Values CatLog read from this paper
His-391 of beta-galactosidase (Escherichia coli) promotes catalyses by strong interactions with the transition state
PMID 11310566doi:10.1139/o00-101Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (33 values) from this paper: beta-galactosidase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-galactosidase | Escherichia coli | o-nitrophenyl-beta-D-galactopyranoside | 620 | 0.13 | 4.77 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidase | Escherichia coli | p-nitrophenyl-beta-D-galactopyranoside | 90 | 0.041 | 2.25 × 10³ | pH 7, 25 °C | Open record: beta-galactosidase, p-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391E | Escherichia coli | o-nitrophenyl-beta-D-galactopyranosidepartially purified (~50% pure; ammonium sulfate fraction) | 2.76 | 5.8 | 0.48 | pH 7, 25 °C | Open record: beta-galactosidase variant H391E, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391E | Escherichia coli | o-nitrophenyl-beta-D-galactopyranoside | 1.8 | 6.9 | 0.26 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant H391E, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391E | Escherichia coli | o-nitrophenyl-beta-D-galactopyranosidewith 1 M methanol (acceptor) | 2.7 | — | — | pH 7, 25 °C | Open record: beta-galactosidase variant H391E, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391E | Escherichia coli | p-nitrophenyl-beta-D-galactopyranoside | 0.24 | 2.1 | 0.11 | pH 7, 25 °C | Open record: beta-galactosidase variant H391E, p-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391E | Escherichia coli | p-nitrophenyl-beta-D-galactopyranosidewith 1 M methanol (acceptor) | 0.18 | — | — | pH 7, 25 °C | Open record: beta-galactosidase variant H391E, p-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391F | Escherichia coli | o-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl, 1 mM MgSO4) | 0.22 | 11.2 | 0.02 | pH 7, 25 °C | Open record: beta-galactosidase variant H391F, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391F | Escherichia coli | o-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl, 1 mM MgSO4) | 0.2 | 10 | 0.020 calc. | pH 7, 25 °C | Open record: beta-galactosidase variant H391F, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391F | Escherichia coli | o-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl, 1 mM MgSO4) | 0.22 | — | — | pH 7, 25 °C | Open record: beta-galactosidase variant H391F, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391F | Escherichia coli | o-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl); approximate kcat | 0.005 | — | — | pH 7, 25 °C | Open record: beta-galactosidase variant H391F, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391F | Escherichia coli | p-nitrophenyl-beta-D-galactopyranoside | 0.009 | 7.9 | 0.0011 | pH 7, 25 °C | Open record: beta-galactosidase variant H391F, p-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391F | Escherichia coli | p-nitrophenyl-beta-D-galactopyranosidewith 1 M methanol (acceptor) | 0.006 | — | — | pH 7, 25 °C | Open record: beta-galactosidase variant H391F, p-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391K | Escherichia coli | o-nitrophenyl-beta-D-galactopyranoside | 0.028 | 9.8 | 0.0029 | pH 7, 25 °C | Open record: beta-galactosidase variant H391K, o-nitrophenyl-beta-D-galactopyranoside |
| beta-galactosidasevariant H391K | Escherichia coli | p-nitrophenyl-beta-D-galactopyranoside | 0.006 | 4.5 | 0.0013 | pH 7, 25 °C | Open record: beta-galactosidase variant H391K, p-nitrophenyl-beta-D-galactopyranoside |