Values CatLog read from this paper

Engineering the substrate specificity of Escherichia coli asparaginase. II. Selective reduction of glutaminase activity by amino acid replacements at position 248

PMID 11106175doi:10.1110/ps.9.10.2009Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 33 records (95 values) from this paper: L-asparaginase II (EcA2) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.1.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
L-asparaginase II (EcA2)	Escherichia coli	L-asparagine	24	0.015	1.6 × 10³	pH 7, 25 °C	Open record: L-asparaginase II (EcA2), L-asparagine
L-asparaginase II (EcA2)	Escherichia coli	L-aspartic acid β-hydroxamate	29	0.035	830	pH 7, 25 °C	Open record: L-asparaginase II (EcA2), L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)	Escherichia coli	L-glutamine	0.33	3.5	0.094	pH 7, 25 °C	Open record: L-asparaginase II (EcA2), L-glutamine
L-asparaginase II (EcA2)variant G11L	Escherichia coli	L-aspartic acid β-hydroxamate	—	0.13	—	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G11L, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G11V	Escherichia coli	L-aspartic acid β-hydroxamate	0.11	0.07	1.6	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G11V, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57A	Escherichia coli	L-aspartic acid β-hydroxamate	15.4	0.069	220	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G57A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57A	Escherichia coli	L-glutamine	0.01	5.7	0.018	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G57A, L-glutamine
L-asparaginase II (EcA2)variant G57L	Escherichia coli	L-aspartic acid β-hydroxamate	0.2	0.082	2.4	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G57L, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57V	Escherichia coli	L-aspartic acid β-hydroxamate	0.86	0.05	17	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G57V, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57V	Escherichia coli	L-glutamine	0.006	2.4	0.0025	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G57V, L-glutamine
L-asparaginase II (EcA2)variant G88A	Escherichia coli	L-aspartic acid β-hydroxamate	0.004	0.04	0.1	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G88A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G88I	Escherichia coli	L-aspartic acid β-hydroxamate	—	0.05	—	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant G88I, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248A	Escherichia coli	L-asparagine	7	0.095	74	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248A, L-asparagine
L-asparaginase II (EcA2)variant N248A	Escherichia coli	L-aspartic acid β-hydroxamate	27	0.19	140	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248A	Escherichia coli	L-glutamine	0.0029	16	2 × 10⁻⁴	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248A, L-glutamine
L-asparaginase II (EcA2)variant N248D	Escherichia coli	L-aspartic acid β-hydroxamate	4.6	0.056	82	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248D, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248D	Escherichia coli	L-glutamine	0.0068	3.5	0.0019	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248D, L-glutamine
L-asparaginase II (EcA2)variant N248E	Escherichia coli	L-aspartic acid β-hydroxamate	26	0.14	190	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248E, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248E	Escherichia coli	L-glutamine	0.019	70	0.0027	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248E, L-glutamine
L-asparaginase II (EcA2)variant N248G	Escherichia coli	L-aspartic acid β-hydroxamate	23	0.21	110	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248G, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248G	Escherichia coli	L-glutamine	0.0046	6	8 × 10⁻⁴	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248G, L-glutamine
L-asparaginase II (EcA2)variant N248Q	Escherichia coli	L-aspartic acid β-hydroxamate	21	0.15	140	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248Q, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248Q	Escherichia coli	L-glutamine	0.032	21	0.0015	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant N248Q, L-glutamine
L-asparaginase II (EcA2)variant Q59A	Escherichia coli	L-aspartic acid β-hydroxamate	9.7	1.9	5.1	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant Q59A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant Q59A	Escherichia coli	L-glutamine	0.001	10	1 × 10⁻⁴	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant Q59A, L-glutamine
L-asparaginase II (EcA2)variant Q59E	Escherichia coli	L-aspartic acid β-hydroxamate	2	0.037	54	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant Q59E, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant Q59E	Escherichia coli	L-glutamine	0.0024	23	0.001	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant Q59E, L-glutamine
L-asparaginase II (EcA2)variant Q59G	Escherichia coli	L-aspartic acid β-hydroxamate	14.3	1.8	7.9	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant Q59G, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant Q59G	Escherichia coli	L-glutamine	0.01	50	2 × 10⁻⁴	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant Q59G, L-glutamine
L-asparaginase II (EcA2)variant V27L	Escherichia coli	L-aspartic acid β-hydroxamate	10.3	0.011	940	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant V27L, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant V27L	Escherichia coli	L-glutamine	0.091	4.4	0.021	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant V27L, L-glutamine
L-asparaginase II (EcA2)variant V27M	Escherichia coli	L-aspartic acid β-hydroxamate	10.8	0.015	720	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant V27M, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant V27M	Escherichia coli	L-glutamine	0.032	4	0.008	pH 7, 25 °C	Open record: L-asparaginase II (EcA2) variant V27M, L-glutamine