Values CatLog read from this paper
The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase
PMID 11985586doi:10.1046/j.1432-1033.2002.02857.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 34 records (100 values) from this paper: penicillin acylase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| penicillin acylase | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 16.2 | 0.015 | 1.08 × 10³ | pH 7, 30 °C | Open record: penicillin acylase, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylase | Escherichia coli | amoxicillin | 22 | 1.1 | 20 | pH 7, 30 °C | Open record: penicillin acylase, amoxicillin |
| penicillin acylase | Escherichia coli | ampicillin | 30 | 2.5 | 12 | pH 7, 30 °C | Open record: penicillin acylase, ampicillin |
| penicillin acylase | Escherichia coli | cefadroxil | 50 | 1 | 50 | pH 7, 30 °C | Open record: penicillin acylase, cefadroxil |
| penicillin acylase | Escherichia coli | cephalexin | 57 | 1.2 | 47.5 | pH 7, 30 °C | Open record: penicillin acylase, cephalexin |
| penicillin acylase | Escherichia coli | D-phenylglycine amide | 30 | 40 | 0.75 | pH 7, 30 °C | Open record: penicillin acylase, D-phenylglycine amide |
| penicillin acylase | Escherichia coli | D-phenylglycine methyl ester | 50 | 12 | 4.16 | pH 7, 30 °C | Open record: penicillin acylase, D-phenylglycine methyl ester |
| penicillin acylase | Escherichia coli | p-hydroxy-D-phenylglycine amide | 10.4 | 11.7 | 0.9 | pH 7, 30 °C | Open record: penicillin acylase, p-hydroxy-D-phenylglycine amide |
| penicillin acylase | Escherichia coli | p-hydroxy-D-phenylglycine methyl ester | 16.2 | 12.5 | 1.3 | pH 7, 30 °C | Open record: penicillin acylase, p-hydroxy-D-phenylglycine methyl ester |
| penicillin acylase | Escherichia coli | phenylacetamide | 50 | 0.2 | 250 | pH 7, 30 °C | Open record: penicillin acylase, phenylacetamide |
| penicillin acylase | Escherichia coli | phenylacetic acid methyl ester | 190 | 0.16 | 1187 | pH 7, 30 °C | Open record: penicillin acylase, phenylacetic acid methyl ester |
| penicillin acylasevariant αF146A | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 0.015 | 0.055 | 0.27 | pH 7, 30 °C | Open record: penicillin acylase variant αF146A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant αF146L | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 0.038 | 0.025 | 1.52 | pH 7, 30 °C | Open record: penicillin acylase variant αF146L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant αF146W | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 1.1 | 0.032 | 34.4 | pH 7, 30 °C | Open record: penicillin acylase variant αF146W, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant αF146Y | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 1.6 | 0.005 | 320 | pH 7, 30 °C | Open record: penicillin acylase variant αF146Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant αF146Y | Escherichia coli | D-phenylglycine amide | — | 4.6 | — | pH 7, 30 °C | Open record: penicillin acylase variant αF146Y, D-phenylglycine amide |
| penicillin acylasevariant βF24A | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 1.6 | 0.275 | 6 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant βF24A | Escherichia coli | amoxicillin | 16.1 | 11.3 | 1.42 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, amoxicillin |
| penicillin acylasevariant βF24A | Escherichia coli | ampicillin | 3 | 1 | 3 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, ampicillin |
| penicillin acylasevariant βF24A | Escherichia coli | cefadroxil | 5.2 | 0.76 | 6.87 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, cefadroxil |
| penicillin acylasevariant βF24A | Escherichia coli | cephalexin | 9.8 | 2.8 | 3.48 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, cephalexin |
| penicillin acylasevariant βF24A | Escherichia coli | D-phenylglycine amide | 2 | 25 | 0.08 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, D-phenylglycine amide |
| penicillin acylasevariant βF24A | Escherichia coli | D-phenylglycine methyl ester | 27.7 | 8.7 | 3.18 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, D-phenylglycine methyl ester |
| penicillin acylasevariant βF24A | Escherichia coli | p-hydroxy-D-phenylglycine amide | 2.8 | 28.7 | 0.097 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, p-hydroxy-D-phenylglycine amide |
| penicillin acylasevariant βF24A | Escherichia coli | p-hydroxy-D-phenylglycine methyl ester | 20 | 5.9 | 3.4 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, p-hydroxy-D-phenylglycine methyl ester |
| penicillin acylasevariant βF24A | Escherichia coli | phenylacetamide | 3.9 | 2 | 1.94 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, phenylacetamide |
| penicillin acylasevariant βF24A | Escherichia coli | phenylacetic acid methyl ester | 7.5 | 2 | 3.75 | pH 7, 30 °C | Open record: penicillin acylase variant βF24A, phenylacetic acid methyl ester |
| penicillin acylasevariant βF24L | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 36 | 0.142 | 248 | pH 7, 30 °C | Open record: penicillin acylase variant βF24L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant βF24W | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 1.5 | 0.101 | 15 | pH 7, 30 °C | Open record: penicillin acylase variant βF24W, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant βF24Y | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 12 | 0.351 | 34 | pH 7, 30 °C | Open record: penicillin acylase variant βF24Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant βF57A | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 17 | 0.04 | 425 | pH 7, 30 °C | Open record: penicillin acylase variant βF57A, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant βF57L | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 24 | 0.049 | 490 | pH 7, 30 °C | Open record: penicillin acylase variant βF57L, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant βF57W | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 1.6 | 0.028 | 57 | pH 7, 30 °C | Open record: penicillin acylase variant βF57W, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |
| penicillin acylasevariant βF57Y | Escherichia coli | 2-nitro-5-[(phenylacetyl)amino]benzoic acid | 0.7 | 0.32 | 2.2 | pH 7, 30 °C | Open record: penicillin acylase variant βF57Y, 2-nitro-5-[(phenylacetyl)amino]benzoic acid |