400	0.34	1.18 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1000 mM ethylamine–HCl	400	0.43	930	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate50 mM ethanol containing 0.05 M diethanolamine–HCl	200	0.06	3.33 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate100 mM ethanol containing 0.05 M diethanolamine–HCl	140	0.08	1.82 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate250 mM ethanol containing 0.05 M diethanolamine–HCl	80	0.04	1.92 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate500 mM ethanol containing 0.05 M diethanolamine–HCl	100	0.07	1.44 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate50 mM methylamine–HCl containing 0.05 M diethanolamine	140	0.09	1.56 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate100 mM methylamine–HCl containing 0.05 M diethanolamine	190	0.17	1.12 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate250 mM methylamine–HCl containing 0.05 M diethanolamine	170	0.22	800	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate500 mM methylamine–HCl containing 0.05 M diethanolamine	420	0.24	1.74 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1000 mM methylamine–HCl containing 0.05 M diethanolamine	330	0.62	580	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate200 mM dimethylamine–HCl containing 0.05 M diethanolamine	150	0.35	410	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate400 mM dimethylamine–HCl containing 0.05 M diethanolamine	250	0.73	350	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate600 mM dimethylamine–HCl containing 0.05 M diethanolamine	250	0.42	600	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate800 mM dimethylamine–HCl containing 0.05 M diethanolamine	280	0.56	500	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1000 mM dimethylamine–HCl containing 0.05 M diethanolamine	370	0.65	560	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate50 mM ethanolamine–HCl	610	0.3	2.03 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate250 mM ethanolamine–HCl	580	0.28	2.07 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate500 mM ethanolamine–HCl	460	0.29	1.59 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1000 mM ethanolamine–HCl	300	0.3	1 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate100 mM triethanolamine–HCl	840	0.24	3.5 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate200 mM triethanolamine–HCl	1.28 × 10³	0.25	5.12 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate400 mM triethanolamine–HCl	1.33 × 10³	0.24	5.41 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate600 mM triethanolamine–HCl	1.45 × 10³	0.33	4.39 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate800 mM triethanolamine–HCl	1.54 × 10³	0.38	4.05 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1000 mM triethanolamine–HCl	1.45 × 10³	0.31	4.67 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate200 mM N-methylethanolamine–HCl	500	0.18	2.78 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate400 mM N-methylethanolamine–HCl	1.3 × 10³	0.73	1.78 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate600 mM N-methylethanolamine–HCl	1.25 × 10³	1.11	1.13 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate800 mM N-methylethanolamine–HCl	1.65 × 10³	1.26	1.31 × 10³	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate1000 mM N-methylethanolamine–HCl	2.25 × 10³	2.53	890	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate50 mM borate–NaOH	130	0.49	260	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate100 mM borate–NaOH	170	1.27	130	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate200 mM borate–NaOH	160	2.98	50	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate300 mM borate–NaOH	140	4.53	30	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate400 mM borate–NaOH	120	8.14	20	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
bovine intestine alkaline phosphatase (BIALP)	Bos taurus	p-nitrophenyl phosphate500 mM borate–NaOH	120	10.3	10	pH 9.8, 20 °C	PMID 22112405	Open record: bovine intestine alkaline phosphatase (BIALP), p-nitrophenyl phosphate
intestinal alkaline phosphatase	Homo sapiens	1-naphthyl phosphate	—	0.0049	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, 1-naphthyl phosphate
intestinal alkaline phosphatase	Homo sapiens	1-naphthyl phosphate	—	0.071	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, 1-naphthyl phosphate
intestinal alkaline phosphatase	Homo sapiens	4-nitrophenyl phosphate	—	0.0036	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, 4-nitrophenyl phosphate
intestinal alkaline phosphatase	Homo sapiens	4-nitrophenyl phosphate	—	0.092	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, 4-nitrophenyl phosphate
intestinal alkaline phosphatase	Homo sapiens	adenosine 5'-monophosphate	—	0.0651	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, adenosine 5'-monophosphate
intestinal alkaline phosphatase	Homo sapiens	adenosine 5'-monophosphate	—	0.49	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, adenosine 5'-monophosphate
intestinal alkaline phosphatase	Homo sapiens	diphosphate	—	0.0415	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, diphosphate
intestinal alkaline phosphatase	Homo sapiens	diphosphate	—	0.81	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, diphosphate
intestinal alkaline phosphatase	Homo sapiens	Glucose 1-phosphate	—	0.0447	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, Glucose 1-phosphate
intestinal alkaline phosphatase	Homo sapiens	Glucose 1-phosphate	—	1.2	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, Glucose 1-phosphate
intestinal alkaline phosphatase	Homo sapiens	Glucose 6-phosphate	—	0.029	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, Glucose 6-phosphate
intestinal alkaline phosphatase	Homo sapiens	Glucose 6-phosphate	—	0.99	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, Glucose 6-phosphate
intestinal alkaline phosphatase	Homo sapiens	O-phosphoethanolamine	—	0.0301	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, O-phosphoethanolamine
intestinal alkaline phosphatase	Homo sapiens	O-phosphoethanolamine	—	0.9	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, O-phosphoethanolamine
intestinal alkaline phosphatase	Homo sapiens	p-Nitrophenylphosphothymidine	—	0.0385	—	pH 8.5, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, p-Nitrophenylphosphothymidine
intestinal alkaline phosphatase	Homo sapiens	p-Nitrophenylphosphothymidine	—	0.65	—	pH 10, 37 °C	PMID 823966	Open record: intestinal alkaline phosphatase, p-Nitrophenylphosphothymidine
liver alkaline phosphatase	Homo sapiens	4-nitrophenyl phosphate	—	0.0011	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, 4-nitrophenyl phosphate
liver alkaline phosphatase	Homo sapiens	4-nitrophenyl phosphate	—	0.068	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, 4-nitrophenyl phosphate
liver alkaline phosphatase	Homo sapiens	adenosine 5'-monophosphate	—	0.0213	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, adenosine 5'-monophosphate
liver alkaline phosphatase	Homo sapiens	adenosine 5'-monophosphate	—	0.52	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, adenosine 5'-monophosphate
liver alkaline phosphatase	Homo sapiens	adenosine 5'-triphosphate	—	0.0169	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, adenosine 5'-triphosphate
liver alkaline phosphatase	Homo sapiens	adenosine 5'-triphosphate	—	0.59	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, adenosine 5'-triphosphate
liver alkaline phosphatase	Homo sapiens	diphosphate	—	0.0176	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, diphosphate
liver alkaline phosphatase	Homo sapiens	diphosphate	—	0.98	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, diphosphate
liver alkaline phosphatase	Homo sapiens	glucose 1-phosphate	—	0.05	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, glucose 1-phosphate
liver alkaline phosphatase	Homo sapiens	glucose 1-phosphate	—	1.7	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, glucose 1-phosphate
liver alkaline phosphatase	Homo sapiens	glucose 6-phosphate	—	0.033	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, glucose 6-phosphate
liver alkaline phosphatase	Homo sapiens	glucose 6-phosphate	—	1.3	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, glucose 6-phosphate
liver alkaline phosphatase	Homo sapiens	glycerophosphate (alpha/beta prefix illegible in OCR: '~-Glycerophosphate')	—	0.0334	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, glycerophosphate (alpha/beta prefix illegible in OCR: '~-Glycerophosphate')
liver alkaline phosphatase	Homo sapiens	glycerophosphate (alpha/beta prefix illegible in OCR: '~-Glycerophosphate')	—	0.77	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, glycerophosphate (alpha/beta prefix illegible in OCR: '~-Glycerophosphate')
liver alkaline phosphatase	Homo sapiens	NADP	—	0.047	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, NADP
liver alkaline phosphatase	Homo sapiens	NADP	—	1.1	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, NADP
liver alkaline phosphatase	Homo sapiens	naphthylphosphate (alpha/beta prefix illegible in OCR: '~-Naphthyiphosphate')	—	0.0017	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, naphthylphosphate (alpha/beta prefix illegible in OCR: '~-Naphthyiphosphate')
liver alkaline phosphatase	Homo sapiens	naphthylphosphate (alpha/beta prefix illegible in OCR: '~-Naphthyiphosphate')	—	0.049	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, naphthylphosphate (alpha/beta prefix illegible in OCR: '~-Naphthyiphosphate')
liver alkaline phosphatase	Homo sapiens	O-phosphoethanolamine	—	0.0225	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, O-phosphoethanolamine
liver alkaline phosphatase	Homo sapiens	O-phosphoethanolamine	—	0.85	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, O-phosphoethanolamine
liver alkaline phosphatase	Homo sapiens	p-nitrophenylphosphothymidine	—	0.0209	—	pH 8.5, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, p-nitrophenylphosphothymidine
liver alkaline phosphatase	Homo sapiens	p-nitrophenylphosphothymidine	—	0.58	—	pH 10, 37 °C	PMID 945751	Open record: liver alkaline phosphatase, p-nitrophenylphosphothymidine