Values CatLog read from this paper
Structural Characterization of Functionally Important Chloride Binding Sites in the Marine Vibrio Alkaline Phosphatase
PMID 36194497doi:10.1021/acs.biochem.2c00438Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (42 values) from this paper: alkaline phosphatase (VAP) from Vibrio splendidus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alkaline phosphatase (VAP) | Vibrio splendidus | p-nitrophenyl phosphateno added NaCl | 231 | 0.0076 | 3 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP), p-nitrophenyl phosphate |
| alkaline phosphatase (VAP) | Vibrio splendidus | p-nitrophenyl phosphateplus 0.5 M NaCl | 356 | 0.0079 | 4.5 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP), p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant A221D | Vibrio splendidus | p-nitrophenyl phosphateno added NaCl | 258 | 0.0128 | 2 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant A221D, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant A221D | Vibrio splendidus | p-nitrophenyl phosphateplus 0.5 M NaCl | 304 | 0.0128 | 2 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant A221D, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant K422E | Vibrio splendidus | p-nitrophenyl phosphateno added NaCl | 49 | 0.007 | 7 × 10³ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant K422E, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant K422E | Vibrio splendidus | p-nitrophenyl phosphateplus 0.5 M NaCl | 88 | 0.0073 | 1.2 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant K422E, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant K422L | Vibrio splendidus | p-nitrophenyl phosphateno added NaCl | 165 | 0.0066 | 2.5 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant K422L, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant K422L | Vibrio splendidus | p-nitrophenyl phosphateplus 0.5 M NaCl | 223 | 0.0073 | 3.1 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant K422L, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant K422L/Y423F | Vibrio splendidus | p-nitrophenyl phosphateno added NaCl | 100 | 0.0088 | 1.1 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant K422L/Y423F, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant K422L/Y423F | Vibrio splendidus | p-nitrophenyl phosphateplus 0.5 M NaCl | 140 | 0.011 | 1.3 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant K422L/Y423F, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant Y222P | Vibrio splendidus | p-nitrophenyl phosphateno added NaCl | 194 | 0.0049 | 4 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant Y222P, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant Y222P | Vibrio splendidus | p-nitrophenyl phosphateplus 0.5 M NaCl | 328 | 0.0051 | 6.4 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant Y222P, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant Y423F | Vibrio splendidus | p-nitrophenyl phosphateno added NaCl | 138 | 0.0076 | 1.8 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant Y423F, p-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant Y423F | Vibrio splendidus | p-nitrophenyl phosphateplus 0.5 M NaCl | 224 | 0.0054 | 4.1 × 10⁴ | pH 8, 10 °C | Open record: alkaline phosphatase (VAP) variant Y423F, p-nitrophenyl phosphate |