Values CatLog read from this paper
Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase
PMID 18411226doi:10.1093/protein/gzn009Open in Browse
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CatLog read 11 records (11 values) from this paper: alkaline phosphatase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alkaline phosphatase | — | 4-nitrophenyl phosphate | 236 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant G149D | — | 4-nitrophenyl phosphate | 117 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant G149D, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant G87A | — | 4-nitrophenyl phosphate | 100 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant G87A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H135E | — | 4-nitrophenyl phosphate | 303 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant H135E, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant H135E/G149D | — | 4-nitrophenyl phosphate | 361 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant H135E/G149D, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S338T | — | 4-nitrophenyl phosphate | 114 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant S338T, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S42G | — | 4-nitrophenyl phosphate | 133 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant S42G, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S42G/H135E | — | 4-nitrophenyl phosphate | 70 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant S42G/H135E, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S42G/S338T | — | 4-nitrophenyl phosphate | 71 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant S42G/S338T, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S86A | — | 4-nitrophenyl phosphate | 203 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant S86A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant S86A/G87A | — | 4-nitrophenyl phosphate | 30 | — | — | pH 10, 15 °C | Open record: alkaline phosphatase variant S86A/G87A, 4-nitrophenyl phosphate |