EC 3.5.1.1: asparaginase

HydrolasesOpen in Browse

CatLog read 175 records for EC 3.5.1.1 from 15 papers, for 12 organisms. Names in these papers: L-asparaginase II (EcA2), ASPGB1 (K+-dependent L-asparaginase), asparaginase II, ASPGA1 (K+-independent L-asparaginase) and 15 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 18 records for EC 3.5.1.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.1.1 in Browse.

Papers

Papers with records for EC 3.5.1.1
Paper	Organisms	Records
Role of asparaginase variable loop at the carboxyl terminal of the alpha subunit in the determination of substrate preference in plants	Arabidopsis thaliana	49
Engineering the substrate specificity of Escherichia coli asparaginase. II. Selective reduction of glutaminase activity by amino acid replacements at position 248	Escherichia coli	33
Reactions of asparaginase II of Saccharomyces cerevisiae. A mechanistic analysis of hydrolysis and hydroxylaminolysis	Saccharomyces cerevisiae	21
Biochemical characterization of L-asparaginase isoforms from Rhizobium etli-the boosting effect of zinc	Rhizobium etli	17
Hyperthermophilic asparaginase mutants with enhanced substrate affinity and antineoplastic activity: structural insights on their mechanism of action	Pyrococcus furiosus	8
doi:10.1016/j.procbio.2018.05.002	Bacillus subtilis	8
Engineering of Helicobacter pylori L-asparaginase: characterization of two functionally distinct groups of mutants	Helicobacter pylori	7
Catalytic Role of the Substrate Defines Specificity of Therapeutic l-Asparaginase	Escherichia coli	7
Co-occurrence of both L-asparaginase subtypes in Arabidopsis: At3g16150 encodes a K+-dependent L-asparaginase	Arabidopsis thaliana	5
Expression, purification and catalytic activity of Lupinus luteus asparagine beta-amidohydrolase and its Escherichia coli homolog	Escherichia coli, Lupinus luteus	4
L-Asparaginase from Erwinia Chrysanthemi 3937: cloning, expression and characterization	Erwinia chrysanthemi	4
The differential ability of asparagine and glutamine in promoting the closed/active enzyme conformation rationalizes the Wolinella succinogenes L-asparaginase substrate specificity	Wolinella succinogenes	4
A protease-resistant Escherichia coli asparaginase with outstanding stability and enhanced anti-leukaemic activity in vitro	Escherichia coli	4
Novel glutaminase-free l-asparaginase from Leuconostoc mesenteroides BN1: biochemical properties, molecular dynamics, and anticancer activity	Leuconostoc mesenteroides	3
A human-like glutaminase-free asparaginase is highly efficacious in ASNS^low leukemia and solid cancer mouse xenograft models	Cavia porcellus	1

Records

Records for EC 3.5.1.1 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
asparaginase II	Saccharomyces cerevisiae	hydroxylamine	—	7	—	pH 6.5, 29 °C	PMID 6986375	Open record: asparaginase II, hydroxylamine
asparaginase II	Saccharomyces cerevisiae	hydroxylamine	—	6.8	—	pH 6.8	PMID 6986375	Open record: asparaginase II, hydroxylamine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	1.65	—	pH 4.5, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	1.4	—	pH 4.9, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparaginehydrolysis; glutamic-oxalacetic transaminase-coupled assay	—	0.6	—	29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparaginehydrolysis; glutamic-oxalacetic transaminase-coupled assay	—	0.38	—	29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparaginehydrolysis; glutamic-oxalacetic transaminase-coupled assay	—	0.19	—	29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparaginehydrolysis; glutamic-oxalacetic transaminase-coupled assay	—	0.13	—	29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.74	—	pH 5.7, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.3	—	pH 6.8, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.25	—	pH 7, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparaginehydroxylaminolysis; 20 mM phosphate buffer; pH 5.2-5.3	—	1.43	—	29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparaginehydroxylaminolysis; 20 mM phosphate buffer; pH 6.0-6.1	—	0.8	—	29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparaginehydroxylaminolysis; 20 mM phosphate buffer; pH 6.4-6.5	—	0.52	—	29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	1.33	—	pH 5.6, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.3	—	pH 6.9, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.16	—	pH 7.6, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.11	—	pH 8.1, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagineapparent Km	—	0.55	—	pH 6.5, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.52	—	pH 6.5, 29 °C	PMID 6986375	Open record: asparaginase II, L-asparagine
asparaginase II	Saccharomyces cerevisiae	L-asparagine	—	0.26	—	pH 6.8	PMID 6986375	Open record: asparaginase II, L-asparagine
ASPGA1 (K+-independent L-asparaginase)	Arabidopsis thaliana	beta-aspartyl-histidine	—	12.6	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase), beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)	Arabidopsis thaliana	L-asparagine	—	14.7	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase), L-asparagine
ASPGA1 (K+-independent L-asparaginase)chimera A	Arabidopsis thaliana	beta-aspartyl-histidine	—	1.01	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A, beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)chimera A	Arabidopsis thaliana	L-asparagine	—	0.13	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A, L-asparagine
ASPGA1 (K+-independent L-asparaginase)chimera A Δ169–173	Arabidopsis thaliana	beta-aspartyl-histidine	—	0.67	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A Δ169–173, beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)chimera A Δ169–173	Arabidopsis thaliana	L-asparagine	—	0.26	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A Δ169–173, L-asparagine
ASPGA1 (K+-independent L-asparaginase)chimera A Δ169–178	Arabidopsis thaliana	L-asparagine	—	0.7	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A Δ169–178, L-asparagine
ASPGA1 (K+-independent L-asparaginase)chimera A Δ181–184	Arabidopsis thaliana	beta-aspartyl-histidine	—	0.083	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A Δ181–184, beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)chimera A Δ181–184	Arabidopsis thaliana	L-asparagine	—	0.19	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A Δ181–184, L-asparagine
ASPGA1 (K+-independent L-asparaginase)chimera A Δ181–189	Arabidopsis thaliana	beta-aspartyl-histidine	—	0.26	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A Δ181–189, beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)chimera A Δ181–189	Arabidopsis thaliana	L-asparagine	—	0.94	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera A Δ181–189, L-asparagine
ASPGA1 (K+-independent L-asparaginase)chimera B	Arabidopsis thaliana	beta-aspartyl-histidine	—	0.52	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera B, beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)chimera B	Arabidopsis thaliana	L-asparagine	—	0.046	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) chimera B, L-asparagine
ASPGA1 (K+-independent L-asparaginase)variant L163F	Arabidopsis thaliana	beta-aspartyl-histidine	—	6.79	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) variant L163F, beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)variant L163F	Arabidopsis thaliana	L-asparagine	—	12.7	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) variant L163F, L-asparagine
ASPGA1 (K+-independent L-asparaginase)variant T166R	Arabidopsis thaliana	beta-aspartyl-histidine	—	3.13	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) variant T166R, beta-aspartyl-histidine
ASPGA1 (K+-independent L-asparaginase)variant T166R	Arabidopsis thaliana	L-asparagine	—	14.3	—	pH 8	PMID 22127737	Open record: ASPGA1 (K+-independent L-asparaginase) variant T166R, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)	Arabidopsis thaliana	beta-aspartyl-histidine	—	2.38	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase), beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)	Arabidopsis thaliana	L-asparagine	—	6.83	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase), L-asparagine
ASPGB1 (K+-dependent L-asparaginase)chimera C	Arabidopsis thaliana	beta-aspartyl-histidine	—	3	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) chimera C, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)chimera C	Arabidopsis thaliana	L-asparagine	—	20.9	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) chimera C, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)chimera D	Arabidopsis thaliana	beta-aspartyl-histidine	—	0.23	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) chimera D, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)chimera D	Arabidopsis thaliana	L-asparagine	—	4.04	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) chimera D, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant F162L	Arabidopsis thaliana	beta-aspartyl-histidine	—	5.56	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant F162L, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant F162L	Arabidopsis thaliana	L-asparagine	—	3.25	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant F162L, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant F162W	Arabidopsis thaliana	beta-aspartyl-histidine	—	3.9	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant F162W, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant F162W	Arabidopsis thaliana	L-asparagine	—	5.12	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant F162W, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant N184A	Arabidopsis thaliana	beta-aspartyl-histidine	—	2.9	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant N184A, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant N184A	Arabidopsis thaliana	L-asparagine	—	4.53	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant N184A, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant N184D	Arabidopsis thaliana	beta-aspartyl-histidine	—	4.15	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant N184D, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant N184D	Arabidopsis thaliana	L-asparagine	—	4.11	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant N184D, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant N184Q	Arabidopsis thaliana	beta-aspartyl-histidine	—	3.28	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant N184Q, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant N184Q	Arabidopsis thaliana	L-asparagine	—	10.5	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant N184Q, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant R165T	Arabidopsis thaliana	beta-aspartyl-histidine	—	5.62	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant R165T, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant R165T	Arabidopsis thaliana	L-asparagine	—	10.7	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant R165T, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant S189A	Arabidopsis thaliana	beta-aspartyl-histidine	—	4.91	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant S189A, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant S189A	Arabidopsis thaliana	L-asparagine	—	4.23	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant S189A, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant S189C	Arabidopsis thaliana	beta-aspartyl-histidine	—	3.56	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant S189C, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant S189C	Arabidopsis thaliana	L-asparagine	—	4.72	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant S189C, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant S189T	Arabidopsis thaliana	beta-aspartyl-histidine	—	6.65	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant S189T, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant S189T	Arabidopsis thaliana	L-asparagine	—	3.25	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant S189T, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant Δ169–173	Arabidopsis thaliana	beta-aspartyl-histidine	—	3.77	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ169–173, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant Δ169–173	Arabidopsis thaliana	L-asparagine	—	2.63	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ169–173, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant Δ169–178	Arabidopsis thaliana	beta-aspartyl-histidine	—	3.8	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ169–178, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant Δ169–178	Arabidopsis thaliana	L-asparagine	—	3.95	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ169–178, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant Δ181–184	Arabidopsis thaliana	beta-aspartyl-histidine	—	1.03	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ181–184, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant Δ181–184	Arabidopsis thaliana	L-asparagine	—	3.12	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ181–184, L-asparagine
ASPGB1 (K+-dependent L-asparaginase)variant Δ181–189	Arabidopsis thaliana	beta-aspartyl-histidine	—	2.29	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ181–189, beta-aspartyl-histidine
ASPGB1 (K+-dependent L-asparaginase)variant Δ181–189	Arabidopsis thaliana	L-asparagine	—	2.6	—	pH 8	PMID 22127737	Open record: ASPGB1 (K+-dependent L-asparaginase) variant Δ181–189, L-asparagine
EcAIII (Escherichia coli iaaA gene product; plant-type asparaginase / isoaspartyl peptidase)	Escherichia coli	beta-L-aspartyl-L-leucine	0.631	0.138	4.57	pH 7.5	PMID 15265041	Open record: EcAIII (Escherichia coli iaaA gene product; plant-type asparaginase / isoaspartyl peptidase), beta-L-aspartyl-L-leucine
EcAIII (Escherichia coli iaaA gene product; plant-type asparaginase / isoaspartyl peptidase)	Escherichia coli	L-asparagine	0.28	3.9	0.0718	pH 7.5	PMID 15265041	Open record: EcAIII (Escherichia coli iaaA gene product; plant-type asparaginase / isoaspartyl peptidase), L-asparagine
K+-dependent L-asparaginase At3g16150	Arabidopsis thaliana	L-asparagine	63.5	0.89	71.3	pH 7.2, 24 °C	PMID 16705405	Open record: K+-dependent L-asparaginase At3g16150, L-asparagine
K+-dependent L-asparaginase At3g16150	Arabidopsis thaliana	L-aspartic beta-hydroxamate	58.3	1.08	54	pH 7.3, 37 °C	PMID 16705405	Open record: K+-dependent L-asparaginase At3g16150, L-aspartic beta-hydroxamate
K+-independent L-asparaginase At5g08100	Arabidopsis thaliana	beta-aspartyl-histidine	4.58	7.69	0.6	pH 7.2, 24 °C	PMID 16705405	Open record: K+-independent L-asparaginase At5g08100, beta-aspartyl-histidine
K+-independent L-asparaginase At5g08100	Arabidopsis thaliana	L-asparagine	3.25	3.78	0.86	pH 7.2, 24 °C	PMID 16705405	Open record: K+-independent L-asparaginase At5g08100, L-asparagine
K+-independent L-asparaginase At5g08100	Arabidopsis thaliana	L-aspartic beta-hydroxamate	2.48	17.3	0.14	pH 7.3, 37 °C	PMID 16705405	Open record: K+-independent L-asparaginase At5g08100, L-aspartic beta-hydroxamate
L-asparaginase (ASN)	Bacillus subtilis	L-asparagine	48.5	4.99	9.72	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN), L-asparagine
L-asparaginase (ASN)variant D289T	Bacillus subtilis	L-asparagine	43.16	3.88	11.13	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN) variant D289T, L-asparagine
L-asparaginase (ASN)variant E260F	Bacillus subtilis	L-asparagine	32.16	3.73	8.63	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN) variant E260F, L-asparagine
L-asparaginase (ASN)variant E292S	Bacillus subtilis	L-asparagine	34.49	3.16	10.9	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN) variant E292S, L-asparagine
L-asparaginase (ASN)variant S180N	Bacillus subtilis	L-asparagine	47.94	3.91	12.26	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN) variant S180N, L-asparagine
L-asparaginase (ASN)variant S180N/D289T	Bacillus subtilis	L-asparagine	38.07	2.59	14.72	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN) variant S180N/D289T, L-asparagine
L-asparaginase (ASN)variant S180N/D289T/E260F	Bacillus subtilis	L-asparagine	25.02	2.21	11.32	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN) variant S180N/D289T/E260F, L-asparagine
L-asparaginase (ASN)variant S180N/D289T/E260F/E292S	Bacillus subtilis	L-asparagine	24.47	2.06	11.88	37 °C	doi:10.1016/j.procbio.2018.05.002	Open record: L-asparaginase (ASN) variant S180N/D289T/E260F/E292S, L-asparagine
L-asparaginase (asparaginase, EBD-200)variant EBD-200 (humanized GpA369: surface and T-cell-epitope residues mutated to the human asparaginase sequence)	Cavia porcellus	L-asparagine	61.7	0.0413	1.49 × 10³ calc.	pH 7.5, 37 °C	PMID 39709177	Open record: L-asparaginase (asparaginase, EBD-200) variant EBD-200 (humanized GpA369: surface and T-cell-epitope residues mutated to the human asparaginase sequence), L-asparagine
L-asparaginase (Er L-ASNase)	Erwinia chrysanthemi	L-asparagine	23.8	0.058	411.8	pH 8, 37 °C	PMID 16984804	Open record: L-asparaginase (Er L-ASNase), L-asparagine
L-asparaginase (Er L-ASNase)	Erwinia chrysanthemi	L-glutamine	4.3	6.7	0.6	pH 8.2, 37 °C	PMID 16984804	Open record: L-asparaginase (Er L-ASNase), L-glutamine
L-asparaginase (Er L-ASNase)	Erwinia chrysanthemi	N2-acetyl-L-asparagine	10.8	0.8	13.4	pH 8, 37 °C	PMID 16984804	Open record: L-asparaginase (Er L-ASNase), N2-acetyl-L-asparagine
L-asparaginase (Er L-ASNase)	Erwinia chrysanthemi	succinamic acid	23.9	18.8	1.3	pH 8, 37 °C	PMID 16984804	Open record: L-asparaginase (Er L-ASNase), succinamic acid
L-asparaginase (HpASNase, type II)	Helicobacter pylori	L-asparagine	19.26	—	—	pH 7.5, 37 °C	PMID 25664771	Open record: L-asparaginase (HpASNase, type II), L-asparagine
L-asparaginase (HpASNase, type II)	Helicobacter pylori	L-glutamine	22.1	—	—	pH 7.5, 37 °C	PMID 25664771	Open record: L-asparaginase (HpASNase, type II), L-glutamine
L-asparaginase (HpASNase, type II)variant M121C	Helicobacter pylori	L-asparagine	4.83	—	—	pH 7.5, 37 °C	PMID 25664771	Open record: L-asparaginase (HpASNase, type II) variant M121C, L-asparagine
L-asparaginase (HpASNase, type II)variant M121C/T169M	Helicobacter pylori	L-asparagine	13.95	—	—	pH 7.5, 37 °C	PMID 25664771	Open record: L-asparaginase (HpASNase, type II) variant M121C/T169M, L-asparagine
L-asparaginase (HpASNase, type II)variant Q63E	Helicobacter pylori	L-asparagine	6.53	—	—	pH 7.5, 37 °C	PMID 25664771	Open record: L-asparaginase (HpASNase, type II) variant Q63E, L-asparagine
L-asparaginase (HpASNase, type II)variant Q63E	Helicobacter pylori	L-glutamine	14.5	—	—	pH 7.5, 37 °C	PMID 25664771	Open record: L-asparaginase (HpASNase, type II) variant Q63E, L-glutamine
L-asparaginase (HpASNase, type II)variant T169M	Helicobacter pylori	L-asparagine	7.4	—	—	pH 7.5, 37 °C	PMID 25664771	Open record: L-asparaginase (HpASNase, type II) variant T169M, L-asparagine
L-asparaginase (LmASNase)	Leuconostoc mesenteroides	L-asparagine	26.94	—	—	pH 6, 37 °C	PMID 42770085	Open record: L-asparaginase (LmASNase), L-asparagine
L-asparaginase (LmASNase)	Leuconostoc mesenteroides	L-asparagine50 mM phosphate buffer pH 7.5; no added metal ions	18.57	—	—	pH 7.5, 37 °C	PMID 42770085	Open record: L-asparaginase (LmASNase), L-asparagine
L-asparaginase (LmASNase)	Leuconostoc mesenteroides	L-asparagine50 mM phosphate buffer pH 7.5 + 1 mM Zn2+	3.94	—	—	pH 7.5, 37 °C	PMID 42770085	Open record: L-asparaginase (LmASNase), L-asparagine
L-asparaginase (PfA)	Pyrococcus furiosus	L-asparagine	888.6	12.1	73	pH 8.2, 80 °C	PMID 22166247	Open record: L-asparaginase (PfA), L-asparagine
L-asparaginase (PfA)	Pyrococcus furiosus	L-asparagine	17.98	8.12	2.21	pH 7.4, 37 °C	PMID 22166247	Open record: L-asparaginase (PfA), L-asparagine
L-asparaginase (PfA)variant K274E	Pyrococcus furiosus	L-asparagine	199.2	2.1	96	pH 8.2, 80 °C	PMID 22166247	Open record: L-asparaginase (PfA) variant K274E, L-asparagine
L-asparaginase (PfA)variant K274E	Pyrococcus furiosus	L-asparagine	24.9	4.3	5.78	pH 7.4, 37 °C	PMID 22166247	Open record: L-asparaginase (PfA) variant K274E, L-asparagine
L-asparaginase (PfA)variant T53Q	Pyrococcus furiosus	L-asparagine	246.7	8.3	30	pH 8.2, 80 °C	PMID 22166247	Open record: L-asparaginase (PfA) variant T53Q, L-asparagine
L-asparaginase (PfA)variant T53Q	Pyrococcus furiosus	L-asparagine	35.2	7.52	4.72	pH 7.4, 37 °C	PMID 22166247	Open record: L-asparaginase (PfA) variant T53Q, L-asparagine
L-asparaginase (PfA)variant T53Q/K274E	Pyrococcus furiosus	L-asparagine	277.9	8.2	34	pH 8.2, 80 °C	PMID 22166247	Open record: L-asparaginase (PfA) variant T53Q/K274E, L-asparagine
L-asparaginase (PfA)variant T53Q/K274E	Pyrococcus furiosus	L-asparagine	23.8	5.41	4.4	pH 7.4, 37 °C	PMID 22166247	Open record: L-asparaginase (PfA) variant T53Q/K274E, L-asparagine
L-asparaginase (WoA)	Wolinella succinogenes	L-asparagine	97.8	0.0217	4.5 × 10³	37 °C	PMID 28139703	Open record: L-asparaginase (WoA), L-asparagine
L-asparaginase (WoA)	Wolinella succinogenes	L-glutamine	1.58	0.38	4.2	37 °C	PMID 28139703	Open record: L-asparaginase (WoA), L-glutamine
L-asparaginase (WoA)variant S121P	Wolinella succinogenes	L-asparagine	86.7	0.0236	3.7 × 10³	37 °C	PMID 28139703	Open record: L-asparaginase (WoA) variant S121P, L-asparagine
L-asparaginase (WoA)variant S121P	Wolinella succinogenes	L-glutamine	11.33	0.32	35.3	37 °C	PMID 28139703	Open record: L-asparaginase (WoA) variant S121P, L-glutamine
L-asparaginase II (EcA2)	Escherichia coli	L-asparagine	24	0.015	1.6 × 10³	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2), L-asparagine
L-asparaginase II (EcA2)	Escherichia coli	L-aspartic acid β-hydroxamate	29	0.035	830	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2), L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)	Escherichia coli	L-glutamine	0.33	3.5	0.094	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2), L-glutamine
L-asparaginase II (EcA2)variant G11L	Escherichia coli	L-aspartic acid β-hydroxamate	—	0.13	—	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G11L, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G11V	Escherichia coli	L-aspartic acid β-hydroxamate	0.11	0.07	1.6	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G11V, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57A	Escherichia coli	L-aspartic acid β-hydroxamate	15.4	0.069	220	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G57A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57A	Escherichia coli	L-glutamine	0.01	5.7	0.018	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G57A, L-glutamine
L-asparaginase II (EcA2)variant G57L	Escherichia coli	L-aspartic acid β-hydroxamate	0.2	0.082	2.4	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G57L, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57V	Escherichia coli	L-aspartic acid β-hydroxamate	0.86	0.05	17	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G57V, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G57V	Escherichia coli	L-glutamine	0.006	2.4	0.0025	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G57V, L-glutamine
L-asparaginase II (EcA2)variant G88A	Escherichia coli	L-aspartic acid β-hydroxamate	0.004	0.04	0.1	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G88A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant G88I	Escherichia coli	L-aspartic acid β-hydroxamate	—	0.05	—	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant G88I, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248A	Escherichia coli	L-asparagine	7	0.095	74	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248A, L-asparagine
L-asparaginase II (EcA2)variant N248A	Escherichia coli	L-aspartic acid β-hydroxamate	27	0.19	140	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248A	Escherichia coli	L-glutamine	0.0029	16	2 × 10⁻⁴	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248A, L-glutamine
L-asparaginase II (EcA2)variant N248D	Escherichia coli	L-aspartic acid β-hydroxamate	4.6	0.056	82	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248D, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248D	Escherichia coli	L-glutamine	0.0068	3.5	0.0019	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248D, L-glutamine
L-asparaginase II (EcA2)variant N248E	Escherichia coli	L-aspartic acid β-hydroxamate	26	0.14	190	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248E, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248E	Escherichia coli	L-glutamine	0.019	70	0.0027	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248E, L-glutamine
L-asparaginase II (EcA2)variant N248G	Escherichia coli	L-aspartic acid β-hydroxamate	23	0.21	110	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248G, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248G	Escherichia coli	L-glutamine	0.0046	6	8 × 10⁻⁴	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248G, L-glutamine
L-asparaginase II (EcA2)variant N248Q	Escherichia coli	L-aspartic acid β-hydroxamate	21	0.15	140	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248Q, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant N248Q	Escherichia coli	L-glutamine	0.032	21	0.0015	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant N248Q, L-glutamine
L-asparaginase II (EcA2)variant Q59A	Escherichia coli	L-aspartic acid β-hydroxamate	9.7	1.9	5.1	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant Q59A, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant Q59A	Escherichia coli	L-glutamine	0.001	10	1 × 10⁻⁴	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant Q59A, L-glutamine
L-asparaginase II (EcA2)variant Q59E	Escherichia coli	L-aspartic acid β-hydroxamate	2	0.037	54	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant Q59E, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant Q59E	Escherichia coli	L-glutamine	0.0024	23	0.001	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant Q59E, L-glutamine
L-asparaginase II (EcA2)variant Q59G	Escherichia coli	L-aspartic acid β-hydroxamate	14.3	1.8	7.9	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant Q59G, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant Q59G	Escherichia coli	L-glutamine	0.01	50	2 × 10⁻⁴	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant Q59G, L-glutamine
L-asparaginase II (EcA2)variant V27L	Escherichia coli	L-aspartic acid β-hydroxamate	10.3	0.011	940	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant V27L, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant V27L	Escherichia coli	L-glutamine	0.091	4.4	0.021	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant V27L, L-glutamine
L-asparaginase II (EcA2)variant V27M	Escherichia coli	L-aspartic acid β-hydroxamate	10.8	0.015	720	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant V27M, L-aspartic acid β-hydroxamate
L-asparaginase II (EcA2)variant V27M	Escherichia coli	L-glutamine	0.032	4	0.008	pH 7, 25 °C	PMID 11106175	Open record: L-asparaginase II (EcA2) variant V27M, L-glutamine
L-asparaginase II (EcAII, type II L-asparaginase)	Escherichia coli	L-asparagine	59.77	0.03	1975		PMID 29101342	Open record: L-asparaginase II (EcAII, type II L-asparaginase), L-asparagine
L-asparaginase II (EcAII, type II L-asparaginase)	Escherichia coli	L-glutamine	0.51	3.95	0.13		PMID 29101342	Open record: L-asparaginase II (EcAII, type II L-asparaginase), L-glutamine
L-asparaginase II (EcAII, type II L-asparaginase)variant N24S	Escherichia coli	L-asparagine	58.81	0.03	1782		PMID 29101342	Open record: L-asparaginase II (EcAII, type II L-asparaginase) variant N24S, L-asparagine
L-asparaginase II (EcAII, type II L-asparaginase)variant N24S	Escherichia coli	L-glutamine	0.53	4.14	0.13		PMID 29101342	Open record: L-asparaginase II (EcAII, type II L-asparaginase) variant N24S, L-glutamine
L-asparaginase II (type II L-asparaginase, Elspar)	Escherichia coli	L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme	60	—	—	pH 8.5, 37 °C	PMID 26142822	Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine
L-asparaginase II (type II L-asparaginase, Elspar)	Escherichia coli	L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme	72	—	—	pH 8.5, 37 °C	PMID 26142822	Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine
L-asparaginase II (type II L-asparaginase, Elspar)	Escherichia coli	L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme	67	—	—	pH 8.5, 37 °C	PMID 26142822	Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine
L-asparaginase II (type II L-asparaginase, Elspar)	Escherichia coli	L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme	48	—	—	pH 8.5, 37 °C	PMID 26142822	Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine
L-asparaginase II (type II L-asparaginase, Elspar)	Escherichia coli	L-asparagineLC-MS/MS time courses with 7.5 nM enzyme; in the text	72	0.019	3.8 × 10³ calc.	pH 8.5, 37 °C	PMID 26142822	Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine
L-asparaginase II (type II L-asparaginase, Elspar)	Escherichia coli	L-glutamineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme	2.2	—	—	pH 8.5, 37 °C	PMID 26142822	Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-glutamine
L-asparaginase II (type II L-asparaginase, Elspar)	Escherichia coli	L-glutamineLC-MS/MS time courses with 7.5 nM enzyme	2	1.9	1.1 calc.	pH 8.5, 37 °C	PMID 26142822	Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-glutamine
L-asparaginase ReAIV	Rhizobium etli	acrylamide	12.6	5.5	2.3 calc.		PMID 38456185	Open record: L-asparaginase ReAIV, acrylamide
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; no added Zn2+	428	1.7	246	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; + 1.0 µM Zn2+ (optimal)	543	1.3	407	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM Tris-HCl buffer pH 9.0; no added Zn2+	546	1.6	348	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM Tris-HCl buffer pH 9.0; + 1.0 µM Zn2+ (optimal)	770	1.5	526	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; 20 mM NaCl	174	1.6	110 calc.	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine	663	1.3	514	pH 9, 45 °C	PMID 38456185	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine	682	1.4	490 calc.	pH 9, 55 °C	PMID 38456185	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIVvariant K137A	Rhizobium etli	L-asparagineno added Zn2+	35	4.74	7	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAIV variant K137A, L-asparagine
L-asparaginase ReAIVvariant K137A	Rhizobium etli	L-asparagine+ 5 µM Zn2+ (optimal for the mutant, Methods 4.6)	158	2.3	70	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAIV variant K137A, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; no added Zn2+	327	2.6	126	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; + 2.5 µM Zn2+ (optimal)	392	1.9	203	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagineno added Zn2+	416	2.7	154	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine+ 2.5 µM Zn2+ (optimal)	603	2.1	294	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; 20 mM NaCl	341	3.9	87 calc.	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAVvariant K138A	Rhizobium etli	L-asparagineno added Zn2+	4.8	2.1	2.2	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAV variant K138A, L-asparagine
L-asparaginase ReAVvariant K138A	Rhizobium etli	L-asparagine+ 10 µM Zn2+ (optimal for the mutant, Methods 4.6)	6.5	0.5	14	pH 9, 37 °C	PMID 38456185	Open record: L-asparaginase ReAV variant K138A, L-asparagine
LlA (Lupinus luteus potassium-independent asparaginase)	Lupinus luteus	beta-L-aspartyl-L-leucine	0.813	0.136	5.98	pH 7.5	PMID 15265041	Open record: LlA (Lupinus luteus potassium-independent asparaginase), beta-L-aspartyl-L-leucine
LlA (Lupinus luteus potassium-independent asparaginase)	Lupinus luteus	L-asparagine	0.32	4.8	0.0667	pH 7.5	PMID 15265041	Open record: LlA (Lupinus luteus potassium-independent asparaginase), L-asparagine