CatLogenzyme kinetics catalog

EC 3.2.1.23: beta-galactosidase

HydrolasesOpen in Browse

CatLog read 426 records for EC 3.2.1.23 from 28 papers, for 14 organisms. Names in these papers: beta-galactosidase, β-galactosidase, Sulfolobus solfataricus beta-glycosidase (SsβG), ebg enzyme (evolved beta-galactosidase) and 21 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 428 records for EC 3.2.1.23 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.23 in Browse.

Papers

PaperOrganismsRecords
Developing promiscuous glycosidases for glycoside synthesis: residues W433 and E432 in Sulfolobus solfataricus beta-glycosidase are important glucoside- and galactoside-specificity determinantsSulfolobus solfataricus51
The beta-galactosidase (Escherichia coli) reaction is partly facilitated by interactions of His-540 with the C6 hydroxyl of galactoseEscherichia coli44
Determination of the roles of Glu-461 in beta-galactosidase (Escherichia coli) using site-specific mutagenesisEscherichia coli42
The catalytic consequences of experimental evolution. Transition-state structure during catalysis by the evolved beta-galactosidases of Escherichia coli (ebg enzymes) changed by a single mutational…Escherichia coli31
His-357 of beta-galactosidase (Escherichia coli) interacts with the C3 hydroxyl in the transition state and helps to mediate catalysisEscherichia coli27
Catalytic consequences of experimental evolution: catalysis by a 'third-generation' evolvant of the second beta-galactosidase of Escherichia coli, ebgabcde, and by ebgabcd, a 'second-generation'…Escherichia coli22
Catalysis by the large subunit of the second beta-galactosidase of Escherichia coli in the absence of the small subunitEscherichia coli21
The catalytic consequences of experimental evolution. Studies on the subunit structure of the second (ebg) beta-galactosidase of Escherichia coli, and on catalysis by ebgab, an experimental evolvant…Escherichia coli20
E461H-beta-galactosidase (Escherichia coli): altered divalent metal specificity and slow but reversible metal inactivationEscherichia coli20
His-391 of beta-galactosidase (Escherichia coli) promotes catalyses by strong interactions with the transition stateEscherichia coli15
Km defect in neuraminidase of dysmorphic type sialidosis with and without beta-galactosidase deficiencyHomo sapiens15
A new cold-adapted beta-D-galactosidase from the Antarctic Arthrobacter sp. 32c - gene cloning, overexpression, purification and propertiesArthrobacter sp.11
Trp-999 of beta-galactosidase (Escherichia coli) is a key residue for binding, catalysis, and synthesis of allolactose, the natural lac operon inducerEscherichia coli10
Characterization of the bga1-encoded glycoside hydrolase family 35 beta-galactosidase of Hypocrea jecorina with galacto-beta-D-galactanase activityHypocrea jecorina (Trichoderma reesei)10
Direct and indirect roles of His-418 in metal binding and in the activity of beta-galactosidase (E. coli)Escherichia coli10
Biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an Antarctic arthrobacter isolateArthrobacter sp., Escherichia coli9
One-step purification of a recombinant beta-galactosidase using magnetic cellulose as a support: Rapid immobilization and high thermal stabilityKluyveromyces sp.9
Effects of several alcohols on glycosidase activities in rat liver and brainRattus norvegicus8
Isolation and characterization of a new family 42 beta-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residuesAlicyclobacillus acidocaldarius8
A novel metagenome-derived beta-galactosidase: gene cloning, overexpression, purification and characterizationuncultured microbes8
Structural features of Aspergillus niger β-galactosidase define its activity against glycoside linkagesAspergillus niger, Aspergillus oryzae8
Probing the role of an invariant active site His in family GH1 β-glycosidasesSulfolobus solfataricus6
The C-terminal lectin-like domain modulates the substrate specificity and transglycosylation activity of rice β-galactosidase1 (OsBGal1)Oryza sativa5
DNA family shuffling of hyperthermostable beta-glycosidasesSulfolobus solfataricus4
Thermostable beta-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and propertiesSulfolobus solfataricus4
Cooperation between β-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzaeAspergillus oryzae4
Purification and properties of a beta-galactosidase with potential application as a digestive supplementKluyveromyces marxianus3
A discrete genetic locus confers xyloglucan metabolism in select human gut BacteroidetesBacteroides ovatus1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosideliver; Table 2—0.15—pH 4.5, 37 °CPMID 1499277Open
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosideliver; Table 2—0.19—pH 4.5, 37 °CPMID 1499277Open
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosideliver; Table 2—0.17—pH 4.5, 37 °CPMID 1499277Open
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosidebrain; Table 4—0.14—pH 4.5, 37 °CPMID 1499277Open
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosidebrain; Table 4—0.15—pH 4.5, 37 °CPMID 1499277Open
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosidebrain; Table 4—0.14—pH 4.5, 37 °CPMID 1499277Open
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosidebrain; Table 4—0.14—pH 4.5, 37 °CPMID 1499277Open
beta-D-galactosidaseRattus norvegicus4-nitrophenyl beta-D-galactopyranosidebrain; Table 4—0.14—pH 4.5, 37 °CPMID 1499277Open
beta-galactosidaseEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay7500.126.2 × 10³ calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidaseEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)82.50.62130 calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidaseEscherichia coli4-nitrophenyl beta-D-galactopyranoside900.0382.4 × 10³ calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidaseEscherichia colio-nitrophenyl beta-D-galactopyranoside1 mM Mg2+5800.124.8 × 10³ calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidaseEscherichia colio-nitrophenyl beta-D-galactopyranoside20 mM EDTA360.5763 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidaseEscherichia colio-nitrophenyl-beta-D-galactopyranoside6200.134.77 × 10³pH 7, 25 °CPMID 11310566Open
beta-galactosidaseEscherichia coliortho-nitrophenyl-β-D-galactopyranoside6000.125.0 × 10³ calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidaseEscherichia colip-nitrophenyl beta-D-galactopyranoside1 mM Mg2+980.0342.9 × 10³ calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidaseEscherichia colip-nitrophenyl beta-D-galactopyranosideEDTA (no Mg2+)130.12110 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidaseEscherichia colip-nitrophenyl-beta-D-galactopyranoside900.0412.25 × 10³pH 7, 25 °CPMID 11310566Open
beta-galactosidaseEscherichia colipara-nitrophenyl-β-D-galactopyranoside900.042.2 × 10³ calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.1——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.008—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.12——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.16——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461DEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)1.990.54.0 calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461DEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.007——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461DEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.011—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461DEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor0.007——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.71——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.008—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I18——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I29——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)0.1310.0245.5 calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.103——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.014—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461GEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor0.105——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I42——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.15—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I71——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)480.14340 calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia coli4-nitrophenyl beta-D-galactopyranoside2.1——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.074—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor2.2——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside100 mM EDTA; no Mg2+ added440.15290 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside20 mM EDTA; no Mg2+ added500.17290 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside1 mM Mg2+480.17280 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside5 mM Mg2+370.29130 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside10 mM Mg2+280.26110 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside20 mM Mg2+200.3853 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside50 mM Mg2+110.9312 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colio-nitrophenyl beta-D-galactopyranoside100 mM Mg2+5.71.83.2 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside100 mM EDTA; no Mg2+ added3.80.1329 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside20 mM EDTA; no Mg2+ added40.1527 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside1 mM Mg2+40.1822 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside5 mM Mg2+2.20.2210 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside10 mM Mg2+1.90.247.9 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside20 mM Mg2+1.60.44.0 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside50 mM Mg2+1.20.851.4 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461HEscherichia colip-nitrophenyl beta-D-galactopyranoside100 mM Mg2+1.520.75 calc.pH 7, 25 °CPMID 7577931Open
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.2——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.006—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I10——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I26——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461KEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)0.130.026.5 calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461KEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.24——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461KEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.008—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461KEscherichia coli4-nitrophenyl beta-D-galactopyranoside10 mM sodium azide added as nucleophilic acceptor2.6——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I0.36——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I—0.001—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I22.5——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosideenzyme desalted into assay buffer before assay; Table I52——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli2-nitrophenyl beta-D-galactopyranosidewith 10 mM EDTA (enzyme pre-equilibrated 30-60 min)0.0040.0320.12 calc.pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.34——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli4-nitrophenyl beta-D-galactopyranoside—0.003—pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant E461QEscherichia coli4-nitrophenyl beta-D-galactopyranoside100 mM sodium azide added as nucleophilic acceptor19——pH 7, 25 °CPMID 1969405Open
beta-galactosidasevariant H391EEscherichia colio-nitrophenyl-beta-D-galactopyranosidepartially purified (~50% pure; ammonium sulfate fraction)2.765.80.48pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391EEscherichia colio-nitrophenyl-beta-D-galactopyranoside1.86.90.26 calc.pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391EEscherichia colio-nitrophenyl-beta-D-galactopyranosidewith 1 M methanol (acceptor)2.7——pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391EEscherichia colip-nitrophenyl-beta-D-galactopyranoside0.242.10.11pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391EEscherichia colip-nitrophenyl-beta-D-galactopyranosidewith 1 M methanol (acceptor)0.18——pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391FEscherichia colio-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl, 1 mM MgSO4)0.2211.20.02pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391FEscherichia colio-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl, 1 mM MgSO4)0.2100.020 calc.pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391FEscherichia colio-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl, 1 mM MgSO4)0.22——pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391FEscherichia colio-nitrophenyl-beta-D-galactopyranosideTES assay buffer (30 mM TES, 145 mM NaCl); approximate kcat0.005——pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391FEscherichia colip-nitrophenyl-beta-D-galactopyranoside0.0097.90.0011pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391FEscherichia colip-nitrophenyl-beta-D-galactopyranosidewith 1 M methanol (acceptor)0.006——pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391KEscherichia colio-nitrophenyl-beta-D-galactopyranoside0.0289.80.0029pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant H391KEscherichia colip-nitrophenyl-beta-D-galactopyranoside0.0064.50.0013pH 7, 25 °CPMID 11310566Open
beta-galactosidasevariant W999FEscherichia coliortho-nitrophenyl-β-D-galactopyranoside540.26210 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant W999FEscherichia colipara-nitrophenyl-β-D-galactopyranoside670.43160 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant W999GEscherichia coliortho-nitrophenyl-β-D-galactopyranoside480.31150 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant W999GEscherichia colipara-nitrophenyl-β-D-galactopyranoside520.52100 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant W999LEscherichia coliortho-nitrophenyl-β-D-galactopyranoside203.36.1 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant W999LEscherichia colipara-nitrophenyl-β-D-galactopyranoside282.113 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant W999YEscherichia coliortho-nitrophenyl-β-D-galactopyranoside562.127 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidasevariant W999YEscherichia colipara-nitrophenyl-β-D-galactopyranoside651.641 calc.pH 7, 25 °CPMID 12578395Open
beta-galactosidaseHomo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.253—PMID 6811161Open
beta-galactosidasevariant patient cell line A.R. (sialidosis 2 juvenile onset (dysmorphic); mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.225—PMID 6811161Open
beta-galactosidasevariant patient cell line E.H. (mucolipidosis II; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.268—PMID 6811161Open
beta-galactosidasevariant patient cell line F.A. (mucolipidosis II; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.269—PMID 6811161Open
beta-galactosidasevariant patient cell line GM 1494 (mucolipidosis III; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.23—PMID 6811161Open
beta-galactosidasevariant patient cell line GM 1718 (sialidosis 2 infantile onset (dysmorphic); mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.232—PMID 6811161Open
beta-galactosidasevariant patient cell line GM 2048 (mucolipidosis IV; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.229—PMID 6811161Open
beta-galactosidasevariant patient cell line GM 2527 (mucolipidosis IV; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.255—PMID 6811161Open
beta-galactosidasevariant patient cell line GM 2629 (mucolipidosis IV; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.247—PMID 6811161Open
beta-galactosidasevariant patient cell line GM 2685 (sialidosis 2 infantile onset (dysmorphic); mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.278—PMID 6811161Open
beta-galactosidasevariant patient cell line M.L. (sialidosis 1 (normomorphic); mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.247—PMID 6811161Open
beta-galactosidasevariant patient cell line N.G. (mucolipidosis II; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.225—PMID 6811161Open
beta-galactosidasevariant patient cell line T.R. (GM1 gangliosidosis II; mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.245—PMID 6811161Open
beta-galactosidasevariant patient cell line [15] (sialidosis 1 (normomorphic); mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.301—PMID 6811161Open
beta-galactosidasevariant patient cell line [16] (sialidosis 2 juvenile onset (dysmorphic); mutation not characterized)Homo sapiens4-methylumbelliferyl beta-D-galactopyranoside—0.296—PMID 6811161Open
beta-galactosidaseKluyveromyces sp.2-nitrophenyl beta-D-galactopyranoside0.065811.040.00596 calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.2-nitrophenyl beta-D-galactopyranoside0.055211.130.00496 calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.2-nitrophenyl beta-D-galactopyranoside0.059215.180.00390 calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.lactose50 mM sodium phosphate buffer with 3 mM MgCl20.147572.40.00204 calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.lactose50 mM sodium phosphate buffer with 3 mM MgCl20.122372.920.001680 calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.lactose50 mM sodium phosphate buffer with 3 mM MgCl20.12482.220.00151 calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.lactose0.0382259.61.47 × 10⁻⁴ calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.lactose0.0588147.83.98 × 10⁻⁴ calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseKluyveromyces sp.lactose0.043209.52.0 × 10⁻⁴ calc.pH 7.5, 41 °CPMID 34883192Open
beta-galactosidaseSulfolobus solfataricus2-nitrophenyl beta-D-galactopyranoside950.225422.2pH 6.5, 75 °CPMID 2105216Open
beta-galactosidaseSulfolobus solfataricusalpha-lactose1.9130.145pH 6.5, 30 °CPMID 2105216Open
beta-galactosidaseSulfolobus solfataricusmethyl beta-D-galactopyranoside4.03230.175pH 6.5, 30 °CPMID 2105216Open
beta-galactosidaseSulfolobus solfataricusphenyl beta-D-galactopyranoside2.772.61.063pH 6.5, 30 °CPMID 2105216Open
beta-galactosidase (Aabeta-gal)Alicyclobacillus acidocaldarius2-nitrophenyl beta-D-fucopyranoside13457.3186pH 5.5, 65 °CPMID 18068682Open
beta-galactosidase (Aabeta-gal)Alicyclobacillus acidocaldarius2-nitrophenyl beta-D-galactopyranoside26575.5484pH 5.5, 65 °CPMID 18068682Open
beta-galactosidase (Aabeta-gal)Alicyclobacillus acidocaldarius4-nitrophenyl alpha-L-arabinopyranoside1.74 × 10³5.2332pH 5.5, 65 °CPMID 18068682Open
beta-galactosidase (Aabeta-gal)Alicyclobacillus acidocaldarius4-nitrophenyl beta-D-galactopyranoside29884.9609pH 5.5, 65 °CPMID 18068682Open
beta-galactosidase (Aabeta-gal)Alicyclobacillus acidocaldarius4-O-beta-D-galactopyranosyl-D-fructofuranose38018.221PMID 18068682Open
beta-galactosidase (Aabeta-gal)Alicyclobacillus acidocaldariuslactose21260.83PMID 18068682Open
beta-galactosidase (Aabeta-gal)variant E157GAlicyclobacillus acidocaldarius2-nitrophenyl beta-D-galactopyranoside0.320.112.9pH 5.5, 65 °CPMID 18068682Open
beta-galactosidase (Aabeta-gal)variant E313GAlicyclobacillus acidocaldarius2-nitrophenyl beta-D-galactopyranoside0.214.80.04pH 5.5, 65 °CPMID 18068682Open
beta-galactosidase (AnbGal)Aspergillus nigerlactose214.992.52.3pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (AnbGal)variant W806FAspergillus nigerlactose63.748.41.3pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (AnbGal)variant W806SAspergillus nigerlactose21.693.60.2pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (AnbGal)variant Y304AAspergillus nigerlactose123.915190.1pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (AnbGal)variant Y304FAspergillus nigerlactose277.389.53.1pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (AnbGal)variant Y304F/Y355H/N357GAspergillus nigerlactose212.196.52.2pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (AnbGal)variant Y304F/Y355H/N357G/W806FAspergillus nigerlactose128.3132.71pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (AobGal)Aspergillus oryzaelactose85.5118.40.8pH 3.5, 40 °CPMID 28391618Open
beta-galactosidase (intracellular)Kluyveromyces marxianus2-nitrophenyl beta-D-galactopyranoside85594.71.9 × 10³pH 6.8PMID 17625262Open
beta-galactosidase (intracellular)Kluyveromyces marxianus4-nitrophenyl beta-D-galactopyranoside2.7 × 10⁶7.13.9 × 10⁵pH 6.8PMID 17625262Open
beta-galactosidase (intracellular)Kluyveromyces marxianuslactose6.3 × 10³41.58 × 10³pH 6.8PMID 17625262Open
beta-galactosidase (LacZ)Escherichia coli2,3-dinitrophenyl beta-D-galactopyranoside1.19 × 10³0.157.93 × 10³pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)Escherichia coli2,5-dinitrophenyl beta-D-galactopyranoside1300.41315pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside1500.16915pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)Escherichia colio-nitrophenyl beta-D-galactopyranosideTES assay buffer: 30 mM TES; 1 mM MgSO46200.144.43 × 10³pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)Escherichia colio-nitrophenyl beta-D-galactopyranoside30 mM TES; no added Mg2+ (10 mM EDTA instead of 1 mM MgSO4)39——pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)Escherichia colip-nitrophenyl beta-D-galactopyranoside900.0412.2 × 10³pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357DEscherichia coli2,3-dinitrophenyl beta-D-galactopyranoside1040.0791.31 × 10³pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357DEscherichia coli2,5-dinitrophenyl beta-D-galactopyranoside2100.23920pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357DEscherichia coli3,4-dinitrophenyl beta-D-galactopyranoside4.30.009480pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357DEscherichia colio-nitrophenyl beta-D-galactopyranoside7.80.1359pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357DEscherichia colip-nitrophenyl beta-D-galactopyranoside0.10.00333pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357FEscherichia coli2,3-dinitrophenyl beta-D-galactopyranoside520.8363pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357FEscherichia coli2,5-dinitrophenyl beta-D-galactopyranoside3200.49660pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357FEscherichia coli3,4-dinitrophenyl beta-D-galactopyranoside11.40.073156pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357FEscherichia colio-nitrophenyl beta-D-galactopyranosideTES assay buffer: 30 mM TES; 1 mM MgSO4161.2513pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357FEscherichia colio-nitrophenyl beta-D-galactopyranoside30 mM TES; no added Mg2+ (10 mM EDTA instead of 1 mM MgSO4)1.1——pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357FEscherichia colip-nitrophenyl beta-D-galactopyranoside2.70.08332.5pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357LEscherichia coli2,3-dinitrophenyl beta-D-galactopyranoside13.10.11120pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357LEscherichia coli2,5-dinitrophenyl beta-D-galactopyranoside10.50.2542pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357LEscherichia coli3,4-dinitrophenyl beta-D-galactopyranoside4.50.06174pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357LEscherichia colio-nitrophenyl beta-D-galactopyranoside5.10.4911pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357LEscherichia colip-nitrophenyl beta-D-galactopyranoside0.40.00312pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357NEscherichia coli2,3-dinitrophenyl beta-D-galactopyranoside1.3 × 10³0.26.5 × 10³pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357NEscherichia coli2,5-dinitrophenyl beta-D-galactopyranoside1.89 × 10³0.573.33 × 10³pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357NEscherichia coli3,4-dinitrophenyl beta-D-galactopyranoside700.0145.01 × 10³pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357NEscherichia colio-nitrophenyl beta-D-galactopyranoside640.21290pH 7, 25 °CPMID 9665715Open
beta-galactosidase (LacZ)variant H357NEscherichia colip-nitrophenyl beta-D-galactopyranoside0.80.006130pH 7, 25 °CPMID 9665715Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)1,4-O-beta-D-galactopyranosyl-D-glucose0.698.790.078 calc.pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)2-nitrophenyl beta-D-galactopyranoside17.310.3648 calc.pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)4-O-beta-D-galactopyranosyl-D-fructose1.520.562.7 calc.pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)4-O-beta-D-galactopyranosyl-D-galactose5.689.060.627 calc.pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)4-O-beta-D-galactopyranosyl-D-glucitol0.1319.770.0066 calc.pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)4-O-beta-D-galactopyranosyl-D-gluconic acid0.115.310.0065 calc.pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)beta-1,3/beta-1,6-arabinogalactan from larch wood (79% Gal)4.34——pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)beta-1,4-galactan from lupin (91% Gal)6.87——pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)beta-1,4-galactan from potato (87% Gal)3.32——pH 5, 30 °CPMID 17381511Open
beta-galactosidase Bga1Hypocrea jecorina (Trichoderma reesei)methyl beta-D-galactopyranoside0.092.850.032 calc.pH 5, 30 °CPMID 17381511Open
beta-galactosidase ZD410uncultured microbes2-nitrophenyl beta-D-galactopyranoside23.76.33.7pH 7, 5 °CPMID 20614217Open
beta-galactosidase ZD410uncultured microbes2-nitrophenyl beta-D-galactopyranoside66.74.714.2pH 7, 10 °CPMID 20614217Open
beta-galactosidase ZD410uncultured microbes2-nitrophenyl beta-D-galactopyranoside1493.443.7pH 7, 20 °CPMID 20614217Open
beta-galactosidase ZD410uncultured microbes2-nitrophenyl beta-D-galactopyranoside3861.71226pH 7, 38 °CPMID 20614217Open
beta-galactosidase ZD410uncultured microbesLactose5358.60.9pH 7, 5 °CPMID 20614217Open
beta-galactosidase ZD410uncultured microbesLactose3327.11.21pH 7, 10 °CPMID 20614217Open
beta-galactosidase ZD410uncultured microbesLactose9217.65.23pH 7, 20 °CPMID 20614217Open
beta-galactosidase ZD410uncultured microbesLactose14112.211.6pH 7, 38 °CPMID 20614217Open
BgaS (beta-galactosidase)Arthrobacter sp.lactose4.719.40.275 °CPMID 12949099Open
BgaS (beta-galactosidase)Arthrobacter sp.lactose5.211.50.5318 °CPMID 12949099Open
BgaS (beta-galactosidase)Arthrobacter sp.o-nitrophenyl-beta-D-galactopyranoside430.761.45 °CPMID 12949099Open
BgaS (beta-galactosidase)Arthrobacter sp.o-nitrophenyl-beta-D-galactopyranoside57.20.781.710 °CPMID 12949099Open
BgaS (beta-galactosidase)Arthrobacter sp.o-nitrophenyl-beta-D-galactopyranoside1000.8116.318 °CPMID 12949099Open
BgaS (beta-galactosidase)Arthrobacter sp.o-nitrophenyl-beta-D-galactopyranosideN-terminal six-histidine fusion (H-BgaS); pET28a—0.6183.620 °CPMID 12949099Open
BgaS (beta-galactosidase)Arthrobacter sp.o-nitrophenyl-beta-D-galactopyranoside583.50.3182320 °CPMID 12949099Open
BoGH2A (β-galactosidase)Bacteroides ovatus4-nitrophenyl beta-D-galactopyranoside40.0874637 °CPMID 24463512Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)-3-bromopyridinium2.20.415.4 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)-3-chloropyridinium2.30.366.4 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)-3-methylpyridinium0.041.10.036 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli1-(beta-D-galactopyranosyl)pyridinium0.0961.60.060 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-(beta-D-galactopyranosyl)-4-bromoisoquinolinium0.780.352.2 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-(beta-D-galactopyranosyl)isoquinolinium0.0481.40.034 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-naphthyl beta-D-galactopyranoside3.910.080.39 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli2-nitrophenyl beta-D-galactopyranoside13.53.084.38 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside14.81.0314.4 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli3,5-dinitrophenyl beta-D-galactopyranoside15.11.2612.0 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli3-nitrophenyl beta-D-galactopyranoside14.37.12.0 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli4-bromophenyl beta-D-galactopyranoside7.517.150.44 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli4-cyanophenyl beta-D-galactopyranoside9.76.621.5 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coli4-nitrophenyl beta-D-galactopyranoside15.82.995.28 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebga evolvant (class I)Escherichia coliphenyl beta-D-galactopyranoside10.316.60.620 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3,5-dimethylpyridinium0.0451.90.024 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3-bromopyridinium5.60.3317 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3-chloropyridinium5.30.3814 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)-3-methylpyridinium0.041.50.027 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli1-(beta-D-galactopyranosyl)pyridinium0.213.10.068 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli2-(beta-D-galactopyranosyl)-4-bromoisoquinolinium8.30.4817 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli2-(beta-D-galactopyranosyl)isoquinolinium0.0720.790.091 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli2-naphthyl beta-D-galactopyranoside10.30.7314 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside11.60.89113.0 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3,5-dinitrophenyl beta-D-galactopyranoside12.10.16772.5 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3-methylphenyl beta-D-galactopyranoside5.71.713.3 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli3-nitrophenyl beta-D-galactopyranoside11.10.33233.4 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli4-bromophenyl beta-D-galactopyranoside8.81.028.6 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli4-cyanophenyl beta-D-galactopyranoside8.40.4519 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coli4-nitrophenyl beta-D-galactopyranoside10.50.26839.2 calc.pH 7.5, 25 °CPMID 2505746Open
ebg enzyme (evolved beta-galactosidase)ebgb evolvant (class II; W988C)Escherichia coliphenyl beta-D-galactopyranoside9.31.715.4 calc.pH 7.5, 25 °CPMID 2505746Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli1-(β-D-galactopyranosyl)pyridinium0.122.30.052 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli2,4,6-trinitrophenyl 1-thio-β-D-galactopyranoside2.80.2611 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli2,4-dinitrophenyl 1-thio-β-D-galactopyranoside0.560.163.5 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli2-(β-D-galactopyranosyl)isoquinolinium0.112.30.048 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli2-naphthyl β-D-galactopyranoside1.50.188.3 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli2-nitrophenyl β-D-galactopyranoside40.05671 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3,4-dinitrophenyl β-D-galactopyranoside3.30.04770 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3,4-dinitrophenyl β-D-galactopyranoside10.20.049210 calc.pH 7.5, 37 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3,5-dinitrophenyl β-D-galactopyranoside3.90.08248 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3-bromo-1-(β-D-galactopyranosyl)pyridinium40.3811 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3-chloro-1-(β-D-galactopyranosyl)pyridinium3.10.349.1 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3-methyl-1-(β-D-galactopyranosyl)pyridinium0.0220.70.031 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3-methylphenyl β-D-galactopyranoside1.50.324.7 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli3-nitrophenyl β-D-galactopyranoside4.40.2816 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli4-bromo-2-(β-D-galactopyranosyl)isoquinolinium30.2214 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli4-bromophenyl β-D-galactopyranoside2.70.2113 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli4-cyanophenyl β-D-galactopyranoside1.70.218.1 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coli4-nitrophenyl β-D-galactopyranoside3.10.131 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coliD-galactose16——pH 7.5, 37 °CPMID 1540130Open
ebg β-galactosidasevariant D92N/W977CEscherichia coliphenyl β-D-galactopyranoside1.20.641.9 calc.pH 7.5, 25 °CPMID 1540130Open
ebg β-galactosidase large subunit (ebga large subunit)variant D92NEscherichia coli3,4-dinitrophenyl β-D-galactopyranoside0.60.03617pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebga large subunit)variant D92NEscherichia coli3,5-dinitrophenyl β-D-galactopyranoside0.810.04517pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebga large subunit)variant D92NEscherichia coli3-methylphenyl β-D-galactopyranoside0.292.60.11pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebga large subunit)variant D92NEscherichia coli3-nitrophenyl β-D-galactopyranoside0.660.441.5pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebga large subunit)variant D92NEscherichia coli4-bromophenyl β-D-galactopyranoside0.512.20.22pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebga large subunit)variant D92NEscherichia coli4-nitrophenyl β-D-galactopyranoside0.610.431.4pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebga large subunit)variant D92NEscherichia coliphenyl β-D-galactopyranoside0.255.10.044pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebg° large subunit)Escherichia coli3,4-dinitrophenyl β-D-galactopyranoside0.760.04416pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebg° large subunit)Escherichia coli3,5-dinitrophenyl β-D-galactopyranoside0.90.04822pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebg° large subunit)Escherichia coli3-methylphenyl β-D-galactopyranoside0.425.90.077pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebg° large subunit)Escherichia coli3-nitrophenyl β-D-galactopyranoside0.720.720.98pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebg° large subunit)Escherichia coli4-bromophenyl β-D-galactopyranoside0.813.40.23pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebg° large subunit)Escherichia coli4-nitrophenyl β-D-galactopyranoside0.670.351.8pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ebg° large subunit)Escherichia coliphenyl β-D-galactopyranoside0.419.60.041pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ψebg° large subunit)variant L9HEscherichia coli3,4-dinitrophenyl β-D-galactopyranoside0.0980.0392.4pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ψebg° large subunit)variant L9HEscherichia coli3,5-dinitrophenyl β-D-galactopyranoside0.120.0422.7pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ψebg° large subunit)variant L9HEscherichia coli3-methylphenyl β-D-galactopyranoside0.0647.50.0097pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ψebg° large subunit)variant L9HEscherichia coli3-nitrophenyl β-D-galactopyranoside0.0960.490.2pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ψebg° large subunit)variant L9HEscherichia coli4-bromophenyl β-D-galactopyranoside0.0862.40.04pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ψebg° large subunit)variant L9HEscherichia coli4-nitrophenyl β-D-galactopyranoside0.0950.290.34pH 7.5, 25 °CPMID 7492325Open
ebg β-galactosidase large subunit (ψebg° large subunit)variant L9HEscherichia coliphenyl β-D-galactopyranoside0.0365.40.0065pH 7.5, 25 °CPMID 7492325Open
LacA (beta-galactosidase)Aspergillus oryzaeLactose8691048.36pH 4.5, 60 °CPMID 30980597Open
LacA (beta-galactosidase)Aspergillus oryzaeLG2892.41120pH 4.5, 60 °CPMID 30980597Open
LacA (beta-galactosidase)Aspergillus oryzaep-nitrophenyl beta-D-galactopyranoside5251.42370pH 4.5, 60 °CPMID 30980597Open
LacA (beta-galactosidase)Aspergillus oryzaeXLLG651.7637pH 4.5, 60 °CPMID 30980597Open
LacSSulfolobus solfataricuscellobiose23.213.51.72pH 5, 70 °CPMID 12164784Open
LacSSulfolobus solfataricuslactose12137.93.19pH 5, 70 °CPMID 12164784Open
LacSSulfolobus solfataricusp-nitrophenyl-β-D-galactopyranoside1651.57105pH 5, 70 °CPMID 12164784Open
LacSSulfolobus solfataricusp-nitrophenyl-β-D-glucopyranoside450.12375pH 5, 70 °CPMID 12164784Open
LacZ (beta-galactosidase)Escherichia colio-nitrophenyl-beta-D-galactopyranosideN-terminal six-histidine fusion (H-LacZ); pET28a31.70.216620 °CPMID 12949099Open
LacZ (beta-galactosidase)Escherichia colio-nitrophenyl-beta-D-galactopyranoside219.60.2109820 °CPMID 12949099Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli2-nitrophenyl beta-D-galactopyranoside4.30.021200 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside3.90.028140 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside8.60.033260 calc.pH 7.5, 37 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli3,5-dinitrophenyl beta-D-galactopyranoside4.50.023200 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli3-nitrophenyl beta-D-galactopyranoside40.220 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli4-bromophenyl beta-D-galactopyranoside4.40.08254 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli4-cyanophenyl beta-D-galactopyranoside3.60.1721 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coli4-nitrophenyl beta-D-galactopyranoside4.10.05575 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coliD-galactose13——pH 7.5, 37 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/E93K/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcde]Escherichia coliphenyl beta-D-galactopyranoside2.30.337.0 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli1-fluoro-D-galactopyranosyl fluoride0.1140.0851.3 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli2-nitrophenyl beta-D-galactopyranoside11.30.087130 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside9.040.052170 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli3,4-dinitrophenyl beta-D-galactopyranoside21.90.095230 calc.pH 7.5, 37 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli3,5-dinitrophenyl beta-D-galactopyranoside10.50.046230 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli3-nitrophenyl beta-D-galactopyranoside10.80.1290 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli4-bromophenyl beta-D-galactopyranoside9.340.1658 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli4-cyanophenyl beta-D-galactopyranoside11.10.1110 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coli4-nitrophenyl beta-D-galactopyranoside10.10.1192 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia colibeta-D-galactopyranosyl fluoride90.6414 calc.pH 7.5, 25 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coliD-galactose13——pH 7.5, 37 °CPMID 8554546Open
second beta-galactosidase (ebg), alpha4beta4 holoenzyme of ebgA and ebgC productsvariant D92N/W977C/S979G (large subunit EbgA) + E122G (small subunit EbgC) [evolvant ebg-abcd]Escherichia coliphenyl beta-D-galactopyranoside7.050.3520 calc.pH 7.5, 25 °CPMID 8554546Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-methylumbelliferyl beta-D-fucopyranoside800.0117.3 × 10³pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-methylumbelliferyl beta-D-galactopyranoside980.0661.49 × 10³pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-methylumbelliferyl beta-D-glucopyranoside1400.0462.9 × 10³pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-methylumbelliferyl beta-D-glucuronide0.811.30.6pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-methylumbelliferyl beta-D-mannopyranoside1.80.03650pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-methylumbelliferyl beta-D-xylopyranoside3.80.1330pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-fucopyranoside5.160.2142.64pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosidebuffer; Table 27.20.5712.74pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 324.665.064.873pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 319.732.817.021pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 317.582.96.061pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 312.240.6718.38pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 311.550.5819.87pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 311.380.4923.04pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 38.325.271.579pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 318.086.332.857pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 38.835.121.724pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 318.082.28.22pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 313.910.4928.33pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 314.671.847.973pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 312.770.7317.55pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosiduronic acid——0.06pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-glucopyranoside4.350.1529.80pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-mannopyranoside2.6524.20.109pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)Sulfolobus solfataricus4-nitrophenyl beta-D-xylopyranoside3.955.10.774pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-methylumbelliferyl beta-D-fucopyranoside180.3453pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-methylumbelliferyl beta-D-galactopyranoside5.40.4711pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-methylumbelliferyl beta-D-glucopyranoside5.10.3415pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-methylumbelliferyl beta-D-mannopyranoside2.80.93.2pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-methylumbelliferyl beta-D-xylopyranoside2.81.262.2pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-fucopyranoside——0.068pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosidebuffer; Table 20.0470.810.058pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 30.0561.30.043pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 30.0671.650.04pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosiduronic acid0.0043.460.001pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-glucopyranoside0.0180.510.036pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-mannopyranoside——0.001pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant E432CSulfolobus solfataricus4-nitrophenyl beta-D-xylopyranoside0.02813.70.002pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-methylumbelliferyl beta-D-fucopyranoside310.4176pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-methylumbelliferyl beta-D-galactopyranoside14.62.26.3pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-methylumbelliferyl beta-D-glucopyranoside331.6120pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-methylumbelliferyl beta-D-mannopyranoside0.920.185.1pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-methylumbelliferyl beta-D-xylopyranoside1.50.592.5pH 6.5, 80 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-fucopyranoside9.4318.60.506pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosidebuffer; Table 20.085.280.015pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 30.0818.30.004pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosideTable 30.1912.50.015pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-galactopyranosiduronic acid0.0042.720.00129pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-glucopyranoside2.7222.10.123pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-mannopyranoside——0.005pH 6.5, 45 °CPMID 15846595Open
Sulfolobus solfataricus beta-glycosidase (SsβG)variant W433CSulfolobus solfataricus4-nitrophenyl beta-D-xylopyranoside——0.003pH 6.5, 45 °CPMID 15846595Open
β-D-galactosidaseArthrobacter sp.lactose1.7677.540.023pH 6.5, 10 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.lactose2.3667.820.035pH 6.5, 20 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.lactose4.8152.670.091pH 6.5, 30 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.lactose5.7344.310.129pH 6.5, 40 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.lactose6.9839.730.176pH 6.5, 50 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.o-nitrophenyl-β-D-galactopyranoside52.45.759.12pH 6.5, 10 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.o-nitrophenyl-β-D-galactopyranoside814.8616.7pH 6.5, 20 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.o-nitrophenyl-β-D-galactopyranoside123.93.4635.81pH 6.5, 30 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.o-nitrophenyl-β-D-galactopyranoside169.93.1553.92pH 6.5, 40 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.o-nitrophenyl-β-D-galactopyranoside212.42.6281.07pH 6.5, 50 °CPMID 19631003Open
β-D-galactosidaseArthrobacter sp.o-nitrophenyl-β-D-galactopyranoside71.25.1113.93pH 6.5, 55 °CPMID 19631003Open
β-galactosidaseEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; continuous assay at 420 nm6200.125.2 × 10³pH 7, 25 °CPMID 8662937Open
β-galactosidaseEscherichia coli2-nitrophenyl beta-D-galactopyranosideno Mg2+ (Mg2+ omitted, 10 mM EDTA)390.5768 calc.pH 7, 25 °CPMID 8662937Open
β-galactosidaseEscherichia coli4-nitrophenyl alpha-L-arabinopyranoside21111.618.2pH 7, 25 °CPMID 8662937Open
β-galactosidaseEscherichia coli4-nitrophenyl beta-D-fucopyranoside123.416.97.3pH 7, 25 °CPMID 8662937Open
β-galactosidaseEscherichia coli4-nitrophenyl beta-D-galactopyranoside900.0412.2 × 10³pH 7, 25 °CPMID 8662937Open
β-galactosidaseEscherichia colio-nitrophenyl-ß-D-galactopyranoside6200.125.2 × 10³ calc.pH 7, 25 °CPMID 19472413Open
β-galactosidaseEscherichia colip-nitrophenyl-ß-D-galactopyranoside900.042.2 × 10³ calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418EEscherichia colio-nitrophenyl-ß-D-galactopyranoside150 mM Na+5.41040.052 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418EEscherichia colio-nitrophenyl-ß-D-galactopyranoside2 M Na+24.60.22110 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418EEscherichia colip-nitrophenyl-ß-D-galactopyranoside150 mM Na+5.413.20.41 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418EEscherichia colip-nitrophenyl-ß-D-galactopyranoside2 M Na+220.21100 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418FEscherichia colio-nitrophenyl-ß-D-galactopyranoside10.430.60.340 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418FEscherichia colip-nitrophenyl-ß-D-galactopyranoside10.320.80.495 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418NEscherichia colio-nitrophenyl-ß-D-galactopyranoside4.90.0682 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H418NEscherichia colip-nitrophenyl-ß-D-galactopyranoside5.50.05110 calc.pH 7, 25 °CPMID 19472413Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; continuous assay at 420 nm0.660.1414.7pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranosideno Mg2+ (Mg2+ omitted, 10 mM EDTA)0.040.670.060 calc.pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: Methanol (1 M)3.4——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: Glycerol (125 mM)3.1——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: L-Arabinose (250 mM)1.1——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Galactose (250 mM)2.2——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Fucose (250 mM)1.4——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Talose (100 mM)0.7——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: 2-Deoxy-D-Galactose (150 mM)1.4——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Glucose (300 mM)6——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli4-nitrophenyl alpha-L-arabinopyranoside8.261.3pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli4-nitrophenyl beta-D-fucopyranoside14.113.41pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540EEscherichia coli4-nitrophenyl beta-D-galactopyranoside1.20.52.4pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; continuous assay at 420 nm0.10.110.91pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranosideno Mg2+ (Mg2+ omitted, 10 mM EDTA)0.021.910.010 calc.pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: Methanol (1 M)0.2——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: Glycerol (125 mM)0.21——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: L-Arabinose (250 mM)0.09——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Galactose (250 mM)0.16——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Fucose (250 mM)0.12——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Talose (100 mM)0.04——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: 2-Deoxy-D-Galactose (150 mM)0.16——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Glucose (300 mM)0.44——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli4-nitrophenyl alpha-L-arabinopyranoside11.90.5720.9pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli4-nitrophenyl beta-D-fucopyranoside8.52.92.9pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540FEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.10.450.22pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; continuous assay at 420 nm0.70.0888pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranosideno Mg2+ (Mg2+ omitted, 10 mM EDTA)0.030.60.050 calc.pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: Methanol (1 M)1.4——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: Glycerol (125 mM)1.89——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: L-Arabinose (250 mM)0.7——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Galactose (250 mM)0.75——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Fucose (250 mM)0.58——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Talose (100 mM)0.56——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: 2-Deoxy-D-Galactose (150 mM)0.56——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli2-nitrophenyl beta-D-galactopyranoside1 mM Mg2+; acceptor present: D-Glucose (300 mM)3.71——pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli4-nitrophenyl alpha-L-arabinopyranoside20.732.7pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli4-nitrophenyl beta-D-fucopyranoside4.21.23.5pH 7, 25 °CPMID 8662937Open
β-galactosidasevariant H540NEscherichia coli4-nitrophenyl beta-D-galactopyranoside0.30.0694.4pH 7, 25 °CPMID 8662937Open
β-galactosidase 1 (OsBGal1)Oryza sativa4-nitrophenyl beta-D-fucopyranoside107728.537pH 5, 30 °CPMID 41978877Open
β-galactosidase 1 (OsBGal1)Oryza sativa4-nitrophenyl beta-D-galactopyranoside1220.2596pH 5, 30 °CPMID 41978877Open
β-galactosidase 1 (OsBGal1)variant ΔCter (OsBGal1ΔCter)Oryza sativa4-nitrophenyl beta-D-fucopyranoside7735.78133pH 5, 30 °CPMID 41978877Open
β-galactosidase 1 (OsBGal1)variant ΔCter (OsBGal1ΔCter)Oryza sativa4-nitrophenyl beta-D-galactopyranoside1063.9527pH 5, 30 °CPMID 41978877Open
β-galactosidase 1 (OsBGal1)variant ΔCter (OsBGal1ΔCter)Oryza sativa4-nitrophenyl beta-D-glucopyranoside2868.5633pH 5, 30 °CPMID 41978877Open
β-glycosidase (Ssβ-gly)variant H150ASulfolobus solfataricus2Np-Gal13524.75.5pH 6.5, 65 °CPMID 31072150Open
β-glycosidase (Ssβ-gly)variant H150ASulfolobus solfataricus2Np-Glc128513.595.2pH 6.5, 65 °CPMID 31072150Open
β-glycosidase (Ssβ-gly)variant H150ASulfolobus solfataricus4Np-2d-Glc44.24.3810.1pH 6.5, 65 °CPMID 31072150Open
β-glycosidase (Ssβ-gly)variant H150ASulfolobus solfataricus4Np-Glc805.923.9433.7pH 6.5, 65 °CPMID 31072150Open
β-glycosidase (Ssβ-gly)variant H150ASulfolobus solfataricusCellobiose30.4549.90.06pH 6.5, 65 °CPMID 31072150Open
β-glycosidase (Ssβ-gly)variant H150ASulfolobus solfataricusLactose35.8442.30.08pH 6.5, 65 °CPMID 31072150Open