Values CatLog read from this paper
Catalytic Role of the Substrate Defines Specificity of Therapeutic l-Asparaginase
PMID 26142822doi:10.1016/j.jmb.2015.06.017Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (9 values) from this paper: L-asparaginase II (type II L-asparaginase, Elspar) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-asparaginase II (type II L-asparaginase, Elspar) | Escherichia coli | L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme | 60 | — | — | pH 8.5, 37 °C | Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine |
| L-asparaginase II (type II L-asparaginase, Elspar) | Escherichia coli | L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme | 72 | — | — | pH 8.5, 37 °C | Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine |
| L-asparaginase II (type II L-asparaginase, Elspar) | Escherichia coli | L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme | 67 | — | — | pH 8.5, 37 °C | Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine |
| L-asparaginase II (type II L-asparaginase, Elspar) | Escherichia coli | L-asparagineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme | 48 | — | — | pH 8.5, 37 °C | Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine |
| L-asparaginase II (type II L-asparaginase, Elspar) | Escherichia coli | L-asparagineLC-MS/MS time courses with 7.5 nM enzyme; in the text | 72 | 0.019 | 3.8 × 10³ calc. | pH 8.5, 37 °C | Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-asparagine |
| L-asparaginase II (type II L-asparaginase, Elspar) | Escherichia coli | L-glutamineLC-MS/MS product assay; 5 nM (Asn) or 20 nM (Gln) enzyme | 2.2 | — | — | pH 8.5, 37 °C | Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-glutamine |
| L-asparaginase II (type II L-asparaginase, Elspar) | Escherichia coli | L-glutamineLC-MS/MS time courses with 7.5 nM enzyme | 2 | 1.9 | 1.1 calc. | pH 8.5, 37 °C | Open record: L-asparaginase II (type II L-asparaginase, Elspar), L-glutamine |