Values CatLog read from this paper
Adaptation of a thermophilic enzyme, 3-isopropylmalate dehydrogenase, to low temperatures
PMID 11297666doi:10.1093/protein/14.2.85Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (30 values) from this paper: 3-isopropylmalate dehydrogenase (IPMDH) from Thermus thermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-isopropylmalate dehydrogenase (IPMDH) | Thermus thermophilus | D-3-isopropylmalate | 2.36 | 0.00125 | 1.89 × 10³ calc. | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH) | Thermus thermophilus | D-3-isopropylmalate | 45.8 | 0.0089 | 5.2 × 10³ calc. | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH) | Thermus thermophilus | NAD+ | — | 0.0258 | — | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH) | Thermus thermophilus | NAD+ | — | 0.341 | — | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant I11V/V15I/N237D (P415) | Thermus thermophilus | D-3-isopropylmalate | 7.2 | 0.0013 | 5.5 × 10³ calc. | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant I11V/V15I/N237D (P415), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant I11V/V15I/N237D (P415) | Thermus thermophilus | D-3-isopropylmalate | 50.1 | 0.0096 | 5.2 × 10³ calc. | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant I11V/V15I/N237D (P415), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant I11V/V15I/N237D (P415) | Thermus thermophilus | NAD+ | — | 0.157 | — | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant I11V/V15I/N237D (P415), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant I11V/V15I/N237D (P415) | Thermus thermophilus | NAD+ | — | 0.917 | — | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant I11V/V15I/N237D (P415), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant S92F (T1155) | Thermus thermophilus | D-3-isopropylmalate | 2.41 | 0.001 | 2.4 × 10³ calc. | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant S92F (T1155), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant S92F (T1155) | Thermus thermophilus | D-3-isopropylmalate | 44.7 | 0.0078 | 5.7 × 10³ calc. | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant S92F (T1155), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant S92F (T1155) | Thermus thermophilus | NAD+ | — | 0.0149 | — | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant S92F (T1155), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant S92F (T1155) | Thermus thermophilus | NAD+ | — | 0.225 | — | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant S92F (T1155), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V126M (P215) | Thermus thermophilus | D-3-isopropylmalate | 17.7 | 0.0012 | 1.5 × 10⁴ calc. | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V126M (P215), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V126M (P215) | Thermus thermophilus | D-3-isopropylmalate | 100 | 0.01 | 1.0 × 10⁴ calc. | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V126M (P215), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V126M (P215) | Thermus thermophilus | NAD+ | — | 1.01 | — | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V126M (P215), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V126M (P215) | Thermus thermophilus | NAD+ | — | 3.56 | — | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V126M (P215), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V15I (P115) | Thermus thermophilus | D-3-isopropylmalate | 10.7 | 0.0024 | 4.5 × 10³ calc. | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V15I (P115), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V15I (P115) | Thermus thermophilus | D-3-isopropylmalate | 63.6 | 0.011 | 5.8 × 10³ calc. | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V15I (P115), D-3-isopropylmalate |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V15I (P115) | Thermus thermophilus | NAD+ | — | 0.16 | — | pH 8, 40 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V15I (P115), NAD+ |
| 3-isopropylmalate dehydrogenase (IPMDH)variant V15I (P115) | Thermus thermophilus | NAD+ | — | 0.82 | — | pH 8, 70 °C | Open record: 3-isopropylmalate dehydrogenase (IPMDH) variant V15I (P115), NAD+ |