EC 3.5.1.11: penicillin amidase

HydrolasesOpen in Browse

CatLog read 125 records for EC 3.5.1.11 from 9 papers, for 4 organisms. Names in these papers: penicillin acylase, penicillin amidase (penicillin amidohydrolase), penicillin acylase PAS2, penicillin G acylase (E. coli PA) and 4 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 76 records for EC 3.5.1.11 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.1.11 in Browse.

Papers

Papers with records for EC 3.5.1.11
Paper	Organisms	Records
The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase	Escherichia coli	34
doi:10.1016/j.enzmictec.2004.04.021	Escherichia coli	22
Substrate specificity of penicillin amidase from E. coli	Escherichia coli	14
Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies	Escherichia coli	12
Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263	Escherichia coli	10
doi:10.1016/j.catcom.2009.09.025	Alcaligenes faecalis	10
Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library	—	9
Poly-lysine supported cross-linked enzyme aggregates of penicillin G acylase and its application in synthesis of β-lactam antibiotics	Providencia rettgeri	9
Overexpression of penicillin V acylase from Streptomyces lavendulae and elucidation of its catalytic residues	Streptomyces lavendulae	5

Records

Records for EC 3.5.1.11 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
ACPGA001 PGA (penicillin G acylase)	—	6-nitro-3-(phenylacetamido)-benzoic acid	58.3	0.0083	7.02 × 10³		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), 6-nitro-3-(phenylacetamido)-benzoic acid
ACPGA001 PGA (penicillin G acylase)	—	Amoxycillin	56.2	0.1052	530		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Amoxycillin
ACPGA001 PGA (penicillin G acylase)	—	Ampicillin	49.5	0.0944	520		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Ampicillin
ACPGA001 PGA (penicillin G acylase)	—	Carbenicillin	63.7	0.0519	1.23 × 10³		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Carbenicillin
ACPGA001 PGA (penicillin G acylase)	—	Cephalexin	34.2	0.0255	1.35 × 10³		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Cephalexin
ACPGA001 PGA (penicillin G acylase)	—	Cephaloridine	66.9	0.0164	4.08 × 10³		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Cephaloridine
ACPGA001 PGA (penicillin G acylase)	—	Cephalothin	78.3	0.0156	5.02 × 10³		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Cephalothin
ACPGA001 PGA (penicillin G acylase)	—	Penicillin G	64.2	0.0046	1.396 × 10⁴		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Penicillin G
ACPGA001 PGA (penicillin G acylase)	—	Penicillin V	48.4	0.0393	1.23 × 10³		PMID 24338159	Open record: ACPGA001 PGA (penicillin G acylase), Penicillin V
penicillin acylase	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	16.2	0.0113	1.43 × 10³	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylase	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	16.2	0.015	1.08 × 10³	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylase	Escherichia coli	2-nitro-5-phenylacetamidobenzoic acid	20	0.015	1.33 × 10³	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, 2-nitro-5-phenylacetamidobenzoic acid
penicillin acylase	Escherichia coli	2-phenylacetamidobenzoic acid	2.3	0.63	4	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, 2-phenylacetamidobenzoic acid
penicillin acylase	Escherichia coli	3-phenylacetamidobenzoic acid	2.5	0.4	6	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, 3-phenylacetamidobenzoic acid
penicillin acylase	Escherichia coli	4-phenylacetamidobenzoic acid	3	0.3	10	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, 4-phenylacetamidobenzoic acid
penicillin acylase	Escherichia coli	amoxicillin	22	1.1	20	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, amoxicillin
penicillin acylase	Escherichia coli	ampicillin	30	2.5	12	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, ampicillin
penicillin acylase	Escherichia coli	cefadroxil	50	1	50	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, cefadroxil
penicillin acylase	Escherichia coli	cephalexin	57	1.2	47.5	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, cephalexin
penicillin acylase	Escherichia coli	D-phenylglycine amide	30	40	0.75	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, D-phenylglycine amide
penicillin acylase	Escherichia coli	D-phenylglycine methyl ester	50	12	4.16	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, D-phenylglycine methyl ester
penicillin acylase	Escherichia coli	N-(5-nitro-2-pyridyl)-phenylacetamide	7.8	0.51	15	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, N-(5-nitro-2-pyridyl)-phenylacetamide
penicillin acylase	Escherichia coli	p-hydroxy-D-phenylglycine amide	10.4	11.7	0.9	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, p-hydroxy-D-phenylglycine amide
penicillin acylase	Escherichia coli	p-hydroxy-D-phenylglycine methyl ester	16.2	12.5	1.3	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, p-hydroxy-D-phenylglycine methyl ester
penicillin acylase	Escherichia coli	Penicillin G	42	0.007	6 × 10³	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase, Penicillin G
penicillin acylase	Escherichia coli	penicillin G	50	0.01	5 × 10³	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, penicillin G
penicillin acylase	Escherichia coli	Phenylacetamide	50	0.16	310	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase, Phenylacetamide
penicillin acylase	Escherichia coli	phenylacetamide	50	0.2	250	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, phenylacetamide
penicillin acylase	Escherichia coli	phenylacetamide	50	—	250	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, phenylacetamide
penicillin acylase	Escherichia coli	phenylacetic acid methyl ester	190	0.16	1187	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase, phenylacetic acid methyl ester
penicillin acylase	Escherichia coli	phenylacetyl 2-naphthylamide	1.9	0.2	9.5	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, phenylacetyl 2-naphthylamide
penicillin acylase	Escherichia coli	phenylacetyl 4-nitroanilide	20	0.4	50	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, phenylacetyl 4-nitroanilide
penicillin acylase	Escherichia coli	phenylacetyl-4-methylcoumaryl-7-amide	3.2	0.012	270	pH 7, 25 °C	PMID 15153118	Open record: penicillin acylase, phenylacetyl-4-methylcoumaryl-7-amide
penicillin acylasevariant αF146A	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	0.015	0.055	0.3	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant αF146A	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	0.015	0.055	0.27	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant αF146A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant αF146A	Escherichia coli	Penicillin G	2.3	0.141	23	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146A, Penicillin G
penicillin acylasevariant αF146A	Escherichia coli	Phenylacetamide	1	1.7	5.8	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146A, Phenylacetamide
penicillin acylasevariant αF146L	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	0.038	0.025	1.5	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant αF146L	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	0.038	0.025	1.52	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant αF146L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant αF146L	Escherichia coli	Penicillin G	25	0.147	410	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146L, Penicillin G
penicillin acylasevariant αF146L	Escherichia coli	Phenylacetamide	4	2.3	1.7	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146L, Phenylacetamide
penicillin acylasevariant αF146W	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	1.1	0.032	34.4	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant αF146W, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant αF146Y	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	1.8	0.0048	375	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant αF146Y	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	1.6	0.005	320	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant αF146Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant αF146Y	Escherichia coli	D-phenylglycine amide	—	4.6	—	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant αF146Y, D-phenylglycine amide
penicillin acylasevariant αF146Y	Escherichia coli	Penicillin G	1.5	0.005	300	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146Y, Penicillin G
penicillin acylasevariant αF146Y	Escherichia coli	Phenylacetamide	22	0.044	500	pH 7, 30 °C	PMID 11239085	Open record: penicillin acylase variant αF146Y, Phenylacetamide
penicillin acylasevariant βF24A	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	1.6	0.275	6	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant βF24A	Escherichia coli	amoxicillin	16.1	11.3	1.42	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, amoxicillin
penicillin acylasevariant βF24A	Escherichia coli	ampicillin	3	1	3	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, ampicillin
penicillin acylasevariant βF24A	Escherichia coli	cefadroxil	5.2	0.76	6.87	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, cefadroxil
penicillin acylasevariant βF24A	Escherichia coli	cephalexin	9.8	2.8	3.48	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, cephalexin
penicillin acylasevariant βF24A	Escherichia coli	D-phenylglycine amide	2	25	0.08	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, D-phenylglycine amide
penicillin acylasevariant βF24A	Escherichia coli	D-phenylglycine methyl ester	27.7	8.7	3.18	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, D-phenylglycine methyl ester
penicillin acylasevariant βF24A	Escherichia coli	p-hydroxy-D-phenylglycine amide	2.8	28.7	0.097	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, p-hydroxy-D-phenylglycine amide
penicillin acylasevariant βF24A	Escherichia coli	p-hydroxy-D-phenylglycine methyl ester	20	5.9	3.4	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, p-hydroxy-D-phenylglycine methyl ester
penicillin acylasevariant βF24A	Escherichia coli	phenylacetamide	3.9	2	1.94	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, phenylacetamide
penicillin acylasevariant βF24A	Escherichia coli	phenylacetic acid methyl ester	7.5	2	3.75	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24A, phenylacetic acid methyl ester
penicillin acylasevariant βF24L	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	36	0.142	248	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant βF24W	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	1.5	0.101	15	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24W, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant βF24Y	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	12	0.351	34	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF24Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant βF57A	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	17	0.04	425	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF57A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant βF57L	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	24	0.049	490	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF57L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant βF57W	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	1.6	0.028	57	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF57W, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylasevariant βF57Y	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	0.7	0.32	2.2	pH 7, 30 °C	PMID 11985586	Open record: penicillin acylase variant βF57Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylase PAS2	—	2-nitro-5-[(phenylacetyl)amino]benzoic acid	24	0.004	6 × 10³	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin acylase PAS2	—	Amoxicillin	15	0.399	37.6	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, Amoxicillin
penicillin acylase PAS2	—	Ampicillin	16	0.575	27.8	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, Ampicillin
penicillin acylase PAS2	—	Cefadroxil	13	0.284	45.8	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, Cefadroxil
penicillin acylase PAS2	—	Cephalexin	20	1.3	15.4	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, Cephalexin
penicillin acylase PAS2	—	D-2-nitro-5-[(phenylglycyl)amino]benzoic acid	12	0.646	18.6	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, D-2-nitro-5-[(phenylglycyl)amino]benzoic acid
penicillin acylase PAS2	—	D-p-hydroxyphenylglycine amide	16	9.1	1.8	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, D-p-hydroxyphenylglycine amide
penicillin acylase PAS2	—	D-phenylglycine amide	25	12	2.1	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, D-phenylglycine amide
penicillin acylase PAS2	—	p-hydroxyphenylacetamide	29	0.027	1074	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, p-hydroxyphenylacetamide
penicillin acylase PAS2	—	Penicillin G	25	0.012	2083	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, Penicillin G
penicillin acylase PAS2	—	phenylacetamide	23	0.03	767	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin acylase PAS2, phenylacetamide
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	7-phenylacetamidodeacetoxycephalosporanic acid	50	0.01	5 × 10³	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), 7-phenylacetamidodeacetoxycephalosporanic acid
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Ampicillin	11	5.2	2.2	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Ampicillin
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Benzylpenicillin	48	0.0046	1 × 10⁴	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Benzylpenicillin
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Benzylpenicilloic acid	40	2	20	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Benzylpenicilloic acid
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Cephalexin	54	2.1	26	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Cephalexin
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Cephaloridine	33	0.1	330	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Cephaloridine
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Cephalothin	25	0.042	600	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Cephalothin
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	D-(-)-α-aminophenylacetic acid p-nitroanilide	0.54	3.2	0.157	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), D-(-)-α-aminophenylacetic acid p-nitroanilide
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	D-(-)-α-aminophenylacetic acid p-nitroanilide	—	—	0.22	25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), D-(-)-α-aminophenylacetic acid p-nitroanilide
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Ethyl phenylacetate	170	0.045	3.8 × 10³	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Ethyl phenylacetate
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	p-Nitrophenyl phenylacetate	170	0.031	5.5 × 10³	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), p-Nitrophenyl phenylacetate
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	phenylacetic acid p-nitroanilide	55	0.097	570	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), phenylacetic acid p-nitroanilide
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	phenylacetic acid p-nitroanilide	—	—	570	25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), phenylacetic acid p-nitroanilide
penicillin amidase (penicillin amidohydrolase)	Escherichia coli	Phenylacetylglycine	47	0.08	590	pH 7.5, 25 °C	PMID 7011386	Open record: penicillin amidase (penicillin amidohydrolase), Phenylacetylglycine
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	2-phenyl-N-(4-sulfamoylphenyl)acetamide	40.03	0.147	272	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), 2-phenyl-N-(4-sulfamoylphenyl)acetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	ethyl 4-[(phenylacetyl)amino]benzoate	41.05	0.153	268	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), ethyl 4-[(phenylacetyl)amino]benzoate
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N,2-diphenylacetamide	49.17	0.137	360	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N,2-diphenylacetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N-(4-acetylphenyl)-2-phenylacetamide	35.94	0.23	156	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N-(4-acetylphenyl)-2-phenylacetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N-(4-bromophenyl)-2-phenylacetamide	43.07	0.171	250	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N-(4-bromophenyl)-2-phenylacetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N-(4-cyanophenyl)-2-phenylacetamide	33.84	0.16	212	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N-(4-cyanophenyl)-2-phenylacetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N-(4-methoxyphenyl)-2-phenylacetamide	33.81	0.162	209	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N-(4-methoxyphenyl)-2-phenylacetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N-(4-methylphenyl)-2-phenylacetamide	37.93	0.121	314	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N-(4-methylphenyl)-2-phenylacetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N-(4-nitrophenyl)-2-phenylacetamide	15.14	0.095	159	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N-(4-nitrophenyl)-2-phenylacetamide
penicillin G acylase (Af-PGA)	Alcaligenes faecalis	N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide	29.49	0.159	185	pH 7, 25 °C	doi:10.1016/j.catcom.2009.09.025	Open record: penicillin G acylase (Af-PGA), N-[4-(methylsulfonyl)phenyl]-2-phenylacetamide
penicillin G acylase (E. coli PA)	Escherichia coli	2-nitro-5-[(phenylacetyl)amino]benzoic acid	18	0.015	1.2 × 10³	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin G acylase (E. coli PA)	Escherichia coli	Amoxicillin	17	1.07	15.9	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), Amoxicillin
penicillin G acylase (E. coli PA)	Escherichia coli	Ampicillin	25	2.5	10	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), Ampicillin
penicillin G acylase (E. coli PA)	Escherichia coli	Cefadroxil	32	0.642	49.8	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), Cefadroxil
penicillin G acylase (E. coli PA)	Escherichia coli	Cephalexin	29	1.5	19.3	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), Cephalexin
penicillin G acylase (E. coli PA)	Escherichia coli	D-2-nitro-5-[(phenylglycyl)amino]benzoic acid	14	1.3	10.8	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), D-2-nitro-5-[(phenylglycyl)amino]benzoic acid
penicillin G acylase (E. coli PA)	Escherichia coli	D-p-hydroxyphenylglycine amide	28	12.2	2.3	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), D-p-hydroxyphenylglycine amide
penicillin G acylase (E. coli PA)	Escherichia coli	D-phenylglycine amide	57	30	1.9	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), D-phenylglycine amide
penicillin G acylase (E. coli PA)	Escherichia coli	p-hydroxyphenylacetamide	47	0.114	412	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), p-hydroxyphenylacetamide
penicillin G acylase (E. coli PA)	Escherichia coli	Penicillin G	39	0.013	3 × 10³	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), Penicillin G
penicillin G acylase (E. coli PA)	Escherichia coli	phenylacetamide	46	0.156	295	pH 7, 30 °C	doi:10.1016/j.enzmictec.2004.04.021	Open record: penicillin G acylase (E. coli PA), phenylacetamide
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	2-nitro-5-[(phenylacetyl)amino]benzoic acid	17.6	0.9919	17.74	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	2-nitro-5-[(phenylacetyl)amino]benzoic acid	11.61	1.672	6.94	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	2-nitro-5-[(phenylacetyl)amino]benzoic acid	16.9	1.007	16.78	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), 2-nitro-5-[(phenylacetyl)amino]benzoic acid
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	Amoxicillin	15.7	30.39	0.52	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), Amoxicillin
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	Amoxicillin	11.73	51.15	0.22	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), Amoxicillin
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	Amoxicillin	14.67	41.26	0.356	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), Amoxicillin
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	D-hydroxyphenylglycine methyl ester	145.3	7.9	18.39	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), D-hydroxyphenylglycine methyl ester
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	D-hydroxyphenylglycine methyl ester	102.6	11.74	8.74	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), D-hydroxyphenylglycine methyl ester
penicillin G acylase (PrPGA)mutant of ref. 15 (mutation not named in this paper)	Providencia rettgeri	D-hydroxyphenylglycine methyl ester	142.3	7.31	19.47	pH 7, 30 °C	PMID 31381925	Open record: penicillin G acylase (PrPGA) mutant of ref. 15 (mutation not named in this paper), D-hydroxyphenylglycine methyl ester
penicillin V acylase (recombinant SlPVA)	Streptomyces lavendulae	3-hexenoyl-penicillin	3.33	1.39	2.4	pH 8, 40 °C	PMID 25501472	Open record: penicillin V acylase (recombinant SlPVA), 3-hexenoyl-penicillin
penicillin V acylase (recombinant SlPVA)	Streptomyces lavendulae	hexanoyl-penicillin	14.75	0.82	17.93	pH 8, 40 °C	PMID 25501472	Open record: penicillin V acylase (recombinant SlPVA), hexanoyl-penicillin
penicillin V acylase (recombinant SlPVA)	Streptomyces lavendulae	octanoyl-penicillin	35.58	0.14	244.1	pH 8, 40 °C	PMID 25501472	Open record: penicillin V acylase (recombinant SlPVA), octanoyl-penicillin
penicillin V acylase (recombinant SlPVA)	Streptomyces lavendulae	phenoxymethyl penicillin	77.92	3.68	21.17	pH 8, 40 °C	PMID 25501472	Open record: penicillin V acylase (recombinant SlPVA), phenoxymethyl penicillin
penicillin V acylase (recombinant SlPVA)	Streptomyces lavendulae	phenylacetyl penicillin	10.95	58.18	0.18	pH 8, 40 °C	PMID 25501472	Open record: penicillin V acylase (recombinant SlPVA), phenylacetyl penicillin