Values CatLog read from this paper
Kinetic alteration of a human dihydrodiol/3alpha-hydroxysteroid dehydrogenase isoenzyme, AKR1C4, by replacement of histidine-216 with tyrosine or phenylalanine
PMID 11104674doi:10.1042/bj3520685Open in Browse
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CatLog read 77 records (140 values) from this paper: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 51 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | (R)-1,2,3,4-tetrahydronaphth-1-ol | 0.18 | 0.16 | 1.1 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), (R)-1,2,3,4-tetrahydronaphth-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | (S)-1,2,3,4-tetrahydronaphth-1-ol | 0.2 | 0.11 | 1.8 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), (S)-1,2,3,4-tetrahydronaphth-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | (S)-Indan-1-ol | 0.102 | 0.146 | 0.700 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), (S)-Indan-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | 5α-Androstane-3α,17β-diol | 0.087 | 8 × 10⁻⁴ | 110 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), 5α-Androstane-3α,17β-diol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | 5β-Androstan-3α-ol-17-one | 0.048 | 9 × 10⁻⁴ | 54 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), 5β-Androstan-3α-ol-17-one |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | 5β-androstane-3α,17β-diol | 0.047 | 0.0012 | 39 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), 5β-androstane-3α,17β-diol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | Androsterone | 0.043 | 5 × 10⁻⁴ | 87 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), Androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | androsteroneno activator (control row of the activator study) | 0.043 | 5 × 10⁻⁴ | 87 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | androsteronewith 5 µM Sulphobromophthalein (non-essential activator) | 0.45 | 0.0011 | 410 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | androsteronewith 50 µM (R)-Ibuprofen (non-essential activator) | 0.17 | 7 × 10⁻⁴ | 240 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | androsteronewith 100 µM Clofibric acid (non-essential activator) | 0.107 | 8 × 10⁻⁴ | 130 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | androsteronewith 1 µM L-Thyroxine (non-essential activator) | 0.28 | 0.001 | 280 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | androsteronewith 1 µM D-Thyroxine (non-essential activator) | 0.33 | 0.0012 | 280 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | Chenodeoxycholic acid | 0.032 | 0.0011 | 29 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), Chenodeoxycholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | Cholic acid | 0.2 | 0.059 | 3.4 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), Cholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | Deoxycholic acid | 0.085 | 0.0097 | 8.8 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), Deoxycholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | Lithocholic acid | 0.032 | 0.001 | 32 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), Lithocholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NAD+ | 0.2 | 1.2 | 0.17 | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NAD+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NADP+fluorimetric NAD(P)H production; Table 1 | 0.04 | 0.0017 | 23 | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0016 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0019 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0017 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0018 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0019 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0017 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4), NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | (R)-1,2,3,4-tetrahydronaphth-1-ol | 0.38 | 0.38 | 1.0 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, (R)-1,2,3,4-tetrahydronaphth-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | (S)-1,2,3,4-tetrahydronaphth-1-ol | 0.27 | 0.24 | 1.1 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, (S)-1,2,3,4-tetrahydronaphth-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | (S)-Indan-1-ol | 0.27 | 0.28 | 0.95 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, (S)-Indan-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | 5α-Androstane-3α,17β-diol | 0.3 | 0.0031 | 97 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, 5α-Androstane-3α,17β-diol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | 5β-Androstan-3α-ol-17-one | 0.135 | 0.0014 | 96 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, 5β-Androstan-3α-ol-17-one |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | 5β-androstane-3α,17β-diol | 0.165 | 0.0021 | 79 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, 5β-androstane-3α,17β-diol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | Androsterone | 0.153 | 0.0022 | 70 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, Androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | androsteroneno activator (control row of the activator study) | 0.153 | 0.0026 | 59 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | androsteronewith 5 µM Sulphobromophthalein (non-essential activator) | 0.48 | 0.023 | 21 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | androsteronewith 50 µM (R)-Ibuprofen (non-essential activator) | 0.22 | 0.0083 | 26 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | androsteronewith 100 µM Clofibric acid (non-essential activator) | 0.25 | 0.01 | 25 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | androsteronewith 1 µM L-Thyroxine (non-essential activator) | 0.32 | 0.014 | 23 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | androsteronewith 1 µM D-Thyroxine (non-essential activator) | 0.32 | 0.013 | 24 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | Chenodeoxycholic acid | 0.105 | 0.0023 | 46 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, Chenodeoxycholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | Cholic acid | 0.03 | 0.032 | 0.94 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, Cholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | Deoxycholic acid | 0.012 | 0.0014 | 8.3 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, Deoxycholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | Lithocholic acid | 0.04 | 0.0018 | 22 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, Lithocholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NAD+ | 0.33 | 1.2 | 0.28 | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NAD+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NADP+fluorimetric NAD(P)H production; Table 1 | 0.16 | 6 × 10⁻⁴ | 270 | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 5 × 10⁻⁴ | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0014 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 6 × 10⁻⁴ | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0011 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0013 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216F | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0015 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216F, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216W | Homo sapiens | androsterone | — | 0.0072 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216W, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216W | Homo sapiens | NADP+ | — | 0.0067 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216W, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | (R)-1,2,3,4-tetrahydronaphth-1-ol | 0.77 | 0.68 | 1.1 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, (R)-1,2,3,4-tetrahydronaphth-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | (S)-1,2,3,4-tetrahydronaphth-1-ol | 0.43 | 0.29 | 1.5 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, (S)-1,2,3,4-tetrahydronaphth-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | (S)-Indan-1-ol | 0.4 | 0.42 | 0.95 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, (S)-Indan-1-ol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | 5α-Androstane-3α,17β-diol | 0.35 | 0.0026 | 130 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, 5α-Androstane-3α,17β-diol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | 5β-Androstan-3α-ol-17-one | 0.18 | 0.0018 | 100 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, 5β-Androstan-3α-ol-17-one |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | 5β-androstane-3α,17β-diol | 0.18 | 0.0022 | 83 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, 5β-androstane-3α,17β-diol |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | Androsterone | 0.28 | 0.0027 | 100 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, Androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | androsteroneno activator (control row of the activator study) | 0.3 | 0.0022 | 140 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | androsteronewith 5 µM Sulphobromophthalein (non-essential activator) | 0.83 | 0.023 | 36 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | androsteronewith 50 µM (R)-Ibuprofen (non-essential activator) | 0.38 | 0.005 | 77 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | androsteronewith 100 µM Clofibric acid (non-essential activator) | 0.43 | 0.011 | 39 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | androsteronewith 1 µM L-Thyroxine (non-essential activator) | 0.67 | 0.02 | 33 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | androsteronewith 1 µM D-Thyroxine (non-essential activator) | 0.62 | 0.013 | 47 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, androsterone |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | Chenodeoxycholic acid | 0.133 | 0.0029 | 46 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, Chenodeoxycholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | Cholic acid | 0.05 | 0.041 | 1.2 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, Cholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | Deoxycholic acid | 0.018 | 0.0013 | 14 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, Deoxycholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | Lithocholic acid | 0.043 | 0.0019 | 23 calc. | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, Lithocholic acid |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NAD+ | 0.58 | 1 | 0.58 | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NAD+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NADP+fluorimetric NAD(P)H production; Table 1 | 0.25 | 5 × 10⁻⁴ | 500 | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 5 × 10⁻⁴ | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0025 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.001 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 9 × 10⁻⁴ | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0018 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NADP+ |
| dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4)variant H216Y | Homo sapiens | NADP+androsterone dehydrogenase assay with NADP+; Table 4 | — | 0.0019 | — | pH 7.4, 25 °C | Open record: dihydrodiol dehydrogenase/3alpha-hydroxysteroid dehydrogenase isoenzyme AKR1C4 (DD4) variant H216Y, NADP+ |