Values CatLog read from this paper

Hyperthermophilic asparaginase mutants with enhanced substrate affinity and antineoplastic activity: structural insights on their mechanism of action

PMID 22166247doi:10.1096/fj.11-191254Open in Browse

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CatLog read 8 records (24 values) from this paper: L-asparaginase (PfA) from Pyrococcus furiosus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.1.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
L-asparaginase (PfA)	Pyrococcus furiosus	L-asparagine	888.6	12.1	73	pH 8.2, 80 °C	Open record: L-asparaginase (PfA), L-asparagine
L-asparaginase (PfA)	Pyrococcus furiosus	L-asparagine	17.98	8.12	2.21	pH 7.4, 37 °C	Open record: L-asparaginase (PfA), L-asparagine
L-asparaginase (PfA)variant K274E	Pyrococcus furiosus	L-asparagine	199.2	2.1	96	pH 8.2, 80 °C	Open record: L-asparaginase (PfA) variant K274E, L-asparagine
L-asparaginase (PfA)variant K274E	Pyrococcus furiosus	L-asparagine	24.9	4.3	5.78	pH 7.4, 37 °C	Open record: L-asparaginase (PfA) variant K274E, L-asparagine
L-asparaginase (PfA)variant T53Q	Pyrococcus furiosus	L-asparagine	246.7	8.3	30	pH 8.2, 80 °C	Open record: L-asparaginase (PfA) variant T53Q, L-asparagine
L-asparaginase (PfA)variant T53Q	Pyrococcus furiosus	L-asparagine	35.2	7.52	4.72	pH 7.4, 37 °C	Open record: L-asparaginase (PfA) variant T53Q, L-asparagine
L-asparaginase (PfA)variant T53Q/K274E	Pyrococcus furiosus	L-asparagine	277.9	8.2	34	pH 8.2, 80 °C	Open record: L-asparaginase (PfA) variant T53Q/K274E, L-asparagine
L-asparaginase (PfA)variant T53Q/K274E	Pyrococcus furiosus	L-asparagine	23.8	5.41	4.4	pH 7.4, 37 °C	Open record: L-asparaginase (PfA) variant T53Q/K274E, L-asparagine