Values CatLog read from this paper

Biochemical characterization of L-asparaginase isoforms from Rhizobium etli-the boosting effect of zinc

PMID 38456185doi:10.3389/fchem.2024.1373312Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 17 records (47 values) from this paper: L-asparaginase ReAIV, L-asparaginase ReAV from Rhizobium etli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.5.1.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
L-asparaginase ReAIV	Rhizobium etli	acrylamide	12.6	5.5	2.3 calc.		Open record: L-asparaginase ReAIV, acrylamide
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; no added Zn2+	428	1.7	246	pH 9, 37 °C	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; + 1.0 µM Zn2+ (optimal)	543	1.3	407	pH 9, 37 °C	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM Tris-HCl buffer pH 9.0; no added Zn2+	546	1.6	348	pH 9, 37 °C	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM Tris-HCl buffer pH 9.0; + 1.0 µM Zn2+ (optimal)	770	1.5	526	pH 9, 37 °C	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; 20 mM NaCl	174	1.6	110 calc.	pH 9, 37 °C	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine	663	1.3	514	pH 9, 45 °C	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIV	Rhizobium etli	L-asparagine	682	1.4	490 calc.	pH 9, 55 °C	Open record: L-asparaginase ReAIV, L-asparagine
L-asparaginase ReAIVvariant K137A	Rhizobium etli	L-asparagineno added Zn2+	35	4.74	7	pH 9, 37 °C	Open record: L-asparaginase ReAIV variant K137A, L-asparagine
L-asparaginase ReAIVvariant K137A	Rhizobium etli	L-asparagine+ 5 µM Zn2+ (optimal for the mutant, Methods 4.6)	158	2.3	70	pH 9, 37 °C	Open record: L-asparaginase ReAIV variant K137A, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; no added Zn2+	327	2.6	126	pH 9, 37 °C	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; + 2.5 µM Zn2+ (optimal)	392	1.9	203	pH 9, 37 °C	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagineno added Zn2+	416	2.7	154	pH 9, 37 °C	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine+ 2.5 µM Zn2+ (optimal)	603	2.1	294	pH 9, 37 °C	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAV	Rhizobium etli	L-asparagine10 mM carbonate buffer pH 9.0; 20 mM NaCl	341	3.9	87 calc.	pH 9, 37 °C	Open record: L-asparaginase ReAV, L-asparagine
L-asparaginase ReAVvariant K138A	Rhizobium etli	L-asparagineno added Zn2+	4.8	2.1	2.2	pH 9, 37 °C	Open record: L-asparaginase ReAV variant K138A, L-asparagine
L-asparaginase ReAVvariant K138A	Rhizobium etli	L-asparagine+ 10 µM Zn2+ (optimal for the mutant, Methods 4.6)	6.5	0.5	14	pH 9, 37 °C	Open record: L-asparaginase ReAV variant K138A, L-asparagine