Values CatLog read from this paper
Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site
PMID 25902402doi:10.7554/elife.06181Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 34 records (80 values) from this paper: alkaline phosphatase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alkaline phosphatase | Escherichia coli | methyl phosphate | — | — | 350 | pH 8, 25 °C | Open record: alkaline phosphatase, methyl phosphate |
| alkaline phosphatasevariant D101A | Escherichia coli | 4-nitrophenyl phosphate | 36 | 0.0036 | 9.9 × 10³ | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A | Escherichia coli | methyl phosphate | — | — | 2.7 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A, methyl phosphate |
| alkaline phosphatasevariant D101A/D153A | Escherichia coli | 4-nitrophenyl phosphate | 2.2 | 0.0062 | 330 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/D153A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/D153A | Escherichia coli | methyl phosphate | — | — | 0.061 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/D153A, methyl phosphate |
| alkaline phosphatasevariant D101A/D153A/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | 2.4 × 10⁻⁴ | 0.14 | 0.002 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/D153A/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/D153A/K328A | Escherichia coli | 4-nitrophenyl phosphate | 1.6 | 0.011 | 120 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/D153A/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/E322Y | Escherichia coli | 4-nitrophenyl phosphate | 4.6 × 10⁻⁴ | 0.15 | 0.0031 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/E322Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | 8.9 × 10⁻⁵ | 0.078 | 0.0011 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/R166S | Escherichia coli | 4-nitrophenyl phosphate | 0.42 | 0.0084 | 58 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/R166S, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/R166S/D153A | Escherichia coli | 4-nitrophenyl phosphate | 0.083 | 0.0048 | 20 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/R166S/D153A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/R166S/D153A/E322Y | Escherichia coli | 4-nitrophenyl phosphate | 3.2 × 10⁻⁴ | 0.35 | 9.4 × 10⁻⁴ | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/R166S/D153A/E322Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/R166S/D153A/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | 2.9 × 10⁻⁵ | 0.16 | 1.7 × 10⁻⁴ | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101A/R166S/D153A/K328A | Escherichia coli | 4-nitrophenyl phosphate | 0.061 | 0.012 | 5.5 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101A/R166S/D153A/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101G | Escherichia coli | 4-nitrophenyl phosphate | — | — | 7.6 × 10³ | pH 8, 25 °C | Open record: alkaline phosphatase variant D101G, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101G/R166S | Escherichia coli | 4-nitrophenyl phosphate | — | — | 27 | pH 8, 25 °C | Open record: alkaline phosphatase variant D101G/R166S, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101G/R166S/D153A/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | — | — | 1.3 × 10⁻⁴ | pH 8, 25 °C | Open record: alkaline phosphatase variant D101G/R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101N/R166S/D153A/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | — | — | 1.9 × 10⁻⁴ | pH 8, 25 °C | Open record: alkaline phosphatase variant D101N/R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D101S | Escherichia coli | 4-nitrophenyl phosphate | — | — | 9.1 × 10³ | pH 8, 25 °C | Open record: alkaline phosphatase variant D101S, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D153A | Escherichia coli | 4-nitrophenyl phosphate | 7.6 | 0.0026 | 2.8 × 10³ | pH 8, 25 °C | Open record: alkaline phosphatase variant D153A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D153A | Escherichia coli | methyl phosphate | — | — | 1.1 | pH 8, 25 °C | Open record: alkaline phosphatase variant D153A, methyl phosphate |
| alkaline phosphatasevariant D153A/E322Y | Escherichia coli | 4-nitrophenyl phosphate | 0.0014 | 5.2 × 10⁻⁴ | 2.3 | pH 8, 25 °C | Open record: alkaline phosphatase variant D153A/E322Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D153A/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | 7.3 × 10⁻⁴ | 0.0021 | 0.32 | pH 8, 25 °C | Open record: alkaline phosphatase variant D153A/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant D153A/K328A | Escherichia coli | 4-nitrophenyl phosphate | 1.1 | 0.0024 | 440 | pH 8, 25 °C | Open record: alkaline phosphatase variant D153A/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | 0.0034 | 0.0022 | 1.5 | pH 8, 25 °C | Open record: alkaline phosphatase variant E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant K328A | Escherichia coli | 4-nitrophenyl phosphate | 3.4 | 0.0054 | 750 | pH 8, 25 °C | Open record: alkaline phosphatase variant K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant R166S | Escherichia coli | methyl phosphate | — | — | 0.056 | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S, methyl phosphate |
| alkaline phosphatasevariant R166S/D153A | Escherichia coli | 4-nitrophenyl phosphate | 0.07 | 0.0053 | 13 | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S/D153A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant R166S/D153A/E322A/K328A | Escherichia coli | 4-nitrophenyl phosphate | — | — | 0.0027 | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S/D153A/E322A/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant R166S/D153A/E322Y | Escherichia coli | 4-nitrophenyl phosphate | 0.0083 | 0.44 | 0.019 | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S/D153A/E322Y, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant R166S/D153A/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | 9.6 × 10⁻⁴ | 0.22 | 0.0044 | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S/D153A/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant R166S/D153A/K328A | Escherichia coli | 4-nitrophenyl phosphate | 0.026 | 0.0072 | 3.6 | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S/D153A/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant R166S/E322Y/K328A | Escherichia coli | 4-nitrophenyl phosphate | 5.7 × 10⁻⁵ | 0.14 | 3.9 × 10⁻⁴ | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S/E322Y/K328A, 4-nitrophenyl phosphate |
| alkaline phosphatasevariant R166S/K328A | Escherichia coli | 4-nitrophenyl phosphate | 0.016 | 0.063 | 0.24 | pH 8, 25 °C | Open record: alkaline phosphatase variant R166S/K328A, 4-nitrophenyl phosphate |