Values CatLog read from this paper
Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263
PMID 15153118doi:10.1111/j.1432-1033.2004.04170.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (29 values) from this paper: penicillin acylase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| penicillin acylase | Escherichia coli | 2-nitro-5-phenylacetamidobenzoic acid | 20 | 0.015 | 1.33 × 10³ | pH 7, 25 °C | Open record: penicillin acylase, 2-nitro-5-phenylacetamidobenzoic acid |
| penicillin acylase | Escherichia coli | 2-phenylacetamidobenzoic acid | 2.3 | 0.63 | 4 | pH 7, 25 °C | Open record: penicillin acylase, 2-phenylacetamidobenzoic acid |
| penicillin acylase | Escherichia coli | 3-phenylacetamidobenzoic acid | 2.5 | 0.4 | 6 | pH 7, 25 °C | Open record: penicillin acylase, 3-phenylacetamidobenzoic acid |
| penicillin acylase | Escherichia coli | 4-phenylacetamidobenzoic acid | 3 | 0.3 | 10 | pH 7, 25 °C | Open record: penicillin acylase, 4-phenylacetamidobenzoic acid |
| penicillin acylase | Escherichia coli | N-(5-nitro-2-pyridyl)-phenylacetamide | 7.8 | 0.51 | 15 | pH 7, 25 °C | Open record: penicillin acylase, N-(5-nitro-2-pyridyl)-phenylacetamide |
| penicillin acylase | Escherichia coli | penicillin G | 50 | 0.01 | 5 × 10³ | pH 7, 25 °C | Open record: penicillin acylase, penicillin G |
| penicillin acylase | Escherichia coli | phenylacetamide | 50 | — | 250 | pH 7, 25 °C | Open record: penicillin acylase, phenylacetamide |
| penicillin acylase | Escherichia coli | phenylacetyl 2-naphthylamide | 1.9 | 0.2 | 9.5 | pH 7, 25 °C | Open record: penicillin acylase, phenylacetyl 2-naphthylamide |
| penicillin acylase | Escherichia coli | phenylacetyl 4-nitroanilide | 20 | 0.4 | 50 | pH 7, 25 °C | Open record: penicillin acylase, phenylacetyl 4-nitroanilide |
| penicillin acylase | Escherichia coli | phenylacetyl-4-methylcoumaryl-7-amide | 3.2 | 0.012 | 270 | pH 7, 25 °C | Open record: penicillin acylase, phenylacetyl-4-methylcoumaryl-7-amide |