Values CatLog read from this paper
Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies
PMID 11239085doi:10.1093/protein/13.12.857Open in Browse
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CatLog read 12 records (36 values) from this paper: penicillin acylase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| penicillin acylase | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 16.2 | 0.0113 | 1.43 × 10³ | pH 7, 30 °C | Open record: penicillin acylase, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylase | Escherichia coli | Penicillin G | 42 | 0.007 | 6 × 10³ | pH 7, 30 °C | Open record: penicillin acylase, Penicillin G |
| penicillin acylase | Escherichia coli | Phenylacetamide | 50 | 0.16 | 310 | pH 7, 30 °C | Open record: penicillin acylase, Phenylacetamide |
| penicillin acylasevariant αF146A | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 0.015 | 0.055 | 0.3 | pH 7, 30 °C | Open record: penicillin acylase variant αF146A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant αF146A | Escherichia coli | Penicillin G | 2.3 | 0.141 | 23 | pH 7, 30 °C | Open record: penicillin acylase variant αF146A, Penicillin G |
| penicillin acylasevariant αF146A | Escherichia coli | Phenylacetamide | 1 | 1.7 | 5.8 | pH 7, 30 °C | Open record: penicillin acylase variant αF146A, Phenylacetamide |
| penicillin acylasevariant αF146L | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 0.038 | 0.025 | 1.5 | pH 7, 30 °C | Open record: penicillin acylase variant αF146L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant αF146L | Escherichia coli | Penicillin G | 25 | 0.147 | 410 | pH 7, 30 °C | Open record: penicillin acylase variant αF146L, Penicillin G |
| penicillin acylasevariant αF146L | Escherichia coli | Phenylacetamide | 4 | 2.3 | 1.7 | pH 7, 30 °C | Open record: penicillin acylase variant αF146L, Phenylacetamide |
| penicillin acylasevariant αF146Y | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 1.8 | 0.0048 | 375 | pH 7, 30 °C | Open record: penicillin acylase variant αF146Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant αF146Y | Escherichia coli | Penicillin G | 1.5 | 0.005 | 300 | pH 7, 30 °C | Open record: penicillin acylase variant αF146Y, Penicillin G |
| penicillin acylasevariant αF146Y | Escherichia coli | Phenylacetamide | 22 | 0.044 | 500 | pH 7, 30 °C | Open record: penicillin acylase variant αF146Y, Phenylacetamide |