Values CatLog read from this paper
Direct and indirect roles of His-418 in metal binding and in the activity of beta-galactosidase (E. coli)
PMID 19472413doi:10.1002/pro.140Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: β-galactosidase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| β-galactosidase | Escherichia coli | o-nitrophenyl-ß-D-galactopyranoside | 620 | 0.12 | 5.2 × 10³ calc. | pH 7, 25 °C | Open record: β-galactosidase, o-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidase | Escherichia coli | p-nitrophenyl-ß-D-galactopyranoside | 90 | 0.04 | 2.2 × 10³ calc. | pH 7, 25 °C | Open record: β-galactosidase, p-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418E | Escherichia coli | o-nitrophenyl-ß-D-galactopyranoside150 mM Na+ | 5.4 | 104 | 0.052 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418E, o-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418E | Escherichia coli | o-nitrophenyl-ß-D-galactopyranoside2 M Na+ | 24.6 | 0.22 | 110 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418E, o-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418E | Escherichia coli | p-nitrophenyl-ß-D-galactopyranoside150 mM Na+ | 5.4 | 13.2 | 0.41 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418E, p-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418E | Escherichia coli | p-nitrophenyl-ß-D-galactopyranoside2 M Na+ | 22 | 0.21 | 100 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418E, p-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418F | Escherichia coli | o-nitrophenyl-ß-D-galactopyranoside | 10.4 | 30.6 | 0.340 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418F, o-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418F | Escherichia coli | p-nitrophenyl-ß-D-galactopyranoside | 10.3 | 20.8 | 0.495 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418F, p-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418N | Escherichia coli | o-nitrophenyl-ß-D-galactopyranoside | 4.9 | 0.06 | 82 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418N, o-nitrophenyl-ß-D-galactopyranoside |
| β-galactosidasevariant H418N | Escherichia coli | p-nitrophenyl-ß-D-galactopyranoside | 5.5 | 0.05 | 110 calc. | pH 7, 25 °C | Open record: β-galactosidase variant H418N, p-nitrophenyl-ß-D-galactopyranoside |