Values CatLog read from this paper
Cold-active alkaline phosphatase is irreversibly transformed into an inactive dimer by low urea concentrations
PMID 27043172doi:10.1016/j.bbapap.2016.03.016Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: alkaline phosphatase (VAP) from Vibrio splendidus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alkaline phosphatase (VAP) | Vibrio splendidus | 4-nitrophenyl phosphate | 302 | 0.194 | 1.56 × 10³ | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP), 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant F355W | Vibrio splendidus | 4-nitrophenyl phosphate | 255 | 0.218 | 1.17 × 10³ | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant F355W, 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant W155F | Vibrio splendidus | 4-nitrophenyl phosphate | 125 | 0.218 | 573 | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant W155F, 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant W274F | Vibrio splendidus | 4-nitrophenyl phosphate | 300 | 0.462 | 650 | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant W274F, 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant W301F | Vibrio splendidus | 4-nitrophenyl phosphate | 137 | 0.239 | 573 | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant W301F, 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant W460F | Vibrio splendidus | 4-nitrophenyl phosphate | 65 | 0.354 | 184 | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant W460F, 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant W460Y | Vibrio splendidus | 4-nitrophenyl phosphate | 275 | 0.252 | 869 | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant W460Y, 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant W475F | Vibrio splendidus | 4-nitrophenyl phosphate | 44 | 0.194 | 230 | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant W475F, 4-nitrophenyl phosphate |
| alkaline phosphatase (VAP)variant W512F | Vibrio splendidus | 4-nitrophenyl phosphate | 313 | 0.212 | 1.48 × 10³ | pH 9.8, 10 °C | Open record: alkaline phosphatase (VAP) variant W512F, 4-nitrophenyl phosphate |