Values CatLog read from this paper
Substrate specificity of penicillin amidase from E. coli
PMID 7011386doi:10.1016/0005-2744(80)90145-xOpen in Browse
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CatLog read 14 records (38 values) from this paper: penicillin amidase (penicillin amidohydrolase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | 7-phenylacetamidodeacetoxycephalosporanic acid | 50 | 0.01 | 5 × 10³ | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), 7-phenylacetamidodeacetoxycephalosporanic acid |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Ampicillin | 11 | 5.2 | 2.2 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Ampicillin |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Benzylpenicillin | 48 | 0.0046 | 1 × 10⁴ | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Benzylpenicillin |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Benzylpenicilloic acid | 40 | 2 | 20 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Benzylpenicilloic acid |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Cephalexin | 54 | 2.1 | 26 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Cephalexin |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Cephaloridine | 33 | 0.1 | 330 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Cephaloridine |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Cephalothin | 25 | 0.042 | 600 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Cephalothin |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | D-(-)-α-aminophenylacetic acid p-nitroanilide | 0.54 | 3.2 | 0.157 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), D-(-)-α-aminophenylacetic acid p-nitroanilide |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | D-(-)-α-aminophenylacetic acid p-nitroanilide | — | — | 0.22 | 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), D-(-)-α-aminophenylacetic acid p-nitroanilide |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Ethyl phenylacetate | 170 | 0.045 | 3.8 × 10³ | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Ethyl phenylacetate |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | p-Nitrophenyl phenylacetate | 170 | 0.031 | 5.5 × 10³ | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), p-Nitrophenyl phenylacetate |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | phenylacetic acid p-nitroanilide | 55 | 0.097 | 570 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), phenylacetic acid p-nitroanilide |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | phenylacetic acid p-nitroanilide | — | — | 570 | 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), phenylacetic acid p-nitroanilide |
| penicillin amidase (penicillin amidohydrolase) | Escherichia coli | Phenylacetylglycine | 47 | 0.08 | 590 | pH 7.5, 25 °C | Open record: penicillin amidase (penicillin amidohydrolase), Phenylacetylglycine |